LOCUS       sequence-221144-        8414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chemical induction of MAD1 localisation to a specific site in the 
            cell (by rapamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Kuijt TE, Omerzu M, Saurin AT, Kops GJ
  TITLE     Conditional targeting of MAD1 to kinetochores is sufficient to 
            reactivate the spindle assembly checkpoint in metaphase.
  JOURNAL   Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. 
            Epub 2014 Apr 4.
  PUBMED    24695965
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00412-014-0458-9"; journalName: "Chromosoma"; date: 
            "2014-10"; volume: "123"; issue: "5"; pages: "471-80"
COMMENT     Sequence Label: pcDNA5-eYFP-FKBP-MAD1-AA
FEATURES             Location/Qualifiers
     source          1..8414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    682..700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    703..721
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     731..755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      880..889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(886..888,889..891,892..1602)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(931..952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1192..1211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1539..1560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1648..1668
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1672..1992
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(4228..4245)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4234..4458
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4591..4610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4742..4789
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4797..5819
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4797..5819
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5949..6070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5986..6005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6040..6059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6119..6135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6119..6135)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6132..6154)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6143..6159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6167..6173,6174..6191,6192..6197))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6212..6233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6350..6367)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6521..7109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6601..6620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7280..8071,8072..8140))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7903..7922
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8141..8245)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8320..8339)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
       61 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
      721 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      781 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
      841 agcttggtac cgagctcgga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
      901 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      961 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1021 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     1081 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1141 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1201 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1261 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1321 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1381 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1441 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1501 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1561 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggagg ggatcggtgg
     1621 agctccaccg gtggcggccg ctctagagag aatctttatt ttcagggcgc cggagtgcag
     1681 gtggagacta tctccccagg agacgggcgc accttcccca agcgcggcca gacctgcgtg
     1741 gtgcactaca ccgggatgct tgaagatgga aagaaagttg attcctcccg ggacagaaac
     1801 aagcccttta agtttatgct aggcaagcag gaggtgatcc gaggctggga agaaggggtt
     1861 gcccagatga gtgtgggtca gagagccaaa ctgactatat ctccagatta tgcctatggt
     1921 gccactgggc acccaggcat catcccacca catgccactc tcgtcttcga tgtggagctt
     1981 ctaaaactgg aaagcttggg aggcggtagt atggaagacc tgggggaaaa caccatggtt
     2041 ttatccaccc tgagatcttt gaacaacttc atctctcagc gtgtggaggg aggctctgga
     2101 ctggatattt ctacctcggc cccaggttct ctgcagatgc agtaccagca gagcatgcag
     2161 ctggaggaaa gagcagagca gatccgttcg aagtcccacc tcatccaggt ggagcgggag
     2221 aaaatgcaga tggagctgag tcacaagagg gctcgagtgg agctggagag agcagccagc
     2281 accagtgcca ggaactacga gcgtgaggtc gaccgcaacc aggagctcct gacgcgcatc
     2341 cggcagcttc aggagcggga ggccggggcg gaggagaaga tgcaggagca gctggagcgc
     2401 aacaggcagt gtcagcagaa cttggatgct gccagcaaga ggctgcgtga gaaagaggac
     2461 agtctggccc aggctggcga gaccatcaac gcactgaagg ggaggatctc ggaactgcag
     2521 tggagcgtga tggaccagga gatgcgggtg aagcgcctgg agtcggagaa gcaggagctg
     2581 caggagcagc tggacctgca acacaaaaaa tgccaggaag ccaatcagaa aatccaggaa
     2641 ctccaggcca gccaagaagc aagagcagac cacgagcagc agattaagga tctggagcag
     2701 aagctgtccc tgcaagagca ggatgcagcg attgtgaaga acatgaagtc tgagctggta
     2761 cggctcccta ggctggaacg ggagctgaag cagctgcggg aggagagcgc gcacctgcgg
     2821 gagatgagag agaccaacgg gctgctccag gaagagctgg aagggctgca gaggaagctg
     2881 gggcgccagg agaagatgca ggagacgctg gttggcttgg agctggagaa cgagaggctg
     2941 ctggccaagc tgcaaagctg ggagagactg gaccagacca tgggcctgag catcaggact
     3001 ccagaagacc tttccagatt cgtggttgag ctgcagcaga gggagcttgc cttgaaggac
     3061 aagaacagcg ccgtcaccag cagcgcccgg gggctggaga aggccaggca gcagctgcag
     3121 gaggagctcc ggcaggtcag cggccagctg ttggaggaga ggaagaagcg cgagacccac
     3181 gaggcgctgg cccggaggct ccagaaacgg gtcctgctgc tcaccaagga gcgggacggt
     3241 atgcgggcca tcctggggtc ctacgacagc gagctgaccc cggccgagta ctcaccccag
     3301 ctgacgcggc gcatgcggga ggctgaggat atggtgcaga aggtgcacag ccacagcgcc
     3361 gagatggagg ctcagctgtc gcaggccctg gaggagctgg gaggccagaa acaaagagca
     3421 gacatgctgg agatggagct gaagatgctg aagtctcagt ccagctctgc cgaacagagc
     3481 ttcctgttct ccagggagga ggcggacacg ctcaggttga aggtcgagga gctggaaggc
     3541 gagcggagtc ggctggagga ggaaaagagg atgctggagg cacagctgga gcggcgagct
     3601 ctgcagggtg actatgacca gagcaggacc gcagtggcgc acatgagcct gaaccccacc
     3661 agtgtggcca ggcagcgcct gcgcgaggac cacagccagc tgcaggcgga gtgcgagcga
     3721 ctgcgcgggc tcctgcgcgc catggagaga ggaggcaccg tcccagccga ccttgaggct
     3781 gccgccgcga gtctgccatc gtccaaggag gtggcagagc tgaagaagca ggtggagagt
     3841 gccgagctga agaaccagcg gctcaaggag gttttccaga ccaagatcca ggagttccgc
     3901 aaggcctgct acacgctcac cggctaccag atcgacatca ccacggagaa ccagtaccgg
     3961 ctgacctcgc tgtacgccga gcacccaggc gactgcctca tcttcaaggc caccagcccc
     4021 tcgggttcca agatgcagct actggagaca gagttctcac acaccgtggg cgagctcatc
     4081 gaggtgcacc tgcggcgcca ggacagcatc cctgccttcc tcagctcgct caccctcgag
     4141 ctcttcagcc gccagaccgt ggcataagat atccagcaca gtggcggccg ctcgagtcta
     4201 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     4261 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     4321 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4381 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4441 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     4501 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4561 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4621 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4681 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct
     4741 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa
     4801 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     4861 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     4921 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     4981 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     5041 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     5101 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     5161 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     5221 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     5281 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     5341 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     5401 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     5461 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     5521 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     5581 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     5641 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     5701 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     5761 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     5821 acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5881 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5941 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6001 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6061 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6181 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6241 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6301 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7321 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7381 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7441 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7501 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7561 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7621 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7681 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7741 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7801 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7861 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7921 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7981 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8041 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8101 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8161 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8221 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8281 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     8341 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     8401 caaaatttaa gcta
//
