LOCUS       sequence-221192-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP tethered to a single repeat of the Gcn5 consensus motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X1
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     149..165
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     149..165
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(215..243)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttgctgtctt gctatcnngt ataaatagac ctgcaattat taatcttttg tttcctcgtc
       61 attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca agctatacca
      121 agcatacaat caactccaag ctggccgctc tagaactagt ggatcccccg ggctgcagga
      181 attcatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      241 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      301 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      361 acttgtcact actttcactt atggtgttca atgcttttca agatacccag atcatatgaa
      421 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      481 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      541 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      601 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      661 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      721 agaccattat caacnnnata ctccaattgg cgatggccct gt
//
