LOCUS       sequence-221189-       17326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target a Cre-dependent voltage sensor ASAP2s cassette into the mouse
            TIGRE locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17326)
  AUTHORS   Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, 
            Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts 
            LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz 
            GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA,
            Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT,
            Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, 
            Waters J, Li L, Tasic B, Zeng H
  TITLE     A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced 
            Brain-Cell-Type Targeting and Functionality.
  JOURNAL   Cell. 2018 Jul 12;174(2):465-480.e22. doi: 
            10.1016/j.cell.2018.06.035.
  PUBMED    30007418
REFERENCE   2  (bases 1 to 17326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06"; journalName: "Cell"; date: "2018-07-12- 
            12"; volume: "174"; issue: "2"; pages: "465-480.e22"
COMMENT     Sequence Label: Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector
FEATURES             Location/Qualifiers
     source          1..17326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2959..3040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2984..3003
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    join(3054..3072,3073..3095,3096..3114,3115..3137,3138..3156,
                     3157..3179,3180..3198,3199..3221,3222..3240,3241..3263,
                     3264..3282,3283..3305,3306..3324)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3357..3395
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3392..3416
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3529..3562)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3640..3688
                     /note="synthetic polyadenylation signal"
     polyA_signal    3697..4173
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    4185..4646
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(4673..4706)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6256..6844
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6727..6738)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6875..7082
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        9612..9915
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9917..10194
                     /label=chicken beta-actin promoter
     intron          10196..11213
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     11137..11159
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     11221..11240
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    11301..11334
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    11372..11420
                     /note="synthetic polyadenylation signal"
     polyA_signal    11429..11905
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(11940..11959)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11984..12032
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    12211..12244
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             join(12269..12886,12887..12892,12893..12928,12929..12931,
                     12932..12967,12968..12970,12971..13006,13007..13015)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    13058..13646
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(13529..13540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    13677..13884
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    13898..13931
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        13958..14457
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13993..14016)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     14390..14409
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(15105..15121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15105..15121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15118..15140)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15129..15145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15153..15159,15160..15177,15178..15183))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15198..15219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15336..15353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15507..16095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15587..15606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16266..17057,17058..17126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16889..16908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17127..17231)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     17299..17317
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgtttaat
      481 tcgagctcgc tagagcccca tcctcactga ctccgtcctg gagttggatg agagataatg
      541 gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc ttctccaaag
      601 agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc cctggaggtg
      661 atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg tgggtgggtt
      721 ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga cgattgtatg
      781 aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca gctgtatctc
      841 tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac atgcaggctc
      901 agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc tgggttttaa
      961 cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga acgctgtgac
     1021 ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct gtcctgcact
     1081 tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat gtgtcacccc
     1141 agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg gctttacact
     1201 gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga gggtggtgac
     1261 gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc attcaaggcc
     1321 aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca catagcaggg
     1381 ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat gattctgtca
