LOCUS       sequence-22123-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   McMahon LP, Choi KM, Lin TA, Abraham RT, Lawrence JC
  TITLE     The rapamycin-binding domain governs substrate selectivity by the 
            mammalian target of rapamycin.
  JOURNAL   Mol Cell Biol. 2002 Nov . 22(21):7428-38.
  PUBMED    12370290
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2002 Nov . 22(21):7428-38."
COMMENT     Sequence Label: pcDNA3 AU1 mTOR S2035W
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cntggggcnt tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga
       61 aattaatacg actcactata gggagaccca agcttctcga gccaccatgg acacgtacag
      121 gtacatcctt gggacaggcc ctgccacggc caccgccggt gccgccacat ccagcaacgt
      181 gagcgttctg cagcagttcg ccagtgggct gaagagccgg aatgaggaga ccagggccaa
      241 agcagccaag gagctccagc actatgtcac catggaactt cgagagatga gtcaggagga
      301 gtctactcgc ttctatgacc agctgaacca tcacattttt gaactggttt ccagctcaga
      361 cgccaatgag aggaagggtg gcatcttggc cattgccagc ctcattggag tggaaggtgg
      421 gaattccacc aggattggca gatttgccaa ctaccttcgg aacctcctcc cctcaagtga
      481 tccagttgtc atggagatgg catccaaggc cattggccgc cttgcaatgg caggggacac
      541 tttcactgct gagtatgtgg agtttgaagt gaagcgagcc ttggagtggc tgggtgctga
      601 ccgaaatgag ggccggagac atgcagctgt cctcgttctc cgtgagctgg ccatcagcgt
      661 ccccaccttc ttcttccagc aagttcagcc cttctttgac aacatttttg tggctgtgtg
      721 ggaccccaag caggccatcc gtgaaggagc tgtggctgcc ctt
//
