LOCUS       sequence-221197-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP tethered to two repeats of the Gcn5 consensus motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X2
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     493..513
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(601..617)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(602..618)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(636..654)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tgttccatgg ccaacacttg tcactacttt cacttatggt gttcaatgct tttcaagata
       61 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      121 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      181 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      241 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      301 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      361 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      421 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      481 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      541 tgaactatac aaaggttctt caaaaagacc tggatcatct aagaggcctt aaaagcttat
      601 cgataccgtc gacctcgagt catgtaatta gttatgtcac gcttacattc acgccctccc
      661 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
      721 t
//
