LOCUS       sequence-221175-        7491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for gateway cloning containing human PALI1 coding 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, 
            Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, 
            Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel 
            G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, 
            Margueron R, Koseki H, Bracken AP
  TITLE     A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL 
            Genes Balance PRC2 Subtype Activities.
  JOURNAL   Mol Cell. 2018 May 3;70(3):408-421.e8. doi: 
            10.1016/j.molcel.2018.03.005. Epub 2018 Apr 5.
  PUBMED    29628311
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03.005"; journalName: "Mol Cell"; date: 
            "2018-05-3- 3"; volume: "70"; issue: "3"; pages: "408-421.e8"
COMMENT     Sequence Label: pCR8 PALI1
FEATURES             Location/Qualifiers
     source          1..7491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5370..5469)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5486..5505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5487..5505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5510..5526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5510..5526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5957..6748
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6841..7429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7330..7349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttatgcagcg aatgatccaa caatttgctg ctgaatatac
      721 ctcaaaaaat agctctactc aggaccccag ccagcccaat agcacaaaga accaaagcct
      781 gccgaaagca tctccagtca ccacctctcc cacggctgca actactcaga accctgtgct
      841 cagcaaactt ctcatggctg accaagactc acctctggac cttactgtca gaaagtctca
      901 gtcagaacct agcgaacaag acggtgtact tgatctgtcc actaagaaaa gtccatgtgc
      961 tggcagcact tccctgagcc actctccagg ctgctccagt actcaaggga acggtgagaa
     1021 ctcaacagag gcaaaagcag tagattctaa caatcagtcg aagtccccac tggagaaatt
     1081 tatggtcaaa ctgtgtactc atcatcaaaa gcaattcatt cgtgttctga acgacctgta
     1141 cactgaatct caaccaggca ctgaggacct gcagccttct gattcgggag caatggatgt
     1201 atccacttgc aatgctggct gtgcccagct cagcaccaaa cataaggaaa aagatgctct
     1261 gtgtctcgat atgaagtctt ctgcttctgt agatttgttc gtagactcgt cagactctca
     1321 cagccctcta cacttgacgg aacagacccc gaagaagcct cctcctgaga taaaccctgt
     1381 agatggaaga gagaatgcct tgactgttgt ccagaaagat tcctctgaac ttccaaccac
     1441 taaatcgaat tctattaata gcagttcagt ggatagtttc actccgggat acctcactgc
     1501 atctaattgt tcctcagtga acttccacca catccctaaa atcttggagg ggcagaccac
     1561 tggacaagag caagacacaa atgtgaacat atgtgaggat ggtaaagacc atatgcagag
     1621 ttcagcttta gtagaaagtc taattacagt aaaaatggca gctgagaata gtgaggaagg
     1681 caatacctgt attattcctc aaagaaattt gttcaaagct ttatcagaag aggcttggaa
     1741 ctcagggttt atggggaact catctagaac tgctgacaaa gagaatactt tacagtgtcc
     1801 aaaaacacct ttgcgccagg atttagaggc aaatgaacaa gatgcaaggc caaagcaaga
     1861 gaaccatctt cactctctgg gaagaaataa ggtgggttac catttacatc ccagtgataa
     1921 gggccagttt gatcattcca aagatggttg gttaggcccc ggccctatgc cagctgtaca
     1981 caaagcggca aatggacact caagaaccaa gatgatatca acctccatca agacagctcg
     2041 gaaaagtaaa agggcatcag ggctgaggat aaatgattat gataaccagt gtgatgttgt
     2101 ttatatcagt caaccaataa cagaatgcca ctttgagaat caaaaatcaa tattatcttc
     2161 tcggaaaaca gccagaaaga gtactcgagg atactttttc aatggtgact gttgtgagct
     2221 gccaactgtt cgtacactgg ccagaaattt acactcccag gaaaaagcaa gctgctcagc
     2281 attggcatca gaggcagttt tcactcctaa gcagaccctt acaattccag cccctagaca
     2341 tacagtagat gtgcagcttc ccagagaaga caaccctgaa gaacctagca aggaaatcac
     2401 ctctcacgag gaaggaggtg gagacgtttc acctcgaaaa gaacctcaag agcctgaggt
     2461 ttgccccaca aagattaagc cgaacctgag cagctcccct aggtcagagg aaacgacagc
     2521 ctccagcctg gtgtggcctc tccctgctca ccttcctgaa gaggacctgc cagaaggtgg
     2581 ctccacagtc tcagctccca cagcaagtgg gatgtcttct cctgaacaca accaaccacc
     2641 agttgcactg ttggatacgg aggagatgag tgtaccccag gactgtcacc tccttccctc
     2701 cactgaaagc ttttccgggg gagtcagtga agatgtcatt tctaggcctc attctcctcc
     2761 tgaaatagtc agtagagaag aaagtcctca gtgctcagaa aatcagagtt ccccaatggg
     2821 cttggagccc cccatgagtc tgggaaaggc tgaggacaac caaagcatca gtgctgaggt
     2881 tgagtctgga gacacccagg agctaaatgt cgacccactc ttgaaggaaa gcagcacttt
     2941 tactgatgaa aaccccagtg aaactgagga aagtgaggca gcaggtggta taggaaaatt
     3001 agagggagag gacggtgatg taaaatgcct gtcagaaaaa gacacgtatg atacaagcat
     3061 tgactcactc gaagagaatt tggacaagaa gaaaaaaggt aaaaaattcc ctgaggcctc
     3121 tgataggtgc ctaagaagtc aactttcgga ttcttcctct gctgacagat gcctaagaaa
