LOCUS       sequence-221187-       12254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette 
            into the mouse TIGRE locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12254)
  AUTHORS   Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, 
            Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts 
            LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz 
            GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA,
            Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT,
            Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, 
            Waters J, Li L, Tasic B, Zeng H
  TITLE     A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced 
            Brain-Cell-Type Targeting and Functionality.
  JOURNAL   Cell. 2018 Jul 12;174(2):465-480.e22. doi: 
            10.1016/j.cell.2018.06.035.
  PUBMED    30007418
REFERENCE   2  (bases 1 to 12254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06"; journalName: "Cell"; date: "2018-07-12- 
            12"; volume: "174"; issue: "2"; pages: "465-480.e22"
COMMENT     Sequence Label: Ai133 (TITL-ssAPEX2tm) targeting vector
FEATURES             Location/Qualifiers
     source          1..12254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        2993..3307
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3001..3019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3037..3055
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3072..3090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3108..3126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3144..3162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3179..3197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3215..3233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3280..3304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3317..3350)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3428..3476
                     /note="synthetic polyadenylation signal"
     polyA_signal    3485..3961
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3684..3703)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     polyA_signal    3973..4434
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(4461..4494)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4590..4613
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5367..5393
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    5496..6084
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5549..5569)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5967..5978)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6109..6126)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6115..6322
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8826..8859
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        8886..9385
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8921..8944)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9318..9337
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(10033..10049)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10033..10049)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10046..10068)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10057..10073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10081..10087,10088..10105,10106..10111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10126..10147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10264..10281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10435..11023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10515..10534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11194..11985,11986..12054))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11817..11836
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12055..12159)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12227..12245
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgttttat
      481 gccgcggtta ccgtgaagat atcttaatta agaattcgag ctcgctagag ccccatcctc
      541 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
      601 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
      661 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
      721 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
      781 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
      841 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
      901 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
      961 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     1021 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     1081 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     1141 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     1201 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     1261 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     1321 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     1381 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     1441 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     1501 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     1561 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     1621 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     1681 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt cccgagctcg
     1741 gtaccgcccc atcctcactg actccgtcct ggagttggat gagagataat ggccttacgt
     1801 tgtgccaggg gagggtcggg ctggatttag caagatttac cttctccaaa gagcggtgct
     1861 gcagtggcac agctgcccac ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac
     1921 tgtggggatg tggcactgag ggacatggcc agtgggcacg gtgggtgggt tggggttggt
     1981 cttggggatc ttggagggct tttccagcct tcatgatttg acgattgtat gaacatctac
     2041 atggcaattc tccagctgcc tgtcccagtc ctactgaccc agctgtatct ctccaggcaa
     2101 gctcttccac cccttctgct tgcatccaga caccatcaaa catgcaggct cagacacagg
     2161 gaccagcagt gtctgtggcc tttttgtgct cctctccatg ctgggtttta acttgctctt
     2221 tgtccttcta tcctatcttc ttatccttaa ggctgttctg aacgctgtga cttggagagt
     2281 gtcccagagc cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc
     2341 ccaagatctg cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca
     2401 atgtcacagc aaactccccc ttgataggac aagggggaat ggctttacac tgagacaggg
     2461 gaggtttggg ttggatatga ggaggcagtt tttcccccag agggtggtga cgcactgaac
     2521 aggttgccca aggaggctgt ggatgcccca tccctgcagg cattcaaggc caggctggat
     2581 gtggctctgg gcagcctggg ctgctggttg atgaccctgc acatagcagg gggttggatc
     2641 tggatgagca ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc attctaaatc
     2701 tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc
     2761 agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc
     2821 cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg
     2881 ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg
     2941 ggctgtcccg gtacccgggg atccaccggt atcgattacc tatcacaaat tcgagtttac
     3001 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
     3061 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     3121 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     3181 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     3241 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     3301 gatcgcctat ttaaatataa cttcgtatag catacattat acgaagttat tcgcgagtga
     3361 ataaatgaaa gcttgcagat cccctcagca ccatggctag cggcagcctc ggagtttgaa
     3421 tagatagaat aaaatatctt tattttcatt ccatctgtgt gttggttttt tgtgtgagat
     3481 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
     3541 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
     3601 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
     3661 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
     3721 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc aggctcccga
     3781 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
     3841 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
     3901 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
     3961 tctgcctcag cgctgtaagt ctgcagaaat tgatgatcta ttaaacaata aagatgtcca
     4021 ctaaaatgga agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca
     4081 ttttgaatgg aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct
     4141 gctctttact gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat
