LOCUS       sequence-221228-        9657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab33ba.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab33ba
FEATURES             Location/Qualifiers
     source          1..9657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1481..1501)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1509..1525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1541..1675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1565..1584)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1619..1638
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2114..2301,2302..2313)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2347..2363)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2347..2363)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2400..2420)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2400..2418)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2439..2455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2452..2474)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2463..2479
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2487..2493,2494..2511,2512..2517))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2532..2553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2670..2687)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2841..3429)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2921..2940)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3600..4391,4392..4460))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4223..4242
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4461..4565)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4728..4749)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5098..5109,5110..5272)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5645..5661
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5681..5701
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcagat atcgagtcct cttttgagtt
      781 cagctcttct ctgaccagct cgtcactgcc tcctcctcgg acacgcatct ttaaaatcat
      841 cgtgattgga gactcagggg ttggcaagac ttgcctcacc tatagattct gtgcaggcaa
      901 gtttccagac aagactgagg ccactattgg ggtggatttc cgagagaagg ttattgagat
      961 agatggcgag aaaatcaagg tccagttgtg ggacacggca ggccaggagc gtttccgtaa
     1021 gagcatggtg cagcactact accgcaacgt ccatgctgtg gtgtttgtct atgacgtcac
     1081 caatgccgcc agcttccgaa gcctcccagc gtggattgaa gagtgtcgcc aacatgcact
     1141 cggtcaggag gtgccaagaa tccttgtcgg caacaaatgc gacctacgtc acgccgctca
     1201 agtaagcaca gatgttgcac agcagtttgc agatacacac tcaatgcctc tttttgagac
     1261 ctcggccaaa aacccctacg ggaatgaaga cggcactcag aataatagtg accacgtgga
     1321 ggccattttt atgacagtgg cgcacaagct gaaatcccaa aagccgctgg ttttaagcca
     1381 gccaccttgc ggatatggag atactgtcac tcttaggagg caagaccagg aggacggggg
     1441 aaactggggc tgcggttgtt ggagaagcta atgaagtgca caactttatt atacatagtt
     1501 gataattcac tggccgtcgt tttacggtac catcgatgat gatccagaca tgataagata
     1561 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1621 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     1681 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     1741 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc agatctgcga
     1801 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
     1861 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
     1921 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
     1981 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
     2041 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
     2101 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
     2161 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
     2221 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
     2281 caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc
     2341 ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
     2401 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     2461 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2521 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2581 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2641 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2701 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2761 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2821 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2881 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2941 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3001 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3061 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3121 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3181 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3241 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3301 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3361 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3421 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3481 tcacgttaag gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3541 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3601 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3661 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3721 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3781 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3841 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3901 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3961 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4021 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4081 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4141 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4201 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4261 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4321 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4381 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4441 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4501 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4561 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     4621 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     4681 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     4741 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     4801 gcgatggccc acgctggcta agaactcatc agcctccccg gtccatctac ccacgtacca
