LOCUS       sequence-221151-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  TITLE     Johannes Schmid plasmids
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4xNFkB Luc
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(66..97)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    228..349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(239..258)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     293..312
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(480..502)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    588..728
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     643..662
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(743..760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggcgccggg cctttcttta tgtttttggc gtcttccatt ttaccaacag taccggaatg
       61 ccaagctgga attcgagctt ccattatata ccctctagag tctccgcgga gacagaggaa
      121 agtcccagca gaaagtttct cagcagagga aagtcccagg gcggagacag aggaaagtcc
      181 cagcagaaag tttctcagca gaggaaagtc ccagggccca gacatgataa gatacattga
      241 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      301 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaattg
      361 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      421 cctctacaaa tgtgatatgg ctgatcctgc ctcgcgcgtt tcggtgatga cggtgaaaac
      481 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
      541 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
      601 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
      661 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      721 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      901 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      961 caagtca
//