     4201 aatttaaaca agcaaaacca aattaagggc cagctcattc ctcccactca tgatctatag
     4261 atctatagat ctctcgtggg atcattgttt ttctcttgat tcccactttg tggttctaag
     4321 tactgtggtt tccaaatgtg tcagtttcat agcctgaaga acgagatcag cagcctctgt
     4381 tccacataca cttcattctc agtattgttt tgccaagttc taattccatc agaaagcttt
     4441 taattaactg aatagctaac ataacttcgt atagcataca ttatacgaag ttatcacgcg
     4501 tgtttgggac gcgtgccacc atgaaatgta aattcctggg tagcttcttt ctgctcttca
     4561 gcctttccgg caaaggggcg gactgcatgg actataaaga cgacgacgat aaaggaaaga
     4621 gctacccaac agtgtccgct gactatcagg atgctgtcga aaaagcaaag aaaaagctga
     4681 gggggtttat cgctgagaag agatgcgcac cactgatgct ccggctggcc ttccactccg
     4741 ctggtacctt tgacaaaggc accaagacag gcggaccctt cggaaccatc aagcaccctg
     4801 cagaactcgc ccattctgct aacaatggcc tggacatcgc cgtgagactg ctcgaacctc
     4861 tcaaggccga gttcccaatt ctgagttacg ctgactttta tcagctggca ggcgtggtcg
     4921 ccgtggaggt cactgggggt cctaaagtgc cattccaccc aggacgagag gacaagcctg
     4981 aaccacctcc agagggacga ctgccagatc ccaccaaagg gagcgaccat ctgagggacg
     5041 tgttcggcaa ggcaatgggc ctcactgacc aggacatcgt ggccctgtcc ggcggacaca
     5101 ccattggagc cgctcataag gaaagaagtg gattcgaggg gccctggacc agcaaccccc
     5161 tgatcttcga caattcctac tttactgagc tgctctctgg agagaaagaa gggctgctcc
     5221 agctgccatc agacaaggct ctgctcagcg accccgtgtt caggccactg gtcgacaaat
     5281 acgcagccga cgaagatgca ttctttgccg attatgctga agcacatcag aagctctctg
     5341 agctgggatt cgcagacgct ctccagctgc cacctctgga gcgcctcacc ctggatgtca
     5401 cagtgggggt cggtgcagga ggactcctct tggtgctctt ggtggcgctt tttattttct
     5461 gtatctgaac gcggcgttac gtaaagcttg tcgataatca acctctggat tacaaaattt
     5521 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5581 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5641 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5701 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5761 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5821 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5881 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     5941 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6001 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6061 tctccctttg ggccgcctcc ccgcatcgac accgagcgct gctcgaggcc tcgactgtgc
     6121 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6181 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6241 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6301 acaatagcag gcatgctggg gacacgtggg atcctctagt ctagagcccc atcctcactg
     6361 actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg
     6421 ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac
     6481 ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag
     6541 ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct
     6601 tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc
     6661 tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct
     6721 tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc
     6781 tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc
     6841 ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc
     6901 tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg
     6961 atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc
     7021 ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga
     7081 ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt
     7141 ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg
     7201 ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct
     7261 ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt
     7321 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     7381 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     7441 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     7501 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct ctagagcccc
     7561 atcctcactg actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg
     7621 gagggtcggg ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac
     7681 agctgcccac ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg
     7741 tggcactgag ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc
     7801 ttggagggct tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc
     7861 tccagctgcc tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac
     7921 cccttctgct tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt
     7981 gtctgtggcc tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta
     8041 tcctatcttc ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc
     8101 cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg
     8161 cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc
     8221 aaactccccc ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg
     8281 ttggatatga ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca
     8341 aggaggctgt ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg
     8401 gcagcctggg ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca
     8461 ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag
     8521 cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
     8581 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
     8641 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
     8701 ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct
     8761 ctagaagtcg acggcgcgcc gtaaaagctt ggcatagatc ttattgcaga agccatatgt
     8821 tttaggtgcc agggcgtgcc cttgggctcc ccgggcgcgt ttgggcggcc gcatcgaatt
     8881 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     8941 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     9001 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     9061 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     9121 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     9181 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     9241 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     9301 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     9361 ctcttcctca tctccgggcc tttcgacctg caatcgccgc tagccgccac catgaaaaag
     9421 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     9481 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     9541 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     9601 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     9661 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     9721 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     9781 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     9841 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     9901 actgtgatgg acgacaccgt cagtgtaagt gctagcccct aggcctgaat cgaagttcct
     9961 attcttcaaa aggtatagga acttcgagct cgtcgactgc agaggcctgc atgcaagctt
    10021 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10081 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10141 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10201 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10861 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    11101 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    11161 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    11221 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    11281 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    11341 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    11401 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    11461 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    11521 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    11581 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    11641 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    11701 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    11761 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    11821 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    11881 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    11941 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    12001 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    12061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    12121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    12181 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    12241 gaggcccttt cgtc
//
