LOCUS       sequence-221200-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-X2 where the first consensus motif's lysine is mutated to 
            arginine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X2R1
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..69
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     87..103
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     88..104
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..212)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcaggttgtc taactccttc cttttcggtt agagcggatg tggggggagg gcgtgaatgt
       61 aagcgtgaca taactaatta catgactcga ggtcgacggt atcgataagc ttttaaggcc
      121 tcttagatga tccaggtctt cttgaagaac ctttgtatag ttcatccatg ccatgtgtaa
      181 tcccagcagc tgttacaaac tcaagaagga ccatgtggtc tctcttttcg ttgggatctt
      241 tcgaaagggc agattgtgtg gacaggtaat ggttgtctgg taaaaggaca gggccatcgc
      301 caattggagt attttgttga taatggtctg ctagttgaac gcttccatct tcaatgttgt
      361 gtctaatttt gaagttaact ttgattccat tcttttgttt gtctgccatg atgtatacat
      421 tgtgtgagtt atagttgtat tccaatttgt gtccaagaat gtttccatct tctttaaaat
      481 caataccttt taactcgatt ctattaacaa gggtatcacc ttcaaacttg acttcagcac
      541 gtgtcttgta gttcccgtca tctttgaaaa atatagttct ttcctgtaca taaccttcgg
      601 gcatggcact cttgaaaaag tcatgccgtt tcatatgatc tgggtatctt gaaaagcatt
      661 gaacaccata agtgaaagta gtgacaagtg ttggccatgg aacanntagt tttccagtag
      721 tgcaaataaa tttaagggta agttttccgt atgttgcatc accttcaccc tctccactga
      781 c
//
