LOCUS       sequence-221201-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-X2 where the second consensus motif's lysine is mutated to 
            arginine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X2R2
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..119
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     103..119
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(169..197)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttgtttcct cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat
       61 ttcaagctat accaagcata caatcaactc caagctggcc gctctagaac tagtggatcc
      121 cccgggctgc aggaattcat gagtaaagga gaagaacttt tcactggagt tgtcccaatt
      181 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      241 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      301 gttccatggc caacacttgt cactactttc acttatggtg ttcaatgctt ttcaagatac
      361 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      421 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      481 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      541 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      601 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagntgga
      661 agcgttcaac tagcagncca ttatcaacaa aatactccaa ttggcg
//
