LOCUS       sequence-22122-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Feinstein P, Mombaerts P
  TITLE     A contextual model for axonal sorting into glomeruli in the mouse 
            olfactory system.
  JOURNAL   Cell. 2004 Jun 11. 117(6):817-31.
  PUBMED    15186781
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Jun 11. 117(6):817-31."
COMMENT     Sequence Label: HybridJ-IRES-tauGFP LNL TV
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(263..279)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..280)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(309..329)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(309..327)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(348..364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(348..364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(361..383)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    372..388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(396..402,403..420,421..426))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    442..462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(579..596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agctcccgcg gcgcggccgc tctagaactg gtggatcccc cgggctgcag cccaatgtgg
       61 aattcgccct tcccttaacc tatcctacaa ccaactaata cacatacccc acttcctacc
      121 acaaaaccta aaacacctaa cactacacca cgaccacatc gaacgcatcc ctaactacgc
      181 gttcgcgcac ataaaaccat acctaaaatt cctacaccta tcccacaaca agggcgaatt
      241 ccacagtgga tatcaagctt atcgataccg tcgacctcga gggggggccc ggtacccagc
      301 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
      361 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg gagcataaag
      421 tgtaaagcct ggggtgccta ctgagtgagc taactcacat taattgcgtt gcgctcactg
      481 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      541 gggagaggcg gtttgcgtat tgggcgctct accgcttcct cgctcactga ctcgctgcgc
      601 tcggtcgttc ggctgcggcg agc
//
