LOCUS       sequence-221230-        9698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab34b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab34b
FEATURES             Location/Qualifiers
     source          1..9698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1522..1542)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1550..1566)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1582..1716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1606..1625)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1660..1679
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2155..2342,2343..2354)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2388..2404)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2388..2404)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2441..2461)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2441..2459)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2480..2496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2493..2515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2504..2520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2528..2534,2535..2552,2553..2558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2573..2594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2711..2728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2882..3470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2962..2981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3641..4432,4433..4501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4264..4283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4502..4606)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4769..4790)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5139..5150,5151..5313)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5686..5702
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5722..5742
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgatgaat tctttacccc ctgtgaaagg
      781 agaccggatc atctgtgaac tccctttgta ttttacaaga gatgctgcag tgcacaccaa
      841 agatggcttc aatattactg ttagagaggc ttgtcaaggg cagaaaacag gattaaatgt
      901 agccaaagtc attgtcgttg gtgatgttgg ggtcgggaag acctgtttaa taaacagatt
      961 ctgcaaagac tcatttgaca gaacgtacaa ggcaactatt ggagtggatt ttgagatgga
     1021 gagatttgaa gttctcgggg tacccttcag tttgcagctg tgggacacag caggtcagga
     1081 aagatttcga tgcattgcct ccacatacta cagaggagcg caagccatca tcgttgtgtt
     1141 tgacctgagc aactatgagt ctctggatca cgcgagagaa tggcttgaag atgccatgag
     1201 ggacaatgac ccgtccagtg tcctgctctt cctcgtggga accaagaagg atcttagtca
     1261 tccagatctg ttggcccaaa tggagcagga ggccattcga ttgtctgagg agatcagagc
     1321 agagtactgg gcagtttctg ctttgtcagg ggcgagtgtg agagatttct tcctgcgggt
     1381 tgcttcgctt acatttgagg ccactgtttt atctgagctg gagaagaaca attctagacg
     1441 agccggtgat atcgtcaaaa tctccaataa ttgtgacaac aattttaaga agaggcaatc
     1501 cagctgctgc cattgactga acaactttat tatacatagt tgataattca ctggccgtcg
     1561 ttttacggta ccatcgatga tgatccagac atgataagat acattgatga gtttggacaa
     1621 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     1681 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     1741 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     1801 tgtggtatgg ctgattatga tcctctagat cagatctgcg aagatacggc cacgggtgct
     1861 cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa tcacatactt
     1921 cctccatttt cttccactga ttgactgtta taatttccct aatttccagg tcaaggtgct
     1981 gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg aacatgtctg
     2041 accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca tttaaattaa
     2101 actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag aatgaagtga
     2161 tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt
     2221 tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag
     2281 taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt
     2341 actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat ccactagttc
     2401 tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg
     2461 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2521 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2821 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2881 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2941 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3001 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3061 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3301 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa ggcattttgg
     3541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4621 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     4681 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4741 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4801 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cacgctggct
     4861 aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa
     4921 tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc
     4981 caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc
     5041 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
     5101 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
     5161 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
     5221 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
     5281 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
     5341 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