     3181 tcagagttca gattcttcct cagcttgtct tgaaatcaaa gttcctaaaa atcctagtgc
     3241 aaaacgttca aaaaaagaag ggcaccctgg tgggacaaca cctaagggcc ttctacctga
     3301 cagtttccac acggaaactc tggaggacac agaaaagcca agtgtcaatg aacgcccctc
     3361 tgagaaagat gctgagcagg agggcgaagg cggggggatc atcaccaggc agactttgaa
     3421 aaacatgctg gacaaagaag tcaaggagtt acgaggagag attttcccca gcagggaccc
     3481 cataaccaca gctggacagc cactgcctgg agagagattg gaaatctatg ttcagtctaa
     3541 aatggatgag aagaatgctc atatcccctc agaaagtatt gcttgtaaga gggacccaga
     3601 acaggcaaaa gaagagccag ggcatattcc cacacagcat gtggaggagg ctgtgaatga
     3661 ggtagacaac gaaaacaccc agcagaaaga tgatgagagt gatgccccat gcagctctct
     3721 tgggttgtcg agtagtggaa gtggtgatgc tgctagggca ccaaaatcgg tgccaaggcc
     3781 taaaagattg acctcttcaa cctacaacct aagacacgct cattctctgg gctccttgga
     3841 tgcttcaaaa gtgacttcag aaaaggaagc tgcacaagta aaccccataa tgccaaagga
     3901 aaatggagct tcagagagtg gagaccccct agatgaggac gatgttgaca ccgtggtaga
     3961 tgaacagcca aagtttatgg aatggtgtgc tgaggaggag aaccaagagc tcatcgccaa
     4021 cttcaatgcc cagtacatga aagttcagaa gggctggatc cagttggaga aagaaggaca
     4081 gccaacacca agagcaagga acaaatcaga taaactgaaa gagatttgga aaagcaagaa
     4141 aaggtcacgg aaatgtagga gttcattgga gagtcagaag tgttctcctg ttcagatgct
     4201 ctttatgaca aactttaaat tatctaatgt ttgtaaatgg ttcttagaga caactgaaac
     4261 ccggtctcta gtcattgtga agaagctcaa tactcgcctt ccaggagacg ttccccctgt
     4321 caagcatcct cttcagaaat acgctccttc cagcctatat cccagttcac tacaggctga
     4381 gcgcttgaaa aagcacttga agaaatttcc tggagctacc cctgctaaga ataattggaa
     4441 aatgcagaag ctctgggcca aatttcgaga gaatcctgat caagtggagc cagaagatgg
     4501 cagtgatgtc agccccggcc ctaattctga agacagcata gaggaagtca aggaagatag
     4561 aaacagtcat cctccagcaa acctgcccac tccagccagt acccggattc ttagaaaata
     4621 ttccaatatt cgaggaaagc tcagagccca gcaacgttta atcaagaatg agaaaatgga
     4681 atgcccagat gctctggctg tggaaagtaa gccaagtcgt aagagcgtat gcatcaaccc
     4741 tctgatgtcc cccaagcttg ccctgcaagt ggatgcagat gggtttcctg ttaagcccaa
     4801 gagtactgaa ggaatgaagg gaaggaaggg gaagcaggtg tctgaaatct tgcctaaagc
     4861 agaagttcag agtaaacgca agagaacaga aggcagcagc cctccagata gtaagaacaa
     4921 ggggcctacg gtgaaagcca gcaaagaaaa gcatgctgat ggagccacca aaacccctgc
     4981 tgccaagagg ccagctgcaa gggacagaag cagccaaccc cccaaaaaga cgtctttgaa
     5041 agagaataaa gtgaagatcc ctaaaaagtc cgctgggaag agctgccctc cctccaggaa
     5101 agaaaaagag aatacaaaca aaaggccttc ccagtctatt gcctcggaaa cactgacgaa
     5161 acctgcaaaa cagaaggggg ccggtgaatc ctcttcaagg cctcagaaag ccacgaatag
     5221 gaagcagagt agtggaaaga ctcgggccag accctcaacg aaaaccccag agagcagtgc
     5281 agctcagaga aagcgaaagc tgaaggcaaa gctggactgt tcgcacagca aacggaggcg
     5341 gctggatgca aagtgaaagg gcgaattcga cccagctttc ttgtacaaag ttggcattat
     5401 aaaaaataat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     5461 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     5521 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     5581 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     5641 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     5701 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     5761 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     5821 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     5881 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     5941 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     6001 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     6061 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     6121 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     6181 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     6241 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     6301 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     6361 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     6421 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     6481 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     6541 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     6601 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     6661 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     6721 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     6781 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6841 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6901 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6961 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7321 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7381 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7441 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
