LOCUS       sequence-221126-        5860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL008296 CCAP R
FEATURES             Location/Qualifiers
     source          1..5860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    3748..3882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3785..3804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3839..3858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3921..3938)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4092..4680)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4172..4191)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4851..5642,5643..5711))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5474..5493
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5712..5816)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(5856..5860,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgccgaagat cagcttgatt aagcaccgga tccaccagga
      841 gcagttgcgg ctgctcgagt cccagaagaa tgccaacttt ggcgaggggg gcttcgggag
      901 cgaaccgctg tcgctggtat cgcgcaagaa ggaccacaaa gagggcgcag atcgcgacat
      961 cttcagcgag gagaaccgcc tgagtccgcc atgccgcgag acgtcctcct cgccctcatc
     1021 gtccatcttc agctcggtcg tccaagcggc ccagaacctg tcagtcaacc agagaagcag
     1081 tcccggctca ccgccggcgg tgcccctgtt tctccagcgc agcttcgcca aagaaccttc
     1141 ggaacccgca cccatcccgg ccccgacgcc ggggcgtcgc ttcttctcgt ccatcaccgg
     1201 cggcgacatc ccctacggcg gaagccgccc aggacacgtg ctgacacagg ccgagcgcaa
     1261 agtctacccc ccaagccggc ccctcactcc tcagcagcaa caacccccgg tggagaagaa
     1321 ggatccccac agcaatcagt cacagccggc ggcccgcgag gaacgcaatg tcatcacgga
     1381 ggaacgtccg gtggtctcgg tggaaccacg atcaccaccc ccaaaagacc gcgtcccgac
     1441 gccaccccca gcgccaggac ccgtcgtgcg ctgctccgtc atccagcgag ccccacaagc
     1501 ccctctgtcc ccacggagac ccacagtgtc cagcccggac agcagcccca aggcagtgga
     1561 tctgaaggtg cgccccacag agctggaacc cgagcaggaa cagcccatcg attaccacat
     1621 accaaaacgc tgcgaggacg gcaaggagaa caagaaggag gacgaagagc gggagaagaa
     1681 gatgcgcgag gcccgacggg aggccatcct gaggagacat ctcctctcaa tgcgctaccg
     1741 atacggtaag ctgaacggga tcatgcaggc cgcggccgga cacggtcgct cctcaagcgg
     1801 cggaggacaa gccggaggcg gtcacagcgg acacagcggt ggcggaagta tcaacttctc
     1861 ttccggatcc ggaagccttg gaggagctag cggaggcagt ggcggtgcaa tcggtggagg
     1921 tggaggagcc ggaggtggca tgggaggagg acgcgacgga cggtccaact atggcccgaa
     1981 cagccctcca accggatctc tgccaccctt ctacgaaagc ctcaaaggcg gcaacgctgg
     2041 gctcaacggc tacaacgcca acggaggctt cctcaacacc aactggaaca acatggactg
     2101 cgacaccact caggatctaa ccaacatagg agcctacacg gatgctaacg ggaacagcag
     2161 tacgaacccg tccaaacaat actccatgct tcagaacgcc tacggcatag cgctcaagga
     2221 cgaagcagac ctcgactacg actccaagat ggactcgttc tccaacctct acggcaacta
     2281 cgacgtcaac gattccatga tggccgaaat gaacggcgtc ggtgtcgacc cgctgcacct
     2341 caccgcgaca ctcaccttct cctcgccggg agacagcgcc ctgctggaca gtttcaccga
     2401 cgccgtcgac ctgagccact tcctgaatcg tctcccctcg gacgagcagt ccagtagctg
     2461 caacgatctg gaactaacct ccaccccatc gctcaccccc gattccgtgt ccacctccct
     2521 ccaacaggtc gaccaccacg acggcctcga ccaatacccg gaacacctgc tcctctcccg
     2581 aagctacaac gccaccttca acaacacccc caaatatatg caaccaccgc acccacacca
     2641 agagaatccc cccagctacc aatcctccca ccagttccac tccctcagca acttcgacct
     2701 cgactcccac agtaacctgt cccttccctc ccccaacagc cactccctcc aatccccctc
     2761 ctcatcctcc tctccaccat cttcctcctc atcatctgcc accgcagcag cagcagccgc
     2821 cgccgccgcc gcatccctag ccgccgccgg cctcccgggc catggccccc acgtagtggc
     2881 tcttggtgcc ccatcaccgg tcaccgcacc gcccccgggc gtcgtcgcct caaccatcca
     2941 aaacctcggc ctcccggcgg aagtccagct ggagttcgtg aacggtggcc acggcatcaa
     3001 gaaccccctc gcggtcgaaa acatccccgg ccggctccgg gacgaggaca aacaccactc
     3061 gaagctgctc cagcaaacgg aagtcctgaa agtctccatc gatgaaaccg gcgccgacca
     3121 gcagagcaag ttcgtctgcc ggatatgcca gaagtcgttc acgttgcagc ggttgctcaa
     3181 ccggcacatg aagtgccact cggacgtcaa gcggtacctg tgcacgttct gcggcaaggg
     3241 gttcaatgat acgttcgacc tgaagcggca cacccggacg cacaccggcg tccggcccta
     3301 caagtgcaat ctgtgcgaga agtcgttcac gcagcggtgc tcgctggagt cgcactgttt
     3361 gaaggtgcac ggcgtgcagc accagtacgc gtacaaggag cggcgcacga agatgtacgt
     3421 ctgcgaggag tgcggccaca cgaccaacga accggaggtc cactacctgc acctgaagga
     3481 gaagcacccc tactcgccgg ccctgctcaa gttctacgac aagcgccact tcaagttcac
     3541 caactcacag ttcgccaaca atctcctggg ctcgttcccg atgcctgttc agaactgaat
     3601 ttgatcgttt ttttgtattt gtgcagtgga acgcgcacac ctttatacgc gcacaaccag
     3661 taggcggccg ctagactagt ctagagaaaa aacctcccac acctccccct gaacctgaaa
     3721 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     3781 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3841 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccccgggta ccgagctcga
     3901 attaattcct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4861 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4921 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4981 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5041 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5101 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5161 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5221 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5281 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5341 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5401 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5461 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5521 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5581 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5641 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5701 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5761 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5821 tttccccgaa aagtgccacc tgcaagcttg gctgtggaat
//