     5401 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
     5461 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
     5521 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
     5581 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
     5641 agagactccc tggtgtctga aacacaggcc agatgggccc tcgagcagga aacagctatg
     5701 accatgatta cgccaagcta tcaactttgt atagaaaagt tggcacgcgc agattccgtg
     5761 tgggcctggt aatatcttac cattttaagc tattttaaga ttagggaaaa aaattaaata
     5821 aaactacttt aaaattactt tccttaaaaa gcaacatatt tatttagttt ttgtttaact
     5881 ggctcaagat tgtaatattt tacttttaca tgttttttta agtacctaaa tgtgttcaaa
     5941 agtaaagtat gttattgttt ttttttttta atgttagcta agctttaaat tgtaatatat
     6001 tacttttaaa agtgactttt taaaaatgta aataaaaaag taactataaa tgtgtaatgc
     6061 atgttatttc tgtgggtttt taattttcat ctcaactggg cttacaattt taacataata
     6121 aaaatcaaat caaatcaaat caggccctta tttgtcacat acactcttgt atactgaaat
     6181 aaactaaatg ttgagaaaaa tttaaataca ccaaaataac gcataccttt tttgtaatta
     6241 cctaagtaaa acatttagtt ccttttttaa gtgactcaag attgtaatct attaattgta
     6301 aaagataaaa ctagtgatca atctgacgtg cttctatgca gcctgtgaga ttaattgtga
     6361 tgtttaatta ttaaacaatt taaatggatc aaacatttca catgtgctta ttgtgtccaa
     6421 caaaaccagc agaagcactt aaaaatattg catgacaaca tgagttttta atgtaaatta
     6481 tatatacttc ctaaacgaag ttaatcatat ttctaaaata ataatgtcat tgaaatattt
     6541 aaataaatga taagctttag ttgattcaac ataaaattga aagcaataag gacttttttt
     6601 ttttacagcg gtgaagcaaa agcatgtaat aatcatataa tataataaat aaataaataa
     6661 ataatggctc ttggtgcaaa tatgaacact ttaaataaat atagaataaa tatgatcaat
     6721 aagtcataac gatgtgtttt atgttgatta taatttaaac atgtttctaa cgcaagtttt
     6781 aaaaactgtt ttaaagtatt attttaagtt ataagatcaa tttgactcaa cattttgtag
     6841 tgaaaatatg ttgtgtaaaa ttaatataat atacactata aaaatgcagg gttccacaca
     6901 atacattaat gttgtcccaa cacaaatcga ttaagttaac aaatatatgt ggattgaaca
     6961 taaaaaatta agttgtccca atgaaatctc aagaattgtg ttgtttcagc tcattttaaa
     7021 taagtagttt taacaagtaa aaaataacat atttttgagt gtgtatatat atatattaca
     7081 aaatataata atataaaatg taaaagatat aaaccatgct acatatattt aagagctatt
     7141 ttgcacatta cttttttgca agccatttta gtgttttttt tacaaatata tattagctaa
     7201 atatacaata tcttaaatat acaattaatc agacatgcaa ttaatgcatt taacctacat
     7261 aaaaatacat gatcaacata tgttttatgc tgattacaac taaatcaaac gtaatgaaat
     7321 taatttataa aagcgattta aatatgtcaa tattttaagt taaaatcact tgaaacctac
     7381 tataaatgaa cttaaattaa aaaaatctcc tttaattcca atgcaacagt gttgtttttt
     7441 cagctgagga cccccggtgc agatggttgg cccccggccc gctattctaa ggaagccttt
     7501 cttaatttta gggcggcgct gcatgcgctc agatcagatt cacgggtgga gagggttatt
     7561 ttctcttttt tattttttct tgcctgctca tatttgggcc tgtgtgaacc ccaggctccg
     7621 cctcggcccc caggagccgc tcttatttaa aggggatcct cgccttcagt cttcgtccca
     7681 gtggaagcat cagtcttgtg ataacaactt ggtgagtcct acagaccagc gctcgactct
     7741 aggctttaaa tgcattgatt tctctggatt agctaccggg agagttttaa ctttgcttat
     7801 tagtcttgtg ctcgaactgc taggtttaaa gagtgttttc tctaatgcat gcatgaagcg
     7861 acagctgaaa accagaaata gagcgtttgt cctcctgtta aactctagcc tttctttggc
     7921 atttgtcagt ccgagtcttc agctgccggc aggagattgg gtttcagtaa cacttgctga
     7981 gcacaaacta cacgtgcttt cttcagattt aattcattat taatcattct agatttaaat
     8041 gatgttctta ctcattgctg attattttag ccttgcggag cagattaaat gagcacgtgt
     8101 ttcattaaga ttgatttaaa tataattaat atttcaccac tgtagtttta aaattgcctt
     8161 ctaagtcatg gttgattttt aataggtatc ggctctaatt agattactat tttggttttt
     8221 aaatgttatt tctatttcat ttgtaggctc gtttttaatg tgattttatt agattctagt
     8281 tttaaaatca tctcttagcc ttaatgagca gactgaatga acatgtttca ttaagattaa
     8341 cttcattata atgaattatt taatcattct agtgggttat aaatcagctc tatttaaatt
     8401 aattgtcgtg tttatttcta tttaattttt gattattttt tcagggtcgt ttttatgtga
     8461 ttttttcccg tttctaattt taaactaatt gtttgatttt tatatgattc gactcttttt
     8521 aaatgaattg ttttgctttt tttattgagc atatatatat tatacaattt caatcaacaa
     8581 ttgttgtgct ttttaaatgt tgtttctatt tcatttctag ggtcgatttt attcgattct
     8641 agttttaaaa tcgtgatttt taacttctaa ccttactgag cagactgaat gaacatgttt
     8701 catcaagatt aacttcatta tagttaatta ttgaatcgtg ctagtggttt ataaatcagc
     8761 tctatttaaa ttaatcatca tcattatttc tatttaattt ttattatttc tttaggatta
     8821 tttttatgtg atttttttca cgttctagtt ttaaactcaa tgctcattac tgctcaccct
     8881 tgctgagcag tctttcgtta agattaaatt aattaataac cattctagtt ttaaaacagt
     8941 gtcttaaggc atagctgata ttttacaagt ttactattta atatatctcc tttctttttt
     9001 tagggtcgat ttcatctgat ttgattaatt tttattatct tcactaagct ttttgttcta
     9061 ggttttattc tcagtattat tactgttata tttgaactga atttctcctc atagtttatt
     9121 cattttattt atttattttt tgtttattcg ctattttgta ttttagtttt taattagaaa
     9181 ttttattaga atcgttttga actagttttt atgcactcta acattaatat taaatcataa
     9241 ctattatttt tattataatt tatttgtgtt tatctttttg tgtagctttc atgtgttttt
     9301 attttagttt tattatgttt gcattgaatt ttttggacta gtttatataa cgatatacag
     9361 aaaagccatg acaacaaatg tttttataat acctattaag tataagctaa tcagatttcc
     9421 actattctgt tcaaagttaa cattattagc cattctgatg gatcatttga catttgtcat
     9481 atgtctttat gaacactgct ctttcagatg ccacttttaa agctgttttt tacagtaaat
     9541 gaacgtttat tattatgaca ttatttccta aggcctctag agtacagcag gatgtggcgt
     9601 gtgcattacc gtgtaattat gctgactgta aatgtgcatt tatagactgt cagcatgtct
     9661 gactgtgcgc tgttgtgatt ggtcctgcac agaccaag
//
