LOCUS       sequence-221139-        4352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: LOC5578735 SIFa receptor
FEATURES             Location/Qualifiers
     source          1..4352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2240..2374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2277..2296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2331..2350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2413..2430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2584..3172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2664..2683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3343..4134,4135..4203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3966..3985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4204..4308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4348..4352,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tggaaaccaa tttaattaac tgttctacca gcatcggatc
      841 gcaccagaac aattggacct gcttcgtcgt gcactatgag ccggaatcga tggcatctcc
      901 atcgctgtct acgacgacca cgttggccaa cctcagccag tcacagtttg acgaactgga
      961 agcggaagtg gaagtggact acgacgaccc gctggtaatc gaccctgcct acgaactacg
     1021 atacgggctg gttgggacgg ttttgctaag cgtggcctat gctctgatat tcatcgtcgg
     1081 actgctgggc aacatctcgg tggtgattgt cgtgaagaaa agcaccatga tgcgatctcc
     1141 gaccaatcgc ttcatcgtca atctggcgta cgctgattta ttggtcaatt tcctctgtct
     1201 gccgttcacg ctcattggca acatctatcc ggcttggatc cttggggtgt tcttctgcaa
     1261 gaccgtatcc tatcttcagg gcgtttcggt gtcggcatcg gtcaacacgc tgatggccat
     1321 ctcgattgag cgctgcgtgg ccatttcgtt ccctatctcc ggtaccatca ccaccagaca
     1381 gtaccgggtg attgttaccg tcatctggat gatagcgctg ggtatcaatc ttccgtggtt
     1441 gttcgttttt acgttgaaac cgataggcat tccgggcagt ttggcgcaga tttgcaccga
     1501 actatggcct tccccggcat cggagagctg gtactttctg tttgccaatc ttttcctgtg
     1561 ctacctggga cccctcattg tgatttcgat ttgctatgtc atcatctgga aaaatgtcgc
     1621 ctatcggaag ctcccacgtg acctgattat cagccggaaa aacgaagtct acaatcgaag
     1681 caaggtgaag gtcatcaaaa tggtattcgt tgtaatcatc actttcgcca tctcgtggct
     1741 tccgttgtac actattttct gcttcgtcaa gtttgctggc gatctgatat acgatgagtc
     1801 agattatctc tcgacagttc aagccatcat ctttggaatc ctgccagtag cgcaatggct
     1861 gggagcggcc aactcgtgca tcaatcccat cctgtatgca tttatgaata ggaaatttag
     1921 ggccggtttc cggcatttgt tccggtcctg ttgcaacaac gaagacgatg actgggacca
     1981 gccgacaacg gtgctacgaa gtgacgaaac acgtgatctg gtgctccggc ggatacgaac
     2041 cgagaggcat tttgtccgaa atggaaccag aaacggcctc gggccaccgc actgtaactc
     2101 tcttcggtac agtcgttcaa cgaaggtatc aatccagtcc catctagtcg attgagcggc
     2161 cgctagacta gtctagagaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2221 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2341 caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc gaattaattc
     2401 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2461 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2521 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2581 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2641 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2701 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2761 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2821 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2881 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2941 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3001 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3061 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3121 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3181 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3241 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3301 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3361 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3421 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3481 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3541 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3601 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3661 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3721 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3781 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3841 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3901 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3961 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4021 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4081 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4141 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4201 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4261 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4321 aaaagtgcca cctgcaagct tggctgtgga at
//
