LOCUS       sequence-221017-        5042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL017414 TK R
FEATURES             Location/Qualifiers
     source          1..5042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2930..3064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2967..2986)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3021..3040
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3103..3120)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3274..3862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3354..3373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4033..4824,4825..4893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4656..4675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4894..4998)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(5038..5042,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgaactacaa gaagaacctg ctgctcctgt acgaccgccc
      841 ccgggaaccc atcttcatgg gcaagggcaa gtcggtgttc gacgtgccgg ataactatct
      901 cacggatcgt taccgcccga ttggaccgga gattcagaac cgcttcggtg aactggcgga
      961 ggaacgcatc ccggtgagga gcattgcctt gccggatctg cgcattccga tgtctttggg
     1021 gcggcaggag cagttttcgc tgttcattcc gcggcaccgg aagattgcgg cacggctgat
     1081 cgacatcttt atgggaatgc gcaacatcga ggaactccaa agctgtgcag tgttcgctcg
     1141 ggatcgcatc aacccgtacc tgttcaacta cgccctctcg gtggcactgc tgcaccgtag
     1201 ggacaccaaa aacctcgacc ttccgtctgt agtcgaggtc ttcccagaca agtatgtcga
     1261 ctcgcgagtt ttcgaacaga tccgcgaaga agccacggtg gtccccgagg gcatgcgtat
     1321 gcccatcgtc atccccaagg actttaccgc ttcggatctg gacgaggagc accgcctgtg
     1381 gtacttccgc gaggacatcg gagtcaacct gcaccactgg cactggcatc tggtgtatcc
     1441 gtttgaggct tcgaatcgtg cgatcgtcga taaggaccgc cgcggtgaac tgttctacta
     1501 catgcattcc cagttgatcg cgcggtacaa cttcgagcga ttctgcaatc gcttacaacg
     1561 cgtcaagcgg ttgaacaacc ttcgcgagcc aatcgctgag ggatatttcc ccaaattgga
     1621 ctctctggtg gcgtcccgta cctggcccgg tcgggtggac aacgccgtaa tcaaggatct
     1681 gaaccgcgag ctggaccaga tcaagcagga cgtgtcggat ctggaacgct ggatcgaccg
     1741 tatctacgaa gccgtccacc agggctacgt ggtggacgaa tccggcaatc gcatctttct
     1801 ggacgaagag aagggaatcg acatcttggg taacatcatc gagtcgtcga ttttgtcgcc
     1861 caatcggcag ttgtacggag acatgcacaa cgtgggtcac gtgttcctgt cgtacactca
     1921 cgatccggat catcgacatc tggaatcttt tggagtgatg ggtgacgtag ctacggctat
     1981 gcgtgatcca gtgttctacc gttggcactc gttcatcgac gacatcttcc aggagcacaa
     2041 gattaaactg ccggcgtata ccaagagtca gctcacctac gaaggtatat ccgtaacggg
     2101 tatcatcgtc caatcggaag gagccccggt caatactctg cacacatact ggcagcagtc
     2161 cgatgtggat ttgtcgcgtg gaatggattt tgtcccacgg ggtaacgtgt ttgcaagatt
     2221 cacccatctg cagcacgcac ccttccagta cgtcatccag atcgacaaca cttccgatgc
     2281 acaacgtatg ggcttcgtgc gaatcttcat ggccccgaag aacgatgaac gtggccaacc
     2341 gatgctgttc cgagaccagc gtctattcat ggtcgagatg gataagttcc tggtggcact
     2401 tcgtcccggt gccaatcgca ttcgacgccg atcgaacgag tccaccgtaa ccattccgtt
     2461 cgagcggacc ttccgtaacc tggacgataa gcgaccggaa acgggcactc ccgaggagga
     2521 agcctttaac ttctgtggtt gcggatggcc cgcccacatg ttggtgccca agggtctacc
     2581 ggagggtttc ccggccgatc tgtttgtgat ggtttccaac tacgaggacg atcgggtggt
     2641 tcaggatctg gtcggaactt gcaacgacgc ggcttcgtac tgtggagtgc gcgataggct
     2701 ctatccggac cggaaggcca tgggtttccc gttcgatcgg ctggcccgga ccggagtcga
     2761 taggctgtcc aattttgtga cgcccaacat ggctatccag tcggtgaacg tcatccatat
     2821 cgataagacc gtgcccagga cttaggcggc cgctagacta gtctagagaa aaaacctccc
     2881 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     2941 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3001 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3061 gatccccggg taccgagctc gaattaattc ctcttccgct tcctcgctca ctgactcgct
     3121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4021 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4081 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4141 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4201 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4261 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4321 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4381 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4441 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4501 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4561 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4621 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4681 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4741 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4801 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4861 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4981 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcaagct tggctgtgga
     5041 at
//
