LOCUS       sequence-221134-        4187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL010043 DH31R
FEATURES             Location/Qualifiers
     source          1..4187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2075..2209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2112..2131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2166..2185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2248..2265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2419..3007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2499..2518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3178..3969,3970..4038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3801..3820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4039..4143)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4183..4187,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attcctcgag tggatgacat cctcaacaac gacggacgca accgattcgg agttcgacca
      841 gaggaagctt caatgcttgg aaatgctcaa cgcaaccacc gaattcacca caacccggtc
      901 tggacctttc tgcaggggaa cctgggatgg ttggctctgt tggccggata ctgcggctgg
      961 cgaatctgca ctgctaccct gcccagattt catggatgga ttcgatccaa caagattcgc
     1021 acacaaagac tgcgatgagg acggagaatg gtttaggcat ccactgacca acagaacttg
     1081 gtccaattac acaacctgcg ttaatttgga taagctcgag tggatggaac aagtgagaac
     1141 gatatacgaa acgggatact cgatttcgct gatagccctc atcttatcgc ttggtatttt
     1201 aagttacttt aggtcactga aatgcgctcg cattacgcta catatgaacc tgtttgcgtc
     1261 gtttgcctca aacaacacac tctggctgct gtggtaccgg atggtgctgg cagatccgga
     1321 agttctcaag cacaatgggg cgtcctgcat cacactgcac ctggtgctgc actacttcct
     1381 catcaccaac tacgcgtgga tgctctgcga aggattctat ctgcacacgg tcctggtgtc
     1441 ggcattcgtt tcggagaaaa aactcgtcaa ctggcttgtg gtcctcggat ggaccacacc
     1501 ggggatcgtg atcatggcgt atggatttct gcgaggatac gccggtaccc cggaggatac
     1561 gatagagtgc tggatgaacg aaagcgtgta tgataacgtt ttcaaggcac cggtgtgcat
     1621 ctcgatgctg ctcaatttgc tattcctgtg caatatcata cgagtagttc tgctgaagtt
     1681 gaaagctcct gcgggaccgc aaggtaccgg gccatcgaga accatactgc aagcgttcag
     1741 agccactctg ctgctagttc cgcttcttgg cctacagtac atattgactc cgttccgacc
     1801 cgatcctgga cattcttatg agcgaacgta cgaaattata tcggcgttca cggcatcatt
     1861 ccaaggtcta ttcgtagccg tcctgttctg tttcttcaac ggagaggtta tcgcacaagt
     1921 gaaacggaaa tggcgcaccg tgttcttgcg gactcgaacc aattcctaca ccgcaacgca
     1981 agtgtcctaa gcggccgcta gactagtcta gagaaaaaac ctcccacacc tccccctgaa
     2041 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2101 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2161 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ccgggtaccg
     2221 agctcgaatt aattcctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2281 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2341 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2401 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2461 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2521 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2581 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2641 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2701 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2761 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2821 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2881 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2941 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3001 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3061 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3121 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3181 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3241 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3301 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3361 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3421 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3481 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3541 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3601 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3661 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3721 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3781 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3841 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3901 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3961 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4021 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4081 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4141 gcgcacattt ccccgaaaag tgccacctgc aagcttggct gtggaat
//