     1441 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     1501 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     1561 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     1621 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     1681 ttgggggggg gctgtcccga gctcggtacc gccccatcct cactgactcc gtcctggagt
     1741 tggatgagag ataatggcct tacgttgtgc caggggaggg tcgggctgga tttagcaaga
     1801 tttaccttct ccaaagagcg gtgctgcagt ggcacagctg cccacggagg tgggggggtc
     1861 accgtccctg gaggtgatga agaactgtgg ggatgtggca ctgagggaca tggccagtgg
     1921 gcacggtggg tgggttgggg ttggtcttgg ggatcttgga gggcttttcc agccttcatg
     1981 atttgacgat tgtatgaaca tctacatggc aattctccag ctgcctgtcc cagtcctact
     2041 gacccagctg tatctctcca ggcaagctct tccacccctt ctgcttgcat ccagacacca
     2101 tcaaacatgc aggctcagac acagggacca gcagtgtctg tggccttttt gtgctcctct
     2161 ccatgctggg ttttaacttg ctctttgtcc ttctatccta tcttcttatc cttaaggctg
     2221 ttctgaacgc tgtgacttgg agagtgtccc agagccctca acacctgcat gtcccacgtc
     2281 catgctgtcc tgcacttcct tatccccaag atctgcctct ccgtgatgca ctgaattggc
     2341 aaacatgtgt caccccagac caacaatgtc acagcaaact cccccttgat aggacaaggg
     2401 ggaatggctt tacactgaga caggggaggt ttgggttgga tatgaggagg cagtttttcc
     2461 cccagagggt ggtgacgcac tgaacaggtt gcccaaggag gctgtggatg ccccatccct
     2521 gcaggcattc aaggccaggc tggatgtggc tctgggcagc ctgggctgct ggttgatgac
     2581 cctgcacata gcagggggtt ggatctggat gagcactgtg ctcctttgca acccaggccg
     2641 ttctatgatt ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc
     2701 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     2761 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     2821 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     2881 tacatccctg ggggctttgg gggggggctg tcccggtacc cggggatcca ccggtatcga
     2941 ttcggccggc cgacgtcggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3001 tgctttattt gtaaccatta taagctgcaa taaacaagtt tcgagtttac cactccctat
     3061 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     3121 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     3181 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     3241 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     3301 accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta
     3361 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     3421 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     3481 gcggccccga attcgagctc ggtacccggg gatcaaattc aattaaatat aacttcgtat
     3541 agcatacatt atacgaagtt attcgcgagt gaataaatga aagcttgcag atcccctcag
     3601 caccatggct agcggcagcc tcggagtttg aatagataga ataaaatatc tttattttca
     3661 ttccatctgt gtgttggttt tttgtgtgag atctacgggt ggcatccctg tgacccctcc
     3721 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3781 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3841 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3901 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3961 gttcaagcga ttctcctgcc tcaggctccc gagttgttgg gattccaggc atgcatgacc
     4021 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4081 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4141 caggcgtgaa ccactgctcc cttccctgtc cttctgcctc agcgctgtaa gtctgcagaa
     4201 attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact
     4261 ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg
     4321 ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg
     4381 ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg
     4441 gccagctcat tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt
     4501 ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc
     4561 atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt
     4621 tttgccaagt tctaattcca tcagaaagct tttaattaac tgaatagcta acataacttc
     4681 gtatagcata cattatacga agttatcacg cgtatggaga cgactgtgag gtatgaacag
     4741 gggtcagagc tcactaaaac ttcgagctct ccaacagcag atgagcccac gataaagatt
     4801 gatgatggtc gtgatgaggg taatgaacaa gacagctgtt ccaataccat taggagaaaa
     4861 atttccccgt ttgtgatgtc atttggattc agagtatttg gagttgtgct tatcattgta
     4921 gacatcatag tggtgattgt ggatctggcc atcagtgaga agaaaagagg cattagagag
     4981 attcttgaag gtgtttccct ggctatagca ctcttcttcc ttgttgatgt tctcatgaga
     5041 gtgtttgttg aaggcttcaa gaactatttc cggtccaaac tgaatacttt ggatgcagtc
     5101 atagtagtgg gcactctgct aattaatatg acctactcct tctctgacct tgctgccttt
     5161 aacagccata acgtgtatat taccgcggat aaacagaaaa acggcattaa agcgaacttt
     5221 accgtgcgcc ataacgtgga agatggcagc gtgcagctgg cggatcatta tcagcagaac
     5281 accccgattg gcgatggccc ggtgctgctg ccggataacc attatctgag cacccagacc
     5341 gtgctgagca aagatccgaa cgaaaaacgc gatcacatgg tgctgctgga atttgtgacc
     5401 gcagcgggca ttacacacgg catggatgaa ctgtatggcg gcaccggcgg cagcgcgagc
     5461 cagggcgaag aactgtttac cggcgtggtg ccgattctgg tggaactgga tggcgatgtg
     5521 aacggccata aatttagcgt gcgcggcgaa ggcgaaggcg atgcgaccat tggcaaactg
     5581 accctgaaat ttatttgcac caccggcaaa ctaccggtgc cgtggccgac cctggtgacc
     5641 accttaacct atggcgtgca gtgctttagc cgctatccgg atcatatgaa acgccatgat
     5701 ttttttaaaa gcgcgatgcc ggaaggctat gtgcaggaac gcaccattag ctttaaagat
     5761 gatggcaaat ataaaacccg cgcggtggtg aaatttgaag gcgataccct ggtgaaccgc
     5821 attgaactga aaggcaccga ttttaaagaa gatggcaaca ttctggggca taaactggaa
     5881 tataacacag atcagatgcc gcagatggtt actcttcttc gagttctgag aattgttatc
     5941 ttaataagaa tatttcagct ggcttcacag aagaaacaac ttgaagtggt aacccttaag
     6001 ggttcttcag gttcatctgg atcttccacg cgtcagagcc agccgattct gaacaccaaa
     6061 gaaatggcgc cgcagagcaa accgccggaa gaactggaaa tgagcagcat gccgagcccg
     6121 gtggcgccgc tgccggcgcg caccgaaggc gtgattgata tgcgcagcat gagcagcatt
     6181 gatagcttta ttagctgcgc gaccgatttt ccggaagcga cccgcttttg aacgcgttac
     6241 gtaaagcttg tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6301 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6361 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6421 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6481 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6541 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6601 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6661 tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6721 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6781 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6841 ccgcatcgac accgagcgct gctcgaggcc tcgactgtgc cttctagttg ccagccatct
