LOCUS       sequence-221229-        9708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab34a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab34a
FEATURES             Location/Qualifiers
     source          1..9708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1532..1552)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1560..1576)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1592..1726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1616..1635)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1670..1689
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2165..2352,2353..2364)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2398..2414)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2398..2414)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2451..2471)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2451..2469)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2490..2506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2503..2525)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2514..2530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2538..2544,2545..2562,2563..2568))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2583..2604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2721..2738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2892..3480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2972..2991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3651..4442,4443..4511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4274..4293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4512..4616)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4779..4800)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5149..5160,5161..5323)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5696..5712
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5732..5752
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgagtgta ctgccccctg tacgaaaaga
      781 ccgcataatc ggacagcttc ctaagtgctt caacagaggc gctgctttga acacagagaa
      841 tgaatttcac agcaaggtga agacggcctg tcagcagcag aggacaggaa ctgtggggtt
      901 taagatctcc aaggttattg tagttggtga tctggcggtg gggaaaacct gtttaattaa
      961 tagattttgt aaagatgtct ttgacaaaaa ttacaaagct accattggtg tggactttga
     1021 aatggagagg tttgaagttc tcggagtccc cttcagtctg caactatggg acactgcagg
     1081 acaggagagg tttaaatgca ttgcctcaac atactacaga ggagctcagg ctgtaatcat
     1141 tgtgtttgac ctgactgatg tggcttcact ggagcacacc agacaatggc ttgaagacgc
     1201 tatgaaagag aatgatccta ccagtgtatt actgttctta gtgggaacaa agaaagatct
     1261 gagttctcct gcgcagtatt cactaattga gcaggacgca attaaaatag cggatcagat
     1321 gaaagcggag tactgggctt tgtctgcctt gtctggggag aatgtgagag actttttctt
     1381 cagagtcgca tctttaacct ttgaggccaa tgtactggca gaactggaga aaagtggatc
     1441 aaggcgtatt ggggaagttg ttcaaatcag cagcaactct aataacctgt acgcaacgtc
     1501 caagaagaaa cagagcaact gttgtcagta gcaactttat tatacatagt tgataattca
     1561 ctggccgtcg ttttacggta ccatcgatga tgatccagac atgataagat acattgatga
     1621 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     1681 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     1741 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     1801 cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctgcg aagatacggc
     1861 cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa
     1921 tcacatactt cctccatttt cttccactga ttgactgtta taatttccct aatttccagg
     1981 tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg
     2041 aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca
     2101 tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag
     2161 aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa
     2221 aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat
     2281 tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa
     2341 ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat
     2401 ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     2461 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2521 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2581 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2641 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2701 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3061 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3301 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3541 ggcattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4501 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4621 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4681 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4741 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4801 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4861 cacgctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa
     4921 ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact
     4981 acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt
     5041 gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca
     5101 tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa
     5161 aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat
     5221 taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta
     5281 aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct
     5341 tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct
     5401 ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg
     5461 ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt
     5521 agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc
     5581 acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg
     5641 aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc tcgagcagga
     5701 aacagctatg accatgatta cgccaagcta tcaactttgt atagaaaagt tggcacgcgc
     5761 agattccgtg tgggcctggt aatatcttac cattttaagc tattttaaga ttagggaaaa
     5821 aaattaaata aaactacttt aaaattactt tccttaaaaa gcaacatatt tatttagttt
     5881 ttgtttaact ggctcaagat tgtaatattt tacttttaca tgttttttta agtacctaaa
     5941 tgtgttcaaa agtaaagtat gttattgttt ttttttttta atgttagcta agctttaaat
     6001 tgtaatatat tacttttaaa agtgactttt taaaaatgta aataaaaaag taactataaa
     6061 tgtgtaatgc atgttatttc tgtgggtttt taattttcat ctcaactggg cttacaattt
     6121 taacataata aaaatcaaat caaatcaaat caggccctta tttgtcacat acactcttgt
     6181 atactgaaat aaactaaatg ttgagaaaaa tttaaataca ccaaaataac gcataccttt
     6241 tttgtaatta cctaagtaaa acatttagtt ccttttttaa gtgactcaag attgtaatct
     6301 attaattgta aaagataaaa ctagtgatca atctgacgtg cttctatgca gcctgtgaga
     6361 ttaattgtga tgtttaatta ttaaacaatt taaatggatc aaacatttca catgtgctta
     6421 ttgtgtccaa caaaaccagc agaagcactt aaaaatattg catgacaaca tgagttttta
     6481 atgtaaatta tatatacttc ctaaacgaag ttaatcatat ttctaaaata ataatgtcat
     6541 tgaaatattt aaataaatga taagctttag ttgattcaac ataaaattga aagcaataag
     6601 gacttttttt ttttacagcg gtgaagcaaa agcatgtaat aatcatataa tataataaat
     6661 aaataaataa ataatggctc ttggtgcaaa tatgaacact ttaaataaat atagaataaa
     6721 tatgatcaat aagtcataac gatgtgtttt atgttgatta taatttaaac atgtttctaa
     6781 cgcaagtttt aaaaactgtt ttaaagtatt attttaagtt ataagatcaa tttgactcaa
     6841 cattttgtag tgaaaatatg ttgtgtaaaa ttaatataat atacactata aaaatgcagg
     6901 gttccacaca atacattaat gttgtcccaa cacaaatcga ttaagttaac aaatatatgt
     6961 ggattgaaca taaaaaatta agttgtccca atgaaatctc aagaattgtg ttgtttcagc
     7021 tcattttaaa taagtagttt taacaagtaa aaaataacat atttttgagt gtgtatatat
     7081 atatattaca aaatataata atataaaatg taaaagatat aaaccatgct acatatattt
     7141 aagagctatt ttgcacatta cttttttgca agccatttta gtgttttttt tacaaatata
     7201 tattagctaa atatacaata tcttaaatat acaattaatc agacatgcaa ttaatgcatt
     7261 taacctacat aaaaatacat gatcaacata tgttttatgc tgattacaac taaatcaaac
     7321 gtaatgaaat taatttataa aagcgattta aatatgtcaa tattttaagt taaaatcact
     7381 tgaaacctac tataaatgaa cttaaattaa aaaaatctcc tttaattcca atgcaacagt
     7441 gttgtttttt cagctgagga cccccggtgc agatggttgg cccccggccc gctattctaa
     7501 ggaagccttt cttaatttta gggcggcgct gcatgcgctc agatcagatt cacgggtgga
     7561 gagggttatt ttctcttttt tattttttct tgcctgctca tatttgggcc tgtgtgaacc
     7621 ccaggctccg cctcggcccc caggagccgc tcttatttaa aggggatcct cgccttcagt
     7681 cttcgtccca gtggaagcat cagtcttgtg ataacaactt ggtgagtcct acagaccagc
     7741 gctcgactct aggctttaaa tgcattgatt tctctggatt agctaccggg agagttttaa
     7801 ctttgcttat tagtcttgtg ctcgaactgc taggtttaaa gagtgttttc tctaatgcat
     7861 gcatgaagcg acagctgaaa accagaaata gagcgtttgt cctcctgtta aactctagcc
     7921 tttctttggc atttgtcagt ccgagtcttc agctgccggc aggagattgg gtttcagtaa
     7981 cacttgctga gcacaaacta cacgtgcttt cttcagattt aattcattat taatcattct
     8041 agatttaaat gatgttctta ctcattgctg attattttag ccttgcggag cagattaaat
     8101 gagcacgtgt ttcattaaga ttgatttaaa tataattaat atttcaccac tgtagtttta
     8161 aaattgcctt ctaagtcatg gttgattttt aataggtatc ggctctaatt agattactat
     8221 tttggttttt aaatgttatt tctatttcat ttgtaggctc gtttttaatg tgattttatt
     8281 agattctagt tttaaaatca tctcttagcc ttaatgagca gactgaatga acatgtttca
     8341 ttaagattaa cttcattata atgaattatt taatcattct agtgggttat aaatcagctc
     8401 tatttaaatt aattgtcgtg tttatttcta tttaattttt gattattttt tcagggtcgt
     8461 ttttatgtga ttttttcccg tttctaattt taaactaatt gtttgatttt tatatgattc
     8521 gactcttttt aaatgaattg ttttgctttt tttattgagc atatatatat tatacaattt
     8581 caatcaacaa ttgttgtgct ttttaaatgt tgtttctatt tcatttctag ggtcgatttt
     8641 attcgattct agttttaaaa tcgtgatttt taacttctaa ccttactgag cagactgaat
     8701 gaacatgttt catcaagatt aacttcatta tagttaatta ttgaatcgtg ctagtggttt
     8761 ataaatcagc tctatttaaa ttaatcatca tcattatttc tatttaattt ttattatttc
     8821 tttaggatta tttttatgtg atttttttca cgttctagtt ttaaactcaa tgctcattac
     8881 tgctcaccct tgctgagcag tctttcgtta agattaaatt aattaataac cattctagtt
     8941 ttaaaacagt gtcttaaggc atagctgata ttttacaagt ttactattta atatatctcc
     9001 tttctttttt tagggtcgat ttcatctgat ttgattaatt tttattatct tcactaagct
     9061 ttttgttcta ggttttattc tcagtattat tactgttata tttgaactga atttctcctc
     9121 atagtttatt cattttattt atttattttt tgtttattcg ctattttgta ttttagtttt
     9181 taattagaaa ttttattaga atcgttttga actagttttt atgcactcta acattaatat
     9241 taaatcataa ctattatttt tattataatt tatttgtgtt tatctttttg tgtagctttc
     9301 atgtgttttt attttagttt tattatgttt gcattgaatt ttttggacta gtttatataa
     9361 cgatatacag aaaagccatg acaacaaatg tttttataat acctattaag tataagctaa
     9421 tcagatttcc actattctgt tcaaagttaa cattattagc cattctgatg gatcatttga
     9481 catttgtcat atgtctttat gaacactgct ctttcagatg ccacttttaa agctgttttt
     9541 tacagtaaat gaacgtttat tattatgaca ttatttccta aggcctctag agtacagcag
     9601 gatgtggcgt gtgcattacc gtgtaattat gctgactgta aatgtgcatt tatagactgt
     9661 cagcatgtct gactgtgcgc tgttgtgatt ggtcctgcac agaccaag
//
