LOCUS       sequence-221227-        9639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab33a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab33a
FEATURES             Location/Qualifiers
     source          1..9639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1463..1483)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1491..1507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1523..1657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1547..1566)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1601..1620
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2096..2283,2284..2295)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2329..2345)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2329..2345)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2382..2402)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2382..2400)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2421..2437)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2434..2456)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2445..2461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2469..2475,2476..2493,2494..2499))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2514..2535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2652..2669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2823..3411)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2903..2922)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3582..4373,4374..4442))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4205..4224
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4443..4547)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4710..4731)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5080..5091,5092..5254)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5627..5643
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5663..5683
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcaaat gaattctcag aaaacagaga
      781 cggaactgcc aactcacgcc atgccaacct cacgtcgtca ctagacctga gcacgtctct
      841 ggatcacagc gtgcagacgc ggatttttaa aatcattgtc atcggggact caaatgtggg
      901 aaagacctgt ttaaccttcc gcttcaccgg cggagccttc ccgtgcaaaa ctgaagccac
      961 catcggcgtg gatttcaggg agaaagcggt ggagatcgag ggcgagaaaa taaaggtaca
     1021 agtatgggac acagcaggcc aggagcgctt tcgaaagagc atggttgagc actactaccg
     1081 caatgtgcat gctgttgttt ttgtgtacga cgtcactaag atggcctcat tccaaaacct
     1141 aaaaacctgg attcaggagt gcaacggtca cggggtgtca tcggcagtgc ctcgtgtttt
     1201 ggtgggcaac aaatgtgatc tagttgacca gatccaggtg ccttccaaca ctgccctcaa
     1261 gtttgccgat gcctataaca tgctactgtt tgaaacatca gccaaagatc ccaaggagag
     1321 ccagaacgtg gactccattt tcatgtgcct ggcatgtcgt ctaaaagccc aaaagtccct
     1381 gatttacaga gatgtggaga gagaagatgg cagagtaagg ctgacgcatc aaccagaccc
     1441 caagagtaac tgcccgtgct gacaacttta ttatacatag ttgataattc actggccgtc
     1501 gttttacggt accatcgatg atgatccaga catgataaga tacattgatg agtttggaca
     1561 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1621 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1681 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     1741 atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc
     1801 tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact
     1861 tcctccattt tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc
     1921 tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct
     1981 gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta
     2041 aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg
     2101 atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt
     2161 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
     2221 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
     2281 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
     2341 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     2401 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     2461 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     2521 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2581 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2641 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2701 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2761 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2821 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2881 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2941 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3001 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3061 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3121 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3181 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3241 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3301 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3361 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3421 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg
     3481 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4441 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4501 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4561 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4621 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4681 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4741 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccacgctggc
     4801 taagaactca tcagcctccc cggtccatct acccacgtac caatgcacca attggccaca
     4861 atgacggcta ctacatggtg ccattccttc ctctttatag gaatggagac tacctcctgt
     4921 ccaacaatgc tcttggatac gagtacgcct acctgttgga cccaggtcat tgcacaacac
     4981 cagaaatgcc ctctgatctg caaaagacgt gaatatctgt tcagacaccc atatccactc
     5041 tgttccacac aggtcagagg tttgtccagg agttcttgac agaggtgtaa aaagtactca
