LOCUS       sequence-221194-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP tethered to a single repeat of the Gcn5 consensus motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X1
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..117
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     135..151
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     136..152
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(222..242)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgttcttata ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta
       61 actccttcct tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata
      121 actaattaca tgactcgagg tcgacggtat cgataagctt ttaaggtctt tttgaagaac
      181 ctttgtatag ttcatccatg ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga
      241 ccatgtggtc tctcttttcg ttgggatctt tcgaaagggc agattgtgtg gacaggtaat
      301 ggttgtctgg taaaaggaca gggccatcgc caattggagt attttgttga taatggtctg
      361 ctagttgaac gcttccatct tcaatgttgt gtctaatttt gaagttaact ttgattccat
      421 tcttttgttt gtctgccatg atgtatacat tgtgtgagtt atagttgtat tccaatttgt
      481 gtccaagaat gtttccatct tctttaaaat caataccttt taactcgatt ctattaacaa
      541 gggtatcacc ttcaaacttg acttcagcac gtgtcttgta gttcccgtca tctttgaaaa
      601 atatagttct ttcctgtaca taaccttcgg gcatggcact cttgaaaaag tcatgccgtt
      661 tcatatgatc tgggtatctt gaaaagcatt gaacaccata agtgaaagta gtgacaagtg
      721 ttggccatgg aacaggtagt tttccagtag tgcaaataaa tttannngta agttttccgt
      781 atgttgcatc accttcaccc tctccactga c
//
