LOCUS       sequence-221168-        7948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Jelluma N, Brenkman AB, McLeod I, Yates JR 3rd, Cleveland DW, Medema
            RH, Kops GJ
  TITLE     Chromosomal instability by inefficient Mps1 auto-activation due to a
            weakened mitotic checkpoint and lagging chromosomes.
  JOURNAL   PLoS One. 2008 Jun 11;3(6):e2415. doi: 10.1371/journal.pone.0002415.
  PUBMED    18545697
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2008-06-11"; volume: "3(6):e2415. doi"; pages: " 
            10.1371/journal.pone.0002415"
COMMENT     Sequence Label: pFastBac-GST-Mps1-WT
FEATURES             Location/Qualifiers
     source          1..7948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        197..288
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     213..230
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             331..984
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     942..964
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             991..1008
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     polyA_signal    3750..3884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3787..3806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3841..3860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3913..4078
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4262..4716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4349..4368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4558..4579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4742..4846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4847..4915,4916..5707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5065..5084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5878..6466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6367..6386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6620..6637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6771..6995
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7062..7595)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7765..7770,7771..7787,7788..7793))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc
       61 ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag
      121 gcgagcatcg tttgttcgcc caggactcta gctatagttc tagtggttgg ctacgtatac
      181 tccggaatat taatagatca tggagataat taaaatgata accatctcgc aaataaataa
      241 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat
      301 tcataccgtc ccaccatcgg gcgcggatcc atgtccccta tactaggtta ttggaaaatt
      361 aagggccttg tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag
      421 catttgtatg agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg
      481 gagtttccca atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc
      541 atcatacgtt atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca
      601 gagatttcaa tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca
      661 tatagtaaag actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg
      721 aaaatgttcg aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat
      781 cctgacttca tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg
      841 gatgcgttcc caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat
      901 aagtacttga aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt
      961 ggtggtggcg accatcctcc aaaatcggat ctggttccgc gtggatcgaa ttcaaaggcc
     1021 tacgtcgaga tggaatccga ggatttaagt ggcagagaat tgacaattga ttccataatg
     1081 aacaaagtga gagacattaa aaataagttt aaaaatgaag accttactga tgaactaagc
     1141 ttgaataaaa tttctgctga tactacagat aactcgggaa ctgttaacca aattatgatg
     1201 atggcaaaca acccagagga ctggttgagt ttgttgctca aactagagaa aaacagtgtt
     1261 ccgctaagtg atgctctttt aaataaattg attggtcgtt acagtcaagc aattgaagcg
     1321 cttcccccag ataaatatgg ccaaaatgag agttttgcta gaattcaagt gagatttgct
     1381 gaattaaaag ctattcaaga gccagatgat gcacgtgact actttcaaat ggccagagca
     1441 aactgcaaga aatttgcttt tgttcatata tcttttgcac aatttgaact gtcacaaggt
     1501 aatgtcaaaa aaagtaaaca acttcttcaa aaagctgtag aacgtggagc agtaccacta
     1561 gaaatgctgg aaattgccct gcggaattta aacctccaaa aaaagcagct gctttcagag
     1621 gaggaaaaga agaatttatc agcatctacg gtattaactg cccaagaatc attttccggt
     1681 tcacttgggc atttacagaa taggaacaac agttgtgatt ccagaggaca gactactaaa
     1741 gccaggtttt tatatggaga gaacatgcca ccacaagatg cagaaatagg ttaccggaat
     1801 tcattgagac aaactaacaa aactaaacag tcatgcccat ttggaagagt cccagttaac
     1861 cttctaaata gcccagattg tgatgtgaag acggacgatt cagttgtacc ttgttttatg
     1921 aaaagacaaa cctctagatc agaatgccga gatttggttg tgcctggatc taaaccaagt
     1981 ggaaatgatt cctgtgaatt aagaaattta aagtctgttc aaaatagtca tttcaaggaa
     2041 cctctggtgt cagatgaaaa gagttctgaa cttattatta ctgattcaat aaccctgaag
     2101 aataaaacgg aatcaagtct tctagctaaa ttagaagaaa ctaaagagta tcaagaacca
     2161 gaggttccag agagtaacca gaaacagtgg caatctaaga gaaagtcaga gtgtattaac
     2221 cagaatcctg ctgcatcttc aaatcactgg cagattccgg agttagcccg aaaagttaat
     2281 acagagcaga aacataccac ttttgagcaa cctgtctttt cagtttcaaa acagtcacca
     2341 ccaatatcaa catctaaatg gtttgaccca aaatctattt gtaagacacc aagcagcaat
     2401 accttggatg attacatgag ctgttttaga actccagttg taaagaatga ctttccacct
     2461 gcttgtcagt tgtcaacacc ttatggccaa cctgcctgtt tccagcagca acagcatcaa
     2521 atacttgcca ctccacttca aaatttacag gttttagcat cttcttcagc aaatgaatgc
     2581 atttcggtta aaggaagaat ttattccata ttaaagcaga taggaagtgg aggttcaagc
     2641 aaggtatttc aggtgttaaa tgaaaagaaa cagatatatg ctataaaata tgtgaactta
     2701 gaagaagcag ataaccaaac tcttgatagt taccggaacg aaatagctta tttgaataaa
     2761 ctacaacaac acagtgataa gatcatccga ctttatgatt atgaaatcac ggaccagtac
     2821 atctacatgg taatggagtg tggaaatatt gatcttaata gttggcttaa aaagaaaaaa
     2881 tccattgatc catgggaacg caagagttac tggaaaaata tgttagaggc agttcacaca
     2941 atccatcaac atggcattgt tcacagtgat cttaaaccag ctaactttct gatagttgat
     3001 ggaatgctaa agctaattga ttttgggatt gcaaaccaaa tgcaaccaga tacaacaagt
     3061 gttgttaaag attctcaggt tggcacagtt aattatatgc caccagaagc aatcaaagat
     3121 atgtcttcct ccagagagaa tgggaaatct aagtcaaaga taagccccaa aagtgatgtt
     3181 tggtccttag gatgtatttt gtactatatg acttacggga aaacaccatt tcagcagata
     3241 attaatcaga tttctaaatt acatgccata attgatccta atcatgaaat tgaatttccc
     3301 gatattccag agaaagatct tcaagatgtg ttaaagtgtt gtttaaaaag ggacccaaaa
     3361 cagaggatat ccattcctga gctcctggct catccatatg ttcaaattca aactcatcca
     3421 gttaaccaaa tggccaaggg aaccactgaa gaaatgaaat atgttctggg ccaacttgtt
     3481 ggtctgaatt ctcctaactc cattttgaaa gctgctaaaa ctttatatga acactatagt
     3541 ggtggtgaaa gtcataattc ttcatcctcc aagacttttg aaaaaaaaag gggaaaaaaa
     3601 tgagtcgagc atgcggtacc aagcttgtcg agaagtacta gaggatcata atcagccata
     3661 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3721 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3781 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3841 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc
     3901 ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt
     3961 ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat
     4021 aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc
     4081 ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa
     4141 ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt catctcttcg
     4201 ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa tggcgaatgg
     4261 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4321 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4381 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4441 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4501 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
     4561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4621 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4681 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     4741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     6661 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     6721 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     6781 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     6841 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     6901 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     6961 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     7021 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     7081 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     7141 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     7201 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     7261 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     7321 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     7381 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     7441 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     7501 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     7561 ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc
     7621 gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg
     7681 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     7741 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt
     7801 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc
     7861 gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag
     7921 gcattggcgg ccttgctgtt cttctacg
//
