LOCUS       sequence-221217-        9576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab25a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab25a
FEATURES             Location/Qualifiers
     source          1..9576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1400..1420)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1428..1444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1460..1594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1484..1503)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1538..1557
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2033..2220,2221..2232)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2266..2282)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2266..2282)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2319..2339)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2319..2337)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2358..2374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2371..2393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2382..2398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2406..2412,2413..2430,2431..2436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2451..2472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2589..2606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2760..3348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2840..2859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3519..4310,4311..4379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4142..4161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4380..4484)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4647..4668)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5017..5028,5029..5191)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5564..5580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5600..5620
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggggaca gatttagcct acaactttgt
      781 cttcaaagtg gttttaattg gagaatcagg ggttggaaag agcaacctgc tgtcaaggtt
      841 taccaaaaac gagttcaacc atgacagtcg caccactatt ggcgtggagt tcagcactcg
      901 ctctgttcag gtggacagta tcaccatcaa agcccagatc tgggacactg cgggtctaga
      961 gcgctacagg gccatcacct cagcgtatta cagaggagca gtgggtgcgc tgctggtcta
     1021 tgacatcacc aaacacctga cctatgagag cgtggagcgc tggctgaagg aactgtatga
     1081 tcacgctgat cctcatatcg tggtcatgct ggtgggcaat aaaaatgacc tggcaactgt
     1141 gcgcactgta cccaccgagg aggccaaaga ctttgcagat gccaatggct tactttttat
     1201 ggagacttca gcattagagt cgactaatgt tgaatctgct ttcctcgaag ttctaacagc
     1261 aatacaccag aaagttgcta gtaaagaagt gaccagaggc tcgatcagcg ccgtgactct
     1321 tggacagaca cacggtccgt ccagggaatt ccaggagagc cgaagctgct gcaaaaactc
     1381 ctgaacacac cagaggacac aactttatta tacatagttg ataattcact ggccgtcgtt
     1441 ttacggtacc atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac
     1501 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1561 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1621 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     1681 tggtatggct gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct
     1741 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
     1801 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
     1861 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
     1921 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
     1981 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
     2041 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     2101 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     2161 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     2221 tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta
     2281 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     2341 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2521 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg cattttggtc
     3421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4381 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4441 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4501 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4561 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4621 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4681 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca cgctggctaa
     4741 gaactcatca gcctccccgg tccatctacc cacgtaccaa tgcaccaatt ggccacaatg
     4801 acggctacta catggtgcca ttccttcctc tttataggaa tggagactac ctcctgtcca
     4861 acaatgctct tggatacgag tacgcctacc tgttggaccc aggtcattgc acaacaccag
     4921 aaatgccctc tgatctgcaa aagacgtgaa tatctgttca gacacccata tccactctgt
     4981 tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa
     5041 attttactca agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag
     5101 tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg
     5161 agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt
     5221 taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt
     5281 gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt
     5341 gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct
     5401 agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga
