LOCUS       sequence-221205-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic acetylation substrate consisting of GFP fusion with 3 
            repeats of the Gcn5 consensus motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X3
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..71
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(177..193)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(178..194)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      196..443
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(212..230)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(465..483)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(504..520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(504..520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(517..539)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    528..544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(552..558,559..576,577..582))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    597..618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(736..753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
       61 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa aggttcttca
      121 aaaagacctg gatcatctaa gaggcctggc tcatcaaaga gaccataaaa gcttatcgat
      181 accgtcgacc tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca
      241 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
      301 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
      361 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
      421 gacgctcgaa ggctttaatt tgcggccggt accagctttt gttcccttta gtgagggtta
      481 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      541 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      601 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      661 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gactacggtt tgcgtattgg
      721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaaatcga cgctcaagtc
//
