LOCUS       sequence-221206-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic acetylation substrate consisting of GFP fusion with 3 
            repeats of the Gcn5 consensus motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X3
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     114..132
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     150..166
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     151..167
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(273..293)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttgaatataa ataacgttct taatactaac ataactataa aaaaataaat agggacctag
       61 acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa
      121 tgtaagcgtg acataactaa ttacatgact cgaggtcgac ggtatcgata agcttttatg
      181 gtctctttga tgagccaggc ctcttagatg atccaggtct ttttgaagaa cctttgtata
      241 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
      301 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
      361 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
      421 cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt
      481 tgtctgccat gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa
      541 tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac
      601 cttcaaactt gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc
      661 tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgccgt ttcatatgat
      721 ctgggtatct tgaaaagcat tgaacaccat aagtgaaagt agtgacaagt gttggccatg
      781 gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat
      841 caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaatttca
      901 acaaggaatt ggggacaact ccagtgaaaa gttctttc
//
