LOCUS       sequence-221179-        6558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for gateway cloning containing human JARID2 coding 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, 
            Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, 
            Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel 
            G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, 
            Margueron R, Koseki H, Bracken AP
  TITLE     A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL 
            Genes Balance PRC2 Subtype Activities.
  JOURNAL   Mol Cell. 2018 May 3;70(3):408-421.e8. doi: 
            10.1016/j.molcel.2018.03.005. Epub 2018 Apr 5.
  PUBMED    29628311
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.03.005"; journalName: "Mol Cell"; date: 
            "2018-05-3- 3"; volume: "70"; issue: "3"; pages: "408-421.e8"
COMMENT     Sequence Label: pCR8 JARID2
FEATURES             Location/Qualifiers
     source          1..6558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             1939..1947
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(4437..4536)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4553..4572)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4554..4572)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4577..4593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4577..4593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5024..5815
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5908..6496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6397..6416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gaattcgccc ttatgagcaa ggaaagaccc aagaggaata tcattcagaa
      721 gaaatacgat gacagtgatg ggattccgtg gtcagaagaa cgggtggtac gtaaagtcct
      781 ttatttgtct ctgaaggagt tcaagaattc ccagaagagg cagcatgcgg aaggcattgc
      841 tgggagcctg aaaactgtga atgggctcct tggtaatgac cagtctaagg gattaggacc
      901 agcatcagaa cagtcagaga atgaaaagga cgatgcatcc caagtgtcct ccactagcaa
      961 cgatgttagt tcttcagatt ttgaagaagg gccgtcgagg aaaaggccca ggctgcaagc
     1021 acaaaggaag tttgctcagt ctcagccgaa tagtcccagc acaactccag taaagatagt
     1081 ggagccattg ctaccccctc cagctactca gatatcagac ctctctaaaa ggaagcctaa
     1141 gacagaagat tttcttacct ttctctgcct tcgaggttct cctgcgctgc ccaacagcat
     1201 ggtgtatttt ggaagctctc aggatgagga ggaagtcgag gaggaagatg atgagacaga
     1261 agacgtcaaa acagccacca acaatgcttc atcttcatgc cagtcgaccc ccaggaaagg
     1321 aaaaacccac aaacatgttc acaacgggca tgttttcaat ggttccagca ggtcaacacg
     1381 ggagaaggaa cctgttcaaa aacacaaaag caaagaggcc actcccgcaa aggagaagca
     1441 cagcgatcac cgggctgaca gccgccggga gcaggcttca gctaaccacc ccgcagcggc
     1501 cccctccacg ggttcctcgg ccaaggggct tgctgccacc catcaccacc cccctctgca
     1561 tcggtcggct caggacttac ggaaacaggt ttctaaggta aacggagtca ctcgaatgtc
     1621 atctctgggt gcaggtgtaa ccagtgccaa aaagatgcgc gaggtcagac cttcaccatc
     1681 caaaactgtg aagtacactg ccacggtgac gaagggggct gtcacataca ccaaagccaa
     1741 gagagaactg gtcaaggaca ccaaacccaa tcaccacaag cccagttccg ctgtcaacca
     1801 cacaatctca gggaaaactg aaagtagcaa tgcaaaaacc cgcaaacagg tgctatccct
     1861 cgggggggcg tccaagtcca ctgggcccgc cgtcaatggc ctcaaggtca gtggcaggtt
     1921 gaacccaaag tcatgcacta aggaggtggg ggggcggcag ctgcgggagg gcctgcagct
     1981 gcgggagggg ctgcggaact ccaagaggag actggaagag gcacaccagg cggagaagcc
     2041 gcagtcgccc cccaagaaga tgaaaggggc ggctggcccc gccgaaggcc ctggcaagaa
     2101 ggccccggcc gagagaggtc tgctgaacgg acacgtgaag aaggaagtgc cggagcgcag
     2161 tctggagagg aatcggccga agcgggccac ggccgggaag agcacgccag gcagacaagc
     2221 acatggcaag gcggacagcg cctcctgtga aaatcgttct acctcgcaac cggagtccgt
     2281 gcacaagccg caggactcgg gcaaggccga gaagggcggc ggcaaggccg ggtgggcggc
     2341 catggacgag atccccgtcc tcaggccctc cgccaaggag ttccacgatc cgctcatcta
     2401 catcgagtcg gtccgcgctc aggtggagaa gttcgggatg tgcagggtga tcccccctcc
     2461 ggactggcgg cccgagtgca agctcaacga tgagatgcgg tttgtcacgc agattcagca
     2521 catccacaag ctgggccggc gctggggccc caacgtgcag cggctggcct gcatcaagaa
     2581 gcacctcaaa tctcagggca tcaccatgga cgagctcccg ctcatagggg gctgtgagct
     2641 cgacctggcc tgctttttcc ggctgattaa tgagatgggc ggcatgcagc aagtgactga
     2701 cctcaaaaaa tggaacaaac tagcagacat gctgcgcatc cccagaactg cccaggaccg
