LOCUS       sequence-221008-        4445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: LOC110678930 NPY receptor
FEATURES             Location/Qualifiers
     source          1..4445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2333..2467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2370..2389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2424..2443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2506..2523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2677..3265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2757..2776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3436..4227,4228..4296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4059..4078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4297..4401)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4441..4445,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tggcattcca aattagtgaa tcggcgttcc agtatttgtc
      841 atcgaacaac atcactgcag caaactttca ggatttggat gccagtccga gggaccagtt
      901 gattggatat tgggaaaatt cgcttaagaa tcttagcacg gaacagagga cggtttttac
      961 ggtgctggtt attctggtga tggtactggc catattcggc aatgtggtga cgtttattac
     1021 caatattcgg agggaacaac ggcatttgtt tcgcgtgtgt ctactctccc tggcgttgag
     1081 cgatatactt ttcgtaacaa ttacgtcaat agtgtatttg tcgcagttta atactgaata
     1141 caattcattg tggaccctcg gagagctaat gtgcacgttt gcccccttct tccaaacgat
     1201 ggccgtgctg gtggatagta tcactttggt ggcgatcgct ctggaccggt acatggctgt
     1261 ggtgcgactg accaagggag cttgggaacc cagcggcgta ttgtgcgtgg cctgtgccgt
     1321 tctgatatgg ggccttgcag ccggagtatc cagcccaatg ctaaccttgt accagatcta
     1381 ccacgttatc gtactcatag ccgatagaac ccattcagac gtgattacgg gttacttttt
     1441 agcagaaata tgcgccaccg ataagagcaa aaatggatac tactttggaa ttgtgttcac
     1501 tgtgatcttt ctaccgctgt tattatcatt cctttggctc aactctatca tcgctaaaga
     1561 aatctggatt cgaaggaacc cacttgacaa tcgcagcaaa gtgcataaga aatccaaaac
     1621 tggcatgttc accgacagca caggggaacg caaaaccacc tcaaccaaca tcagctcgag
     1681 caatgcttcg gatcgtcgtc ctccttcatt tccggtacgt cacaccgctt actccgtctc
     1741 taagtgtgtg tgctccaatt gcgtagatca acactcgccc ccacaggtgc ctcccccacc
     1801 acctcccaca gctcccagca ccagcccggc gtccaacaat cggaaaaagc gccagctgcg
     1861 catgttcaaa gccatcgtgt ttatcatgtc ggtgtttttc ctgtgtcgat tgccgacctg
     1921 gatattcctg ctgataaagc tgcacggcac tgccaacaca aacaccttct ggatgctgca
     1981 ctacattttt gggataatgg ccatgctgaa ctgcgtgctg aatccgctga tctacacctt
     2041 cctgggggag acgatcaagg tgacggtgtt tctcaaaacg atgtgcgaac gcttttgggt
     2101 ggatccttgc cgccgaggag ctcaacgggt gaggaccaag ttctctgagc atgtacacag
     2161 tggcaaaggg gaagttacac tgcgggtggg gaagaagcga ccgcatcaac agcaaaagca
     2221 acgtgacgga atgtttagtg gagattgagc ggccgctaga ctagtctaga gaaaaaacct
     2281 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2341 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2401 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2461 ctggatcccc gggtaccgag ctcgaattaa ttcctcttcc gcttcctcgc tcactgactc
     2521 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2581 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2641 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2701 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2761 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2821 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2881 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2941 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3001 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3061 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3121 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3181 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3241 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3301 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3361 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3421 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3481 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3541 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3601 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3661 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3721 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3781 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3841 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3901 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3961 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4021 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4081 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4141 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4201 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4261 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4321 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4381 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcaa gcttggctgt
     4441 ggaat
//