     6901 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6961 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7021 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     7081 gacacgtggg atcctctagt ctagagcccc atcctcactg actccgtcct ggagttggat
     7141 gagagataat ggccttacgt tgtgccaggg gagggtcggg ctggatttag caagatttac
     7201 cttctccaaa gagcggtgct gcagtggcac agctgcccac ggaggtgggg gggtcaccgt
     7261 ccctggaggt gatgaagaac tgtggggatg tggcactgag ggacatggcc agtgggcacg
     7321 gtgggtgggt tggggttggt cttggggatc ttggagggct tttccagcct tcatgatttg
     7381 acgattgtat gaacatctac atggcaattc tccagctgcc tgtcccagtc ctactgaccc
     7441 agctgtatct ctccaggcaa gctcttccac cccttctgct tgcatccaga caccatcaaa
     7501 catgcaggct cagacacagg gaccagcagt gtctgtggcc tttttgtgct cctctccatg
     7561 ctgggtttta acttgctctt tgtccttcta tcctatcttc ttatccttaa ggctgttctg
     7621 aacgctgtga cttggagagt gtcccagagc cctcaacacc tgcatgtccc acgtccatgc
     7681 tgtcctgcac ttccttatcc ccaagatctg cctctccgtg atgcactgaa ttggcaaaca
     7741 tgtgtcaccc cagaccaaca atgtcacagc aaactccccc ttgataggac aagggggaat
     7801 ggctttacac tgagacaggg gaggtttggg ttggatatga ggaggcagtt tttcccccag
     7861 agggtggtga cgcactgaac aggttgccca aggaggctgt ggatgcccca tccctgcagg
     7921 cattcaaggc caggctggat gtggctctgg gcagcctggg ctgctggttg atgaccctgc
     7981 acatagcagg gggttggatc tggatgagca ctgtgctcct ttgcaaccca ggccgttcta
     8041 tgattctgtc attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg
     8101 tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt
     8161 ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac
     8221 cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat
     8281 ccctgggggc tttggggggg ggctgtccct ctagagcccc atcctcactg actccgtcct
     8341 ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg ctggatttag
     8401 caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac ggaggtgggg
     8461 gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag ggacatggcc
     8521 agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct tttccagcct
     8581 tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc tgtcccagtc
     8641 ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct tgcatccaga
     8701 caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc tttttgtgct
     8761 cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc ttatccttaa
     8821 ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc tgcatgtccc
     8881 acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg atgcactgaa
     8941 ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc ttgataggac
     9001 aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga ggaggcagtt
     9061 tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt ggatgcccca
     9121 tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg ctgctggttg
     9181 atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct ttgcaaccca
     9241 ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
     9301 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     9361 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     9421 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     9481 gtaattacat ccctgggggc tttggggggg ggctgtccct ctagaagtcg acggcgcgcc
     9541 ctgcaggaat ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     9601 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     9661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     9721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     9781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     9841 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     9901 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     9961 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
    10021 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
    10081 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
    10141 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcggggagt
    10201 cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
    10261 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
    10321 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
    10381 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
    10441 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
    10501 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
    10561 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
    10621 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
    10681 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
    10741 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
    10801 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
    10861 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
    10921 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
    10981 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
    11041 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
    11101 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
    11161 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
    11221 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgatt tgataccgcg
    11281 ggccccgcgt ggacggtacc ataacttcgt ataaagtatc ctatacgaag ttatccatgg
    11341 ctagcggcag cctcggagtt tgaatagata gaataaaata tctttatttt cattccatct
    11401 gtgtgttggt tttttgtgtg agatctacgg gtggcatccc tgtgacccct ccccagtgcc
    11461 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag
    11521 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt
    11581 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc
    11641 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc
    11701 gattctcctg cctcaggctc ccgagttgtt gggattccag gcatgcatga ccaggctcag
    11761 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa
    11821 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg
    11881 aaccactgct cccttccctg tccttctgcc tcagctaatt gagtaggggg gaggctaact
    11941 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
    12001 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
    12061 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
    12121 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
    12181 ggcaggccgt ggaagttcct attctctaga ataacttcgt ataaagtatc ctatacgaag
    12241 ttatacgcgc gcgtccattt aattcaccat gtctagactg gacaagagca aagtcataaa
    12301 ctctgctctg gaattactca atgaagtcgg tatcgaaggc ctgacgacaa ggaaactcgc
    12361 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
    12421 gctcgatgcc ctggcaatcg agatgctgga caggcatcat acccacttct gccccctgga
    12481 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct gtgctctcct
    12541 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
    12601 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
    12661 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg atcaggagca
    12721 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
    12781 acaagcaatt gagctgttcg accatcaggg agccgaacct gccttccttt tcggcctgga
    12841 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cggccgacgc
    12901 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
    12961 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
    13021 taaggatcaa atggcgcgtt acgtaaagct tgtcgataat caacctctgg attacaaaat
    13081 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
    13141 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
    13201 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
    13261 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
    13321 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
    13381 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
    13441 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
    13501 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
    13561 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
    13621 gatctccctt tgggccgcct ccccgcatcg acaccgagcg ctgctcgagg cctcgactgt
    13681 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    13741 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    13801 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    13861 agacaatagc aggcatgctg gggacacgtg ggatccggtg ccagggcgtg cccttgggct
    13921 ccccgggcgc gtttgggcgg ccgcatcgaa ttctaccggg taggggaggc gcttttccca
    13981 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
    14041 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
    14101 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
    14161 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
    14221 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
    14281 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
    14341 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
    14401 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
    14461 tgcaatcgcc gctagccgcc accatgaaaa agcctgaact caccgcgacg tctgtcgaga
    14521 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
    14581 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
    14641 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
    14701 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
    14761 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
    14821 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
    14881 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
    14941 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgtaa
    15001 gtgctagccc ctaggcctga atcgaagttc ctattcttca aaaggtatag gaacttcgag
    15061 ctcgtcgact gcagaggcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct
    15121 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    15181 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    15241 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    15301 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    15361 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    15421 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    15481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    15541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    15601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    15661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    15721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    15781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    15841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    15901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    15961 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    16021 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    16081 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    16141 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    16201 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    16261 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    16321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    16381 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    16441 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    16501 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    16561 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    16621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    16681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    16741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    16801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    16861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    16921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    16981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    17041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    17101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    17161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    17221 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    17281 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
