LOCUS       sequence-221222-        9594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab28
FEATURES             Location/Qualifiers
     source          1..9594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1418..1438)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1446..1462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1478..1612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1502..1521)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1556..1575
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2051..2238,2239..2250)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2284..2300)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2284..2300)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2337..2357)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2337..2355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2376..2392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2389..2411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2400..2416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2424..2430,2431..2448,2449..2454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2469..2490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2607..2624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2778..3366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2858..2877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3537..4328,4329..4397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4160..4179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4398..4502)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4665..4686)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5035..5046,5047..5209)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5582..5598
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5618..5638
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgtcggac tccgaggagg agatccagga
      781 gcggcagctg aagatagtgc tgctgggaga cggagcgtca gggaagacgt ctctcgccat
      841 ccgcttcgca caagaagctt ttgggaaaca gtataaacag acgatagggc tggacttctt
      901 cctcaagaga atcacactgc caggaaacct gaatgtgacg ctgcaggtct gggacatcgg
      961 tggacagact attggaggaa aaatgctgga caaatacatc tatggagcac agggtgttct
     1021 cctggtgtat gacatcacta actctcagag ttttgagaat cttgaagatt ggctgagcat
     1081 ggtgagaaaa gccaacgagg agtcagacgt acagccggcc atatcactga tcggaaacaa
     1141 gattgatctg gagcacatgc ggacagtgaa gatggagaag catcagcggt tctgtcagga
     1201 aaatggactc gtcagtcagt ttgtgtctgc caagacggga gattctgtgt ttctgtgttt
     1261 tcagaggctg gcagctgaga ttttgggcat caagctgaat aaagcagaga tggagcagtc
     1321 acagcaggtg gtcaaagcag atatcgtgaa ctacagtcaa gagtctgtgg ctcgagccgt
     1381 caaccctccg cgcagctcca tgtgtgtcat ccagtgacaa ctttattata catagttgat
     1441 aattcactgg ccgtcgtttt acggtaccat cgatgatgat ccagacatga taagatacat
     1501 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1561 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1621 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1681 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctgcgaaga
     1741 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
     1801 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
     1861 tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga
     1921 ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa
     1981 ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga
     2041 aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag
     2101 gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt
     2161 tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa
     2221 gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca
     2281 gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     2341 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     2401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3421 cgttaaggca ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3541 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3601 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3661 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3721 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3781 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3841 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3901 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3961 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4021 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4081 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4141 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4201 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4261 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4321 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4381 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4501 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     4561 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     4621 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     4681 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     4741 atggcccacg ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg
     4801 caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg
     4861 gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg ttggacccag
     4921 gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata tctgttcaga
     4981 cacccatatc cactctgttc cacacaggtc agaggtttgt ccaggagttc ttgacagagg
     5041 tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca agtacttagg gaaaatttta
     5101 ctcaattaaa agtaaaagta tctggctaga atcttacttg agtaaaagta aaaaagtact