     2761 gctggccaag ctgcaggagg cctactgcca gtacctactc tcctacgact ccctgtcccc
     2821 agaggagcac cggcggctgg agaaggaggt gctgatggag aaggagatcc tggagaagcg
     2881 caaggggccg ctggaaggcc acacagagaa cgaccaccac aagttccacc ctctgccccg
     2941 cttcgagccc aagaatgggc tcatccacgg cgtggccccc aggaacggct tccgcagcaa
     3001 gctcaaggag gtgggccagg cccagttgaa gactggccgg cggcgactct tcgctcagga
     3061 aaaagaagtg gtcaaggaag aggaggagga caaaggcgtc ctcaatgact tccacaagtg
     3121 catctataag ggaaggtctg tttctctaac aactttttat cgaacagcga ggaatatcat
     3181 gagcatgtgt ttcagcaagg agcctgcccc agccgaaatc gagcaagagt actggaggct
     3241 agtggaagag aaggactgcc acgtggcagt gcactgcggc aaggtggaca ccaacactca
     3301 cggcagtgga ttcccagtag gaaaatcaga acccttttcg aggcatggat ggaacctcac
     3361 cgtcctcccc aataacacag ggtccatcct gcgtcacctc ggtgctgtgc ctggagtgac
     3421 tattccctgg ctaaatattg gcatggtctt ttctacctca tgctggtctc gagaccaaaa
     3481 tcaccttcca tacattgact acttacacac tggtgctgac tgcatttggt attgcattcc
     3541 tgctgaggag gagaacaagc tggaagatgt ggtccacacc ctgctgcaag ccaatggcac
     3601 cccagggctg cagatgctgg aaagcaacgt catgatctcc ccggaggtgc tgtgcaaaga
     3661 ggggatcaag gtgcacagga ccgtgcagca gagtggccag tttgtcgtct gcttcccggg
     3721 atcctttgtg tccaaagtgt gctgtgggta cagcgtgtct gaaaccgtgc actttgctac
     3781 cacccagtgg acaagtatgg gctttgagac cgccaaggaa atgaagcgtc gccatatagc
     3841 taagccattc tccatggaga agttactcta ccagattgca caagcagaag caaaaaaaga
     3901 aaacggtccc actctcagta ccatctcagc cctcctggat gagctcaggg atacagagct
     3961 gcggcagcgc aggcagctgt tcgaggctgg cctccactcc tccgcacgct atggcagcca
     4021 cgatggcagc agcacggtgg cggacgggaa gaaaaagcct cgaaagtggc tgcagttgga
     4081 gacgtcagag aggaggtgtc agatctgcca gcacctgtgc tacctgtcca tggtggtaca
     4141 agagaacgaa aacgtcgtgt tctgtctgga gtgtgctctg cgccacgtgg agaaacagaa
     4201 gtcctgccga gggctgaagt tgatgtaccg ctacgatgag gaacagatta tcagtctggt
     4261 caatcagatc tgcggcaaag tgtctggtaa aaacggcagc attgagaact gtctcagtaa
     4321 acccacacca aaaagaggtc cccgcaagag agcgacagtg gacgtgcccc cctcccgtct
     4381 gtcagcctcc agttcatcca aaagtgcttc gagctcatca tgaaagggcg aattcgaccc
     4441 agctttcttg tacaaagttg gcattataaa aaataattgc tcatcaattt gttgcaacga
     4501 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     4561 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     4621 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     4681 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     4741 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     4801 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     4861 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     4921 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     4981 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     5041 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     5101 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     5161 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     5221 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     5281 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     5341 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     5401 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     5461 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     5521 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     5581 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     5641 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     5701 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     5761 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     5821 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     5881 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5941 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6001 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6061 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6121 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6181 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6241 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6301 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6361 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6421 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6481 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6541 gccttttgct cacatgtt
//