     4861 atgcaccaat tggccacaat gacggctact acatggtgcc attccttcct ctttatagga
     4921 atggagacta cctcctgtcc aacaatgctc ttggatacga gtacgcctac ctgttggacc
     4981 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
     5041 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
     5101 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
     5161 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
     5221 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
     5281 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     5341 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     5401 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     5461 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     5521 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     5581 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccct
     5641 cgagcaggaa acagctatga ccatgattac gccaagctat caactttgta tagaaaagtt
     5701 ggcacgcgca gattccgtgt gggcctggta atatcttacc attttaagct attttaagat
     5761 tagggaaaaa aattaaataa aactacttta aaattacttt ccttaaaaag caacatattt
     5821 atttagtttt tgtttaactg gctcaagatt gtaatatttt acttttacat gtttttttaa
     5881 gtacctaaat gtgttcaaaa gtaaagtatg ttattgtttt ttttttttaa tgttagctaa
     5941 gctttaaatt gtaatatatt acttttaaaa gtgacttttt aaaaatgtaa ataaaaaagt
     6001 aactataaat gtgtaatgca tgttatttct gtgggttttt aattttcatc tcaactgggc
     6061 ttacaatttt aacataataa aaatcaaatc aaatcaaatc aggcccttat ttgtcacata
     6121 cactcttgta tactgaaata aactaaatgt tgagaaaaat ttaaatacac caaaataacg
     6181 catacctttt ttgtaattac ctaagtaaaa catttagttc cttttttaag tgactcaaga
     6241 ttgtaatcta ttaattgtaa aagataaaac tagtgatcaa tctgacgtgc ttctatgcag
     6301 cctgtgagat taattgtgat gtttaattat taaacaattt aaatggatca aacatttcac
     6361 atgtgcttat tgtgtccaac aaaaccagca gaagcactta aaaatattgc atgacaacat
     6421 gagtttttaa tgtaaattat atatacttcc taaacgaagt taatcatatt tctaaaataa
     6481 taatgtcatt gaaatattta aataaatgat aagctttagt tgattcaaca taaaattgaa
     6541 agcaataagg actttttttt tttacagcgg tgaagcaaaa gcatgtaata atcatataat
     6601 ataataaata aataaataaa taatggctct tggtgcaaat atgaacactt taaataaata
     6661 tagaataaat atgatcaata agtcataacg atgtgtttta tgttgattat aatttaaaca
     6721 tgtttctaac gcaagtttta aaaactgttt taaagtatta ttttaagtta taagatcaat
     6781 ttgactcaac attttgtagt gaaaatatgt tgtgtaaaat taatataata tacactataa
     6841 aaatgcaggg ttccacacaa tacattaatg ttgtcccaac acaaatcgat taagttaaca
     6901 aatatatgtg gattgaacat aaaaaattaa gttgtcccaa tgaaatctca agaattgtgt
     6961 tgtttcagct cattttaaat aagtagtttt aacaagtaaa aaataacata tttttgagtg
     7021 tgtatatata tatattacaa aatataataa tataaaatgt aaaagatata aaccatgcta
     7081 catatattta agagctattt tgcacattac ttttttgcaa gccattttag tgtttttttt
     7141 acaaatatat attagctaaa tatacaatat cttaaatata caattaatca gacatgcaat
     7201 taatgcattt aacctacata aaaatacatg atcaacatat gttttatgct gattacaact
     7261 aaatcaaacg taatgaaatt aatttataaa agcgatttaa atatgtcaat attttaagtt
     7321 aaaatcactt gaaacctact ataaatgaac ttaaattaaa aaaatctcct ttaattccaa
     7381 tgcaacagtg ttgttttttc agctgaggac ccccggtgca gatggttggc ccccggcccg
     7441 ctattctaag gaagcctttc ttaattttag ggcggcgctg catgcgctca gatcagattc
     7501 acgggtggag agggttattt tctctttttt attttttctt gcctgctcat atttgggcct
     7561 gtgtgaaccc caggctccgc ctcggccccc aggagccgct cttatttaaa ggggatcctc
     7621 gccttcagtc ttcgtcccag tggaagcatc agtcttgtga taacaacttg gtgagtccta
     7681 cagaccagcg ctcgactcta ggctttaaat gcattgattt ctctggatta gctaccggga
     7741 gagttttaac tttgcttatt agtcttgtgc tcgaactgct aggtttaaag agtgttttct
     7801 ctaatgcatg catgaagcga cagctgaaaa ccagaaatag agcgtttgtc ctcctgttaa
     7861 actctagcct ttctttggca tttgtcagtc cgagtcttca gctgccggca ggagattggg
     7921 tttcagtaac acttgctgag cacaaactac acgtgctttc ttcagattta attcattatt
     7981 aatcattcta gatttaaatg atgttcttac tcattgctga ttattttagc cttgcggagc
     8041 agattaaatg agcacgtgtt tcattaagat tgatttaaat ataattaata tttcaccact
     8101 gtagttttaa aattgccttc taagtcatgg ttgattttta ataggtatcg gctctaatta
     8161 gattactatt ttggttttta aatgttattt ctatttcatt tgtaggctcg tttttaatgt
     8221 gattttatta gattctagtt ttaaaatcat ctcttagcct taatgagcag actgaatgaa
     8281 catgtttcat taagattaac ttcattataa tgaattattt aatcattcta gtgggttata
     8341 aatcagctct atttaaatta attgtcgtgt ttatttctat ttaatttttg attatttttt
     8401 cagggtcgtt tttatgtgat tttttcccgt ttctaatttt aaactaattg tttgattttt
     8461 atatgattcg actcttttta aatgaattgt tttgcttttt ttattgagca tatatatatt
     8521 atacaatttc aatcaacaat tgttgtgctt tttaaatgtt gtttctattt catttctagg
     8581 gtcgatttta ttcgattcta gttttaaaat cgtgattttt aacttctaac cttactgagc
     8641 agactgaatg aacatgtttc atcaagatta acttcattat agttaattat tgaatcgtgc
     8701 tagtggttta taaatcagct ctatttaaat taatcatcat cattatttct atttaatttt
     8761 tattatttct ttaggattat ttttatgtga tttttttcac gttctagttt taaactcaat
     8821 gctcattact gctcaccctt gctgagcagt ctttcgttaa gattaaatta attaataacc
     8881 attctagttt taaaacagtg tcttaaggca tagctgatat tttacaagtt tactatttaa
     8941 tatatctcct ttcttttttt agggtcgatt tcatctgatt tgattaattt ttattatctt
     9001 cactaagctt tttgttctag gttttattct cagtattatt actgttatat ttgaactgaa
     9061 tttctcctca tagtttattc attttattta tttatttttt gtttattcgc tattttgtat
     9121 tttagttttt aattagaaat tttattagaa tcgttttgaa ctagttttta tgcactctaa
     9181 cattaatatt aaatcataac tattattttt attataattt atttgtgttt atctttttgt
     9241 gtagctttca tgtgttttta ttttagtttt attatgtttg cattgaattt tttggactag
     9301 tttatataac gatatacaga aaagccatga caacaaatgt ttttataata cctattaagt
     9361 ataagctaat cagatttcca ctattctgtt caaagttaac attattagcc attctgatgg
     9421 atcatttgac atttgtcata tgtctttatg aacactgctc tttcagatgc cacttttaaa
     9481 gctgtttttt acagtaaatg aacgtttatt attatgacat tatttcctaa ggcctctaga
     9541 gtacagcagg atgtggcgtg tgcattaccg tgtaattatg ctgactgtaa atgtgcattt
     9601 atagactgtc agcatgtctg actgtgcgct gttgtgattg gtcctgcaca gaccaag
//