     5461 tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag
     5521 agactccctg gtgtctgaaa cacaggccag atgggccctc gagcaggaaa cagctatgac
     5581 catgattacg ccaagctatc aactttgtat agaaaagttg gcacgcgcag attccgtgtg
     5641 ggcctggtaa tatcttacca ttttaagcta ttttaagatt agggaaaaaa attaaataaa
     5701 actactttaa aattactttc cttaaaaagc aacatattta tttagttttt gtttaactgg
     5761 ctcaagattg taatatttta cttttacatg tttttttaag tacctaaatg tgttcaaaag
     5821 taaagtatgt tattgttttt tttttttaat gttagctaag ctttaaattg taatatatta
     5881 cttttaaaag tgacttttta aaaatgtaaa taaaaaagta actataaatg tgtaatgcat
     5941 gttatttctg tgggttttta attttcatct caactgggct tacaatttta acataataaa
     6001 aatcaaatca aatcaaatca ggcccttatt tgtcacatac actcttgtat actgaaataa
     6061 actaaatgtt gagaaaaatt taaatacacc aaaataacgc ataccttttt tgtaattacc
     6121 taagtaaaac atttagttcc ttttttaagt gactcaagat tgtaatctat taattgtaaa
     6181 agataaaact agtgatcaat ctgacgtgct tctatgcagc ctgtgagatt aattgtgatg
     6241 tttaattatt aaacaattta aatggatcaa acatttcaca tgtgcttatt gtgtccaaca
     6301 aaaccagcag aagcacttaa aaatattgca tgacaacatg agtttttaat gtaaattata
     6361 tatacttcct aaacgaagtt aatcatattt ctaaaataat aatgtcattg aaatatttaa
     6421 ataaatgata agctttagtt gattcaacat aaaattgaaa gcaataagga cttttttttt
     6481 ttacagcggt gaagcaaaag catgtaataa tcatataata taataaataa ataaataaat
     6541 aatggctctt ggtgcaaata tgaacacttt aaataaatat agaataaata tgatcaataa
     6601 gtcataacga tgtgttttat gttgattata atttaaacat gtttctaacg caagttttaa
     6661 aaactgtttt aaagtattat tttaagttat aagatcaatt tgactcaaca ttttgtagtg
     6721 aaaatatgtt gtgtaaaatt aatataatat acactataaa aatgcagggt tccacacaat
     6781 acattaatgt tgtcccaaca caaatcgatt aagttaacaa atatatgtgg attgaacata
     6841 aaaaattaag ttgtcccaat gaaatctcaa gaattgtgtt gtttcagctc attttaaata
     6901 agtagtttta acaagtaaaa aataacatat ttttgagtgt gtatatatat atattacaaa
     6961 atataataat ataaaatgta aaagatataa accatgctac atatatttaa gagctatttt
     7021 gcacattact tttttgcaag ccattttagt gtttttttta caaatatata ttagctaaat
     7081 atacaatatc ttaaatatac aattaatcag acatgcaatt aatgcattta acctacataa
     7141 aaatacatga tcaacatatg ttttatgctg attacaacta aatcaaacgt aatgaaatta
     7201 atttataaaa gcgatttaaa tatgtcaata ttttaagtta aaatcacttg aaacctacta
     7261 taaatgaact taaattaaaa aaatctcctt taattccaat gcaacagtgt tgttttttca
     7321 gctgaggacc cccggtgcag atggttggcc cccggcccgc tattctaagg aagcctttct
     7381 taattttagg gcggcgctgc atgcgctcag atcagattca cgggtggaga gggttatttt
     7441 ctctttttta ttttttcttg cctgctcata tttgggcctg tgtgaacccc aggctccgcc
     7501 tcggccccca ggagccgctc ttatttaaag gggatcctcg ccttcagtct tcgtcccagt
     7561 ggaagcatca gtcttgtgat aacaacttgg tgagtcctac agaccagcgc tcgactctag
     7621 gctttaaatg cattgatttc tctggattag ctaccgggag agttttaact ttgcttatta
     7681 gtcttgtgct cgaactgcta ggtttaaaga gtgttttctc taatgcatgc atgaagcgac
     7741 agctgaaaac cagaaataga gcgtttgtcc tcctgttaaa ctctagcctt tctttggcat
     7801 ttgtcagtcc gagtcttcag ctgccggcag gagattgggt ttcagtaaca cttgctgagc
     7861 acaaactaca cgtgctttct tcagatttaa ttcattatta atcattctag atttaaatga
     7921 tgttcttact cattgctgat tattttagcc ttgcggagca gattaaatga gcacgtgttt
     7981 cattaagatt gatttaaata taattaatat ttcaccactg tagttttaaa attgccttct
     8041 aagtcatggt tgatttttaa taggtatcgg ctctaattag attactattt tggtttttaa
     8101 atgttatttc tatttcattt gtaggctcgt ttttaatgtg attttattag attctagttt
     8161 taaaatcatc tcttagcctt aatgagcaga ctgaatgaac atgtttcatt aagattaact
     8221 tcattataat gaattattta atcattctag tgggttataa atcagctcta tttaaattaa
     8281 ttgtcgtgtt tatttctatt taatttttga ttattttttc agggtcgttt ttatgtgatt
     8341 ttttcccgtt tctaatttta aactaattgt ttgattttta tatgattcga ctctttttaa
     8401 atgaattgtt ttgctttttt tattgagcat atatatatta tacaatttca atcaacaatt
     8461 gttgtgcttt ttaaatgttg tttctatttc atttctaggg tcgattttat tcgattctag
     8521 ttttaaaatc gtgattttta acttctaacc ttactgagca gactgaatga acatgtttca
     8581 tcaagattaa cttcattata gttaattatt gaatcgtgct agtggtttat aaatcagctc
     8641 tatttaaatt aatcatcatc attatttcta tttaattttt attatttctt taggattatt
     8701 tttatgtgat ttttttcacg ttctagtttt aaactcaatg ctcattactg ctcacccttg
     8761 ctgagcagtc tttcgttaag attaaattaa ttaataacca ttctagtttt aaaacagtgt
     8821 cttaaggcat agctgatatt ttacaagttt actatttaat atatctcctt tcttttttta
     8881 gggtcgattt catctgattt gattaatttt tattatcttc actaagcttt ttgttctagg
     8941 ttttattctc agtattatta ctgttatatt tgaactgaat ttctcctcat agtttattca
     9001 ttttatttat ttattttttg tttattcgct attttgtatt ttagttttta attagaaatt
     9061 ttattagaat cgttttgaac tagtttttat gcactctaac attaatatta aatcataact
     9121 attattttta ttataattta tttgtgttta tctttttgtg tagctttcat gtgtttttat
     9181 tttagtttta ttatgtttgc attgaatttt ttggactagt ttatataacg atatacagaa
     9241 aagccatgac aacaaatgtt tttataatac ctattaagta taagctaatc agatttccac
     9301 tattctgttc aaagttaaca ttattagcca ttctgatgga tcatttgaca tttgtcatat
     9361 gtctttatga acactgctct ttcagatgcc acttttaaag ctgtttttta cagtaaatga
     9421 acgtttatta ttatgacatt atttcctaag gcctctagag tacagcagga tgtggcgtgt
     9481 gcattaccgt gtaattatgc tgactgtaaa tgtgcattta tagactgtca gcatgtctga
     9541 ctgtgcgctg ttgtgattgg tcctgcacag accaag
//
