LOCUS       sequence-221092-        5285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL004399 GPB5
FEATURES             Location/Qualifiers
     source          1..5285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    3173..3307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3210..3229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3264..3283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3346..3363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3517..4105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3597..3616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4276..5067,5068..5136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4899..4918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5137..5241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(5281..5285,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgctgcatat ctcgaaaaca aaattatata ataagccgca
      841 ccctaatatt acgcatccat taaaatgcaa tccgatccta ttgccggaga gcttttgtaa
      901 tcatcccatc gctcttgtct ttatttattt tctaccccgc tgtgctgccg cctgcccaaa
      961 cagagattta gacggtaatc agatccagga gatccacgag agagccattc aaatcaagac
     1021 tgatgaatta attttagata ataatgcact caccgacatc catggtgccg ctttcttcgg
     1081 ttcacaggta gctaaactca gtctgaagct caacttcaaa ctgaaacaca tccacccatc
     1141 cgccttcgtt ggaatgcgaa acatcagaga gttggaccta tccggaacgg cattggaagc
     1201 catgccgact gaaggcctca gcgagctgga ggttctgcgg attcagaaca cccactcgct
     1261 gaagaccatc ccgtcggtgt ataatttcaa gaacctgcaa acggcgtacc tgacgcattc
     1321 gttccactgt tgtgcgttcc aatacccggc ccgacatgac ccggacggcc acgaacaagc
     1381 cctgcgacga ttccatgaag tccagaaaaa gtgcatcgct aaaggttatt ccacaccgaa
     1441 cgtgaaagtg ggaagcttcg ccgaactggc aacagatggg accaaatcga tgagcgacca
     1501 aacagctgag ctggtttccg atggatggta ccgggaaaaa cgctacgccc atcccccgac
     1561 cccggttgac gaaggaaacg gccggttgcg tatcttttca agaaatagca tcaatttccg
     1621 accagatgag caacaagacg gtccagcagc ggccggttct ggagcagacc cggacgccag
     1681 tcttgcgaag agcgaactgg gcgaaagcta ctttcagccc atcaccggcc tgcccatggg
     1741 aagcatcctg caagaggacg tatccgatct gtacacggag gacagcctga tgggattttt
     1801 ccacgaaacg gaagtggaac cctcgtcgat ggaagcgatc tgcggaaacc ttacgcccac
     1861 aacatttgat gttaagtgct acccgatgcc ggacgcgctg aatccatgcg aggacgtaat
     1921 gggatcccac tggctgcgtg gttccgtctg ggttgtggtc ctgttggccg tgtttggaaa
     1981 tgttgcggtt ctggtggtat tgttctccaa caggaccgat ttaaccgttc cgaagtttct
     2041 tatttgccat ctggcctttg ccgatctgtg catggggctg tatctgctgc taattgcatc
     2101 gattgatgca cattccatgg gcgagtactt caactttgca ttcgactggc agtacggttt
     2161 cggttgcaag gtggctggct tcctaactgt ctttgcgagc catctatcga tcttcacgct
     2221 gaccatcgtc actctggaaa gatggttcgc catcacccac gccatctacc tgaacaaacg
     2281 catcaaactt tcggcggcca cctatatcat gattgccggt tggatctact ccgccgtaat
     2341 ggctgccttc ccgctgtttg gcattagcaa ctattcatca acaagcatat gtttgcccat
     2401 ggaagcccgg gacacctggg atattgccta ccttctgacg gtgctgggaa tcaacggatt
     2461 ggccttcatc atcatcgtca tctgctacgc tcagatctat ttttcgctcg gcaaggaaac
     2521 tcgccaggct gcccgatcaa ctcacagcgg agaaatgact gtggcgaaaa agatggcact
     2581 tctggttttc acaaactttg cctgctgggc accgatagct ttcttcggac tgacagccat
     2641 tgccggatat ccgctgattg atgtgaccaa gtctaaaata ttgctggtgt ttttctatcc
     2701 gctgaactcg tgtgcaaatc catacttgta cgctattctg acgatgcagt accggaagga
     2761 cttcttcacg ctgctatcca attacggttt gtgtacccaa cgtgctcaac aatacaaaat
     2821 gacctactca ttaccgacaa atacgatcca aattgtgccg gcaaggggct ccataaccac
     2881 caccctcaat accacatcga atacgaatgg aacaactttc agtcaggtgg ctagcagttg
     2941 tactaatatg gaccaatccc gtgccaatag tttgaatggc cacgtcacaa acgacacagt
     3001 ggtggaagag gtgaatgtga aaccggatag aatcaacagt ctggccagca gtgtcgtcga
     3061 ggagcaggaa agtgaaactt ttctgtaagc ggccgctaga ctagtctaga gaaaaaacct
     3121 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3181 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3241 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3301 ctggatcccc gggtaccgag ctcgaattaa ttcctcttcc gcttcctcgc tcactgactc
     3361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcaa gcttggctgt
     5281 ggaat
//
