LOCUS       sequence-221098-        4844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL003747 PK R
FEATURES             Location/Qualifiers
     source          1..4844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2732..2866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2769..2788)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2823..2842
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2905..2922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3076..3664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3156..3175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3835..4626,4627..4695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4458..4477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4696..4800)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4840..4844,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tggtaacggc cgccacgagc tcgagtttcg tcacggcaac
      841 cgatggagac gcgcccggag tggatggctt cacgacgaga aacggaaccg gcgtggatgt
      901 tctgctccag ttgccggccg tcgtttcgat caacggttcc actggaaacg gcgaagtgat
      961 gaatgaaaac tttggcacca aagccgattc catctgcatc atactgccca ttaccatctt
     1021 ctattgcttc atcttcgtcg tcggcattgt gggaaacctg gccatctgta ttgtgattgc
     1081 caagaatcgg tcgatgcaca cggccaccaa ttactatctg ttcaatttgg ccgtttcgga
     1141 cttcctgctg ctgctgttcg gcatgcccct ggaggtctac ggaacgtggt atccttatgc
     1201 atatccattt aaccaagtgg cttgcatcat caccgggctg ctctcggaga cggccaccaa
     1261 cgccaccgtg ctgacaataa cttccttcac cgtagaacgc tacatcgcca tctgccatcc
     1321 gtttcgatca catactatgt ccaagctttc gcgggcaatc aagttcgtca tcgcaatctg
     1381 gctggtggcg ttcggattgg ccaccccaca ggcgctgcag ttcggcgtgg tggaaagcgc
     1441 tcacacgagg ctctgtacga tcaaaaaccg tcacttcgag cacgccttcg aggtgtccag
     1501 tttcctgttc ttcgtcggtc cgatgacggt catcgccgtg ctgtacgtgc taattgggat
     1561 caaactccgc aagagcaagc tgctgcaggg cgtcaaacgg cagggggcca gcatcggaag
     1621 cggcgcaaac ggcatccatc atcacggcgg tggaatgacc acttccggcg gtggcgctgg
     1681 ctttcggtcc gtcagcggac agacacgtgt gatacgaatg ctcgtggccg tggtggcgac
     1741 gttctttttc tgctgggcac cgttccacgc acaacgactg atggccgtgt acggagccgt
     1801 taccaacacg gataatgagt ttttcttcca ggtgtacacc tatctaacat acatatccgg
     1861 catattgtac tttctgtcga cgtgcatcaa cccgctgctg tatcatatca tgagccacaa
     1921 atttcgggac gcttccaggc gcaccctcaa actgggctgc tgtggcatcg attcgaagcg
     1981 gtccgacgcc cagaaccaca cctacagtgc cctctcgcgg tacggaataa ccggcaacgg
     2041 aggctccttc aaaacgggcg caaatgctca atacggtact ccggtagccc aaaatcgccc
     2101 gagcacggac atgcagttct gccagcaaga gtcggaaatg tccctactac ggagcgaacc
     2161 cggtcgttgt ttgaagtccg aggcgcactg cgtctccatc agcagtcaat caacgatcat
     2221 aaccaccctg agccacagtg gtactacgcg atcgtcctcg atcgagcgga aacaacccaa
     2281 cggacgtccc gtctaccgca cttccggaag tttcggatcg cgagggagca ttcgcaccgg
     2341 agtaccacta gagaacggag acccgggcgg tcgactgtcc accattgcgg agaagctacg
     2401 ccgaggcacc aagaaagtac tccagttcag caaatctccg tcaacctcgc cgacgaagac
     2461 ttcaaccgga agcgtatccg cgcccgatcg ggacgccaaa cgacgctggt tacgcaagaa
     2521 ggaaagcgtc gactcggtcg acaccaacac gataagcaat tccagtctga aggagtacga
     2581 cgaagaggag ttttccagtg cggaactagc taaatttatg gccgaaatca acaatgagat
     2641 tcgctgagcg gccgctagac tagtctagag aaaaaacctc ccacacctcc ccctgaacct
     2701 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     2761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2821 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccccg ggtaccgagc
     2881 tcgaattaat tcctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4741 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4801 cacatttccc cgaaaagtgc cacctgcaag cttggctgtg gaat
//