     5161 ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa gaaaactaga
     5221 gattcttgtt taagctttta atctcaaaaa acattaaatg aaatgcatac aaggttttat
     5281 cctgctttag aactgtttgt atttaattat caaactataa gacagacaat ctaatgccag
     5341 tacacgctac tcaaagttgt aaaacctcag atttaacttc agtagaagct gattctcaaa
     5401 attgttagtg tcaagcctag ctcttttggg gctgaaaagc aatcctgcag tgctgaaaag
     5461 cctctcacag gcagccgatg cgggaagagg tgtattagtc ttgatagaga ggctgcaaat
     5521 agcaggaaac gtgagcagag actccctggt gtctgaaaca caggccagat gggccctcga
     5581 gcaggaaaca gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttggc
     5641 acgcgcagat tccgtgtggg cctggtaata tcttaccatt ttaagctatt ttaagattag
     5701 ggaaaaaaat taaataaaac tactttaaaa ttactttcct taaaaagcaa catatttatt
     5761 tagtttttgt ttaactggct caagattgta atattttact tttacatgtt tttttaagta
     5821 cctaaatgtg ttcaaaagta aagtatgtta ttgttttttt tttttaatgt tagctaagct
     5881 ttaaattgta atatattact tttaaaagtg actttttaaa aatgtaaata aaaaagtaac
     5941 tataaatgtg taatgcatgt tatttctgtg ggtttttaat tttcatctca actgggctta
     6001 caattttaac ataataaaaa tcaaatcaaa tcaaatcagg cccttatttg tcacatacac
     6061 tcttgtatac tgaaataaac taaatgttga gaaaaattta aatacaccaa aataacgcat
     6121 accttttttg taattaccta agtaaaacat ttagttcctt ttttaagtga ctcaagattg
     6181 taatctatta attgtaaaag ataaaactag tgatcaatct gacgtgcttc tatgcagcct
     6241 gtgagattaa ttgtgatgtt taattattaa acaatttaaa tggatcaaac atttcacatg
     6301 tgcttattgt gtccaacaaa accagcagaa gcacttaaaa atattgcatg acaacatgag
     6361 tttttaatgt aaattatata tacttcctaa acgaagttaa tcatatttct aaaataataa
     6421 tgtcattgaa atatttaaat aaatgataag ctttagttga ttcaacataa aattgaaagc
     6481 aataaggact tttttttttt acagcggtga agcaaaagca tgtaataatc atataatata
     6541 ataaataaat aaataaataa tggctcttgg tgcaaatatg aacactttaa ataaatatag
     6601 aataaatatg atcaataagt cataacgatg tgttttatgt tgattataat ttaaacatgt
     6661 ttctaacgca agttttaaaa actgttttaa agtattattt taagttataa gatcaatttg
     6721 actcaacatt ttgtagtgaa aatatgttgt gtaaaattaa tataatatac actataaaaa
     6781 tgcagggttc cacacaatac attaatgttg tcccaacaca aatcgattaa gttaacaaat
     6841 atatgtggat tgaacataaa aaattaagtt gtcccaatga aatctcaaga attgtgttgt
     6901 ttcagctcat tttaaataag tagttttaac aagtaaaaaa taacatattt ttgagtgtgt
     6961 atatatatat attacaaaat ataataatat aaaatgtaaa agatataaac catgctacat
     7021 atatttaaga gctattttgc acattacttt tttgcaagcc attttagtgt tttttttaca
     7081 aatatatatt agctaaatat acaatatctt aaatatacaa ttaatcagac atgcaattaa
     7141 tgcatttaac ctacataaaa atacatgatc aacatatgtt ttatgctgat tacaactaaa
     7201 tcaaacgtaa tgaaattaat ttataaaagc gatttaaata tgtcaatatt ttaagttaaa
     7261 atcacttgaa acctactata aatgaactta aattaaaaaa atctccttta attccaatgc
     7321 aacagtgttg ttttttcagc tgaggacccc cggtgcagat ggttggcccc cggcccgcta
     7381 ttctaaggaa gcctttctta attttagggc ggcgctgcat gcgctcagat cagattcacg
     7441 ggtggagagg gttattttct cttttttatt ttttcttgcc tgctcatatt tgggcctgtg
     7501 tgaaccccag gctccgcctc ggcccccagg agccgctctt atttaaaggg gatcctcgcc
     7561 ttcagtcttc gtcccagtgg aagcatcagt cttgtgataa caacttggtg agtcctacag
     7621 accagcgctc gactctaggc tttaaatgca ttgatttctc tggattagct accgggagag
     7681 ttttaacttt gcttattagt cttgtgctcg aactgctagg tttaaagagt gttttctcta
     7741 atgcatgcat gaagcgacag ctgaaaacca gaaatagagc gtttgtcctc ctgttaaact
     7801 ctagcctttc tttggcattt gtcagtccga gtcttcagct gccggcagga gattgggttt
     7861 cagtaacact tgctgagcac aaactacacg tgctttcttc agatttaatt cattattaat
     7921 cattctagat ttaaatgatg ttcttactca ttgctgatta ttttagcctt gcggagcaga
     7981 ttaaatgagc acgtgtttca ttaagattga tttaaatata attaatattt caccactgta
     8041 gttttaaaat tgccttctaa gtcatggttg atttttaata ggtatcggct ctaattagat
     8101 tactattttg gtttttaaat gttatttcta tttcatttgt aggctcgttt ttaatgtgat
     8161 tttattagat tctagtttta aaatcatctc ttagccttaa tgagcagact gaatgaacat
     8221 gtttcattaa gattaacttc attataatga attatttaat cattctagtg ggttataaat
     8281 cagctctatt taaattaatt gtcgtgttta tttctattta atttttgatt attttttcag
     8341 ggtcgttttt atgtgatttt ttcccgtttc taattttaaa ctaattgttt gatttttata
     8401 tgattcgact ctttttaaat gaattgtttt gcttttttta ttgagcatat atatattata
     8461 caatttcaat caacaattgt tgtgcttttt aaatgttgtt tctatttcat ttctagggtc
     8521 gattttattc gattctagtt ttaaaatcgt gatttttaac ttctaacctt actgagcaga
     8581 ctgaatgaac atgtttcatc aagattaact tcattatagt taattattga atcgtgctag
     8641 tggtttataa atcagctcta tttaaattaa tcatcatcat tatttctatt taatttttat
     8701 tatttcttta ggattatttt tatgtgattt ttttcacgtt ctagttttaa actcaatgct
     8761 cattactgct cacccttgct gagcagtctt tcgttaagat taaattaatt aataaccatt
     8821 ctagttttaa aacagtgtct taaggcatag ctgatatttt acaagtttac tatttaatat
     8881 atctcctttc tttttttagg gtcgatttca tctgatttga ttaattttta ttatcttcac
     8941 taagcttttt gttctaggtt ttattctcag tattattact gttatatttg aactgaattt
     9001 ctcctcatag tttattcatt ttatttattt attttttgtt tattcgctat tttgtatttt
     9061 agtttttaat tagaaatttt attagaatcg ttttgaacta gtttttatgc actctaacat
     9121 taatattaaa tcataactat tatttttatt ataatttatt tgtgtttatc tttttgtgta
     9181 gctttcatgt gtttttattt tagttttatt atgtttgcat tgaatttttt ggactagttt
     9241 atataacgat atacagaaaa gccatgacaa caaatgtttt tataatacct attaagtata
     9301 agctaatcag atttccacta ttctgttcaa agttaacatt attagccatt ctgatggatc
     9361 atttgacatt tgtcatatgt ctttatgaac actgctcttt cagatgccac ttttaaagct
     9421 gttttttaca gtaaatgaac gtttattatt atgacattat ttcctaaggc ctctagagta
     9481 cagcaggatg tggcgtgtgc attaccgtgt aattatgctg actgtaaatg tgcatttata
     9541 gactgtcagc atgtctgact gtgcgctgtt gtgattggtc ctgcacagac caag
//