     5101 aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa
     5161 aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac
     5221 ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa ctagagattc ttgtttaagc
     5281 ttttaatctc aaaaaacatt aaatgaaatg catacaaggt tttatcctgc tttagaactg
     5341 tttgtattta attatcaaac tataagacag acaatctaat gccagtacac gctactcaaa
     5401 gttgtaaaac ctcagattta acttcagtag aagctgattc tcaaaattgt tagtgtcaag
     5461 cctagctctt ttggggctga aaagcaatcc tgcagtgctg aaaagcctct cacaggcagc
     5521 cgatgcggga agaggtgtat tagtcttgat agagaggctg caaatagcag gaaacgtgag
     5581 cagagactcc ctggtgtctg aaacacaggc cagatgggcc ctcgagcagg aaacagctat
     5641 gaccatgatt acgccaagct atcaactttg tatagaaaag ttggcacgcg cagattccgt
     5701 gtgggcctgg taatatctta ccattttaag ctattttaag attagggaaa aaaattaaat
     5761 aaaactactt taaaattact ttccttaaaa agcaacatat ttatttagtt tttgtttaac
     5821 tggctcaaga ttgtaatatt ttacttttac atgttttttt aagtacctaa atgtgttcaa
     5881 aagtaaagta tgttattgtt tttttttttt aatgttagct aagctttaaa ttgtaatata
     5941 ttacttttaa aagtgacttt ttaaaaatgt aaataaaaaa gtaactataa atgtgtaatg
     6001 catgttattt ctgtgggttt ttaattttca tctcaactgg gcttacaatt ttaacataat
     6061 aaaaatcaaa tcaaatcaaa tcaggccctt atttgtcaca tacactcttg tatactgaaa
     6121 taaactaaat gttgagaaaa atttaaatac accaaaataa cgcatacctt ttttgtaatt
     6181 acctaagtaa aacatttagt tcctttttta agtgactcaa gattgtaatc tattaattgt
     6241 aaaagataaa actagtgatc aatctgacgt gcttctatgc agcctgtgag attaattgtg
     6301 atgtttaatt attaaacaat ttaaatggat caaacatttc acatgtgctt attgtgtcca
     6361 acaaaaccag cagaagcact taaaaatatt gcatgacaac atgagttttt aatgtaaatt
     6421 atatatactt cctaaacgaa gttaatcata tttctaaaat aataatgtca ttgaaatatt
     6481 taaataaatg ataagcttta gttgattcaa cataaaattg aaagcaataa ggactttttt
     6541 tttttacagc ggtgaagcaa aagcatgtaa taatcatata atataataaa taaataaata
     6601 aataatggct cttggtgcaa atatgaacac tttaaataaa tatagaataa atatgatcaa
     6661 taagtcataa cgatgtgttt tatgttgatt ataatttaaa catgtttcta acgcaagttt
     6721 taaaaactgt tttaaagtat tattttaagt tataagatca atttgactca acattttgta
     6781 gtgaaaatat gttgtgtaaa attaatataa tatacactat aaaaatgcag ggttccacac
     6841 aatacattaa tgttgtccca acacaaatcg attaagttaa caaatatatg tggattgaac
     6901 ataaaaaatt aagttgtccc aatgaaatct caagaattgt gttgtttcag ctcattttaa
     6961 ataagtagtt ttaacaagta aaaaataaca tatttttgag tgtgtatata tatatattac
     7021 aaaatataat aatataaaat gtaaaagata taaaccatgc tacatatatt taagagctat
     7081 tttgcacatt acttttttgc aagccatttt agtgtttttt ttacaaatat atattagcta
     7141 aatatacaat atcttaaata tacaattaat cagacatgca attaatgcat ttaacctaca
     7201 taaaaataca tgatcaacat atgttttatg ctgattacaa ctaaatcaaa cgtaatgaaa
     7261 ttaatttata aaagcgattt aaatatgtca atattttaag ttaaaatcac ttgaaaccta
     7321 ctataaatga acttaaatta aaaaaatctc ctttaattcc aatgcaacag tgttgttttt
     7381 tcagctgagg acccccggtg cagatggttg gcccccggcc cgctattcta aggaagcctt
     7441 tcttaatttt agggcggcgc tgcatgcgct cagatcagat tcacgggtgg agagggttat
     7501 tttctctttt ttattttttc ttgcctgctc atatttgggc ctgtgtgaac cccaggctcc
     7561 gcctcggccc ccaggagccg ctcttattta aaggggatcc tcgccttcag tcttcgtccc
     7621 agtggaagca tcagtcttgt gataacaact tggtgagtcc tacagaccag cgctcgactc
     7681 taggctttaa atgcattgat ttctctggat tagctaccgg gagagtttta actttgctta
     7741 ttagtcttgt gctcgaactg ctaggtttaa agagtgtttt ctctaatgca tgcatgaagc
     7801 gacagctgaa aaccagaaat agagcgtttg tcctcctgtt aaactctagc ctttctttgg
     7861 catttgtcag tccgagtctt cagctgccgg caggagattg ggtttcagta acacttgctg
     7921 agcacaaact acacgtgctt tcttcagatt taattcatta ttaatcattc tagatttaaa
     7981 tgatgttctt actcattgct gattatttta gccttgcgga gcagattaaa tgagcacgtg
     8041 tttcattaag attgatttaa atataattaa tatttcacca ctgtagtttt aaaattgcct
     8101 tctaagtcat ggttgatttt taataggtat cggctctaat tagattacta ttttggtttt
     8161 taaatgttat ttctatttca tttgtaggct cgtttttaat gtgattttat tagattctag
     8221 ttttaaaatc atctcttagc cttaatgagc agactgaatg aacatgtttc attaagatta
     8281 acttcattat aatgaattat ttaatcattc tagtgggtta taaatcagct ctatttaaat
     8341 taattgtcgt gtttatttct atttaatttt tgattatttt ttcagggtcg tttttatgtg
     8401 attttttccc gtttctaatt ttaaactaat tgtttgattt ttatatgatt cgactctttt
     8461 taaatgaatt gttttgcttt ttttattgag catatatata ttatacaatt tcaatcaaca
     8521 attgttgtgc tttttaaatg ttgtttctat ttcatttcta gggtcgattt tattcgattc
     8581 tagttttaaa atcgtgattt ttaacttcta accttactga gcagactgaa tgaacatgtt
     8641 tcatcaagat taacttcatt atagttaatt attgaatcgt gctagtggtt tataaatcag
     8701 ctctatttaa attaatcatc atcattattt ctatttaatt tttattattt ctttaggatt
     8761 atttttatgt gatttttttc acgttctagt tttaaactca atgctcatta ctgctcaccc
     8821 ttgctgagca gtctttcgtt aagattaaat taattaataa ccattctagt tttaaaacag
     8881 tgtcttaagg catagctgat attttacaag tttactattt aatatatctc ctttcttttt
     8941 ttagggtcga tttcatctga tttgattaat ttttattatc ttcactaagc tttttgttct
     9001 aggttttatt ctcagtatta ttactgttat atttgaactg aatttctcct catagtttat
     9061 tcattttatt tatttatttt ttgtttattc gctattttgt attttagttt ttaattagaa
     9121 attttattag aatcgttttg aactagtttt tatgcactct aacattaata ttaaatcata
     9181 actattattt ttattataat ttatttgtgt ttatcttttt gtgtagcttt catgtgtttt
     9241 tattttagtt ttattatgtt tgcattgaat tttttggact agtttatata acgatataca
     9301 gaaaagccat gacaacaaat gtttttataa tacctattaa gtataagcta atcagatttc
     9361 cactattctg ttcaaagtta acattattag ccattctgat ggatcatttg acatttgtca
     9421 tatgtcttta tgaacactgc tctttcagat gccactttta aagctgtttt ttacagtaaa
     9481 tgaacgttta ttattatgac attatttcct aaggcctcta gagtacagca ggatgtggcg
     9541 tgtgcattac cgtgtaatta tgctgactgt aaatgtgcat ttatagactg tcagcatgtc
     9601 tgactgtgcg ctgttgtgat tggtcctgca cagaccaag
//
