LOCUS       sequence-221232-        9544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab35b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab35b
FEATURES             Location/Qualifiers
     source          1..9544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1368..1388)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1396..1412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1428..1562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1452..1471)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1506..1525
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2001..2188,2189..2200)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2234..2250)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2234..2250)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2287..2307)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2287..2305)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2326..2342)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2339..2361)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2350..2366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2374..2380,2381..2398,2399..2404))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2419..2440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2557..2574)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2728..3316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2808..2827)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3487..4278,4279..4347))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4110..4129
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4348..4452)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4615..4636)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(4985..4996,4997..5159)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5532..5548
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5568..5588
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcccgc gactacgatc acctcttcaa
      781 gctgctgatc atcggtgaca gcggtgttgg aaagagcagt cttctcctgc gtttcgccga
      841 caacacattt tcaggcagtt acatcaccac cataggagtc gacttcaaga tccgcactgt
      901 ggagcttaat ggagagaagg tgaaactgca gatatgggac acagccggac aagagcgatt
      961 ccgaacgatt acatctacat attacagagg aacccatggt gtgatagtag tgtatgacgt
     1021 taccagcgcc gagtcctttg tcaatgttaa acgatggcta catgaaataa accagaactg
     1081 tgatgacgtc tgtcgaatat tagtgggcaa taaaaatgat gatccaaact ctaaggtggt
     1141 ggagacgaat gatgcacaga agtttgcaga acagatgggc atcagtctgt tcgagacaag
     1201 tgcgaaagaa aatgtcaatg tggaggagat gtttaactgc atcacagagt tggttctccg
     1261 ggcaaagaaa gagtctgtgg ccaaacagca gcagcagcag cagaacgatg tgattaaact
     1321 caagcagaac agcaaacgga agaagaagtg ctgctagtgg acgaacacaa ctttattata
     1381 catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat ccagacatga
     1441 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     1501 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     1561 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     1621 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga
     1681 tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct
     1741 cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat
     1801 ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac
     1861 ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata
     1921 ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa
     1981 tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata
     2041 aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa
     2101 agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta
     2161 cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc
     2221 agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
     2281 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     2341 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2401 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2461 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2521 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2581 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2641 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2701 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2761 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2821 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2881 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2941 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3001 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3061 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3121 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3181 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3241 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3301 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3361 cgaaaactca cgttaaggca ttttggtcat gagattatca aaaaggatct tcacctagat
     3421 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3481 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3541 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3601 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3661 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3721 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3781 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3841 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3901 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3961 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4021 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4081 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4141 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4201 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4261 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4321 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4381 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4441 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     4501 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     4561 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     4621 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     4681 tatcagggcg atggcccacg ctggctaaga actcatcagc ctccccggtc catctaccca
     4741 cgtaccaatg caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt
     4801 tataggaatg gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg
     4861 ttggacccag gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata
     4921 tctgttcaga cacccatatc cactctgttc cacacaggtc agaggtttgt ccaggagttc
     4981 ttgacagagg tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca agtacttagg
     5041 gaaaatttta ctcaattaaa agtaaaagta tctggctaga atcttacttg agtaaaagta
     5101 aaaaagtact ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa
     5161 gaaaactaga gattcttgtt taagctttta atctcaaaaa acattaaatg aaatgcatac
     5221 aaggttttat cctgctttag aactgtttgt atttaattat caaactataa gacagacaat
     5281 ctaatgccag tacacgctac tcaaagttgt aaaacctcag atttaacttc agtagaagct
     5341 gattctcaaa attgttagtg tcaagcctag ctcttttggg gctgaaaagc aatcctgcag
     5401 tgctgaaaag cctctcacag gcagccgatg cgggaagagg tgtattagtc ttgatagaga
     5461 ggctgcaaat agcaggaaac gtgagcagag actccctggt gtctgaaaca caggccagat
     5521 gggccctcga gcaggaaaca gctatgacca tgattacgcc aagctatcaa ctttgtatag
     5581 aaaagttggc acgcgcagat tccgtgtggg cctggtaata tcttaccatt ttaagctatt
     5641 ttaagattag ggaaaaaaat taaataaaac tactttaaaa ttactttcct taaaaagcaa
     5701 catatttatt tagtttttgt ttaactggct caagattgta atattttact tttacatgtt
     5761 tttttaagta cctaaatgtg ttcaaaagta aagtatgtta ttgttttttt tttttaatgt
     5821 tagctaagct ttaaattgta atatattact tttaaaagtg actttttaaa aatgtaaata
     5881 aaaaagtaac tataaatgtg taatgcatgt tatttctgtg ggtttttaat tttcatctca
     5941 actgggctta caattttaac ataataaaaa tcaaatcaaa tcaaatcagg cccttatttg
     6001 tcacatacac tcttgtatac tgaaataaac taaatgttga gaaaaattta aatacaccaa
     6061 aataacgcat accttttttg taattaccta agtaaaacat ttagttcctt ttttaagtga
     6121 ctcaagattg taatctatta attgtaaaag ataaaactag tgatcaatct gacgtgcttc
     6181 tatgcagcct gtgagattaa ttgtgatgtt taattattaa acaatttaaa tggatcaaac
     6241 atttcacatg tgcttattgt gtccaacaaa accagcagaa gcacttaaaa atattgcatg
     6301 acaacatgag tttttaatgt aaattatata tacttcctaa acgaagttaa tcatatttct
     6361 aaaataataa tgtcattgaa atatttaaat aaatgataag ctttagttga ttcaacataa
     6421 aattgaaagc aataaggact tttttttttt acagcggtga agcaaaagca tgtaataatc
     6481 atataatata ataaataaat aaataaataa tggctcttgg tgcaaatatg aacactttaa
     6541 ataaatatag aataaatatg atcaataagt cataacgatg tgttttatgt tgattataat
     6601 ttaaacatgt ttctaacgca agttttaaaa actgttttaa agtattattt taagttataa
     6661 gatcaatttg actcaacatt ttgtagtgaa aatatgttgt gtaaaattaa tataatatac
     6721 actataaaaa tgcagggttc cacacaatac attaatgttg tcccaacaca aatcgattaa
     6781 gttaacaaat atatgtggat tgaacataaa aaattaagtt gtcccaatga aatctcaaga
     6841 attgtgttgt ttcagctcat tttaaataag tagttttaac aagtaaaaaa taacatattt
     6901 ttgagtgtgt atatatatat attacaaaat ataataatat aaaatgtaaa agatataaac
     6961 catgctacat atatttaaga gctattttgc acattacttt tttgcaagcc attttagtgt
     7021 tttttttaca aatatatatt agctaaatat acaatatctt aaatatacaa ttaatcagac
     7081 atgcaattaa tgcatttaac ctacataaaa atacatgatc aacatatgtt ttatgctgat
     7141 tacaactaaa tcaaacgtaa tgaaattaat ttataaaagc gatttaaata tgtcaatatt
     7201 ttaagttaaa atcacttgaa acctactata aatgaactta aattaaaaaa atctccttta
     7261 attccaatgc aacagtgttg ttttttcagc tgaggacccc cggtgcagat ggttggcccc
     7321 cggcccgcta ttctaaggaa gcctttctta attttagggc ggcgctgcat gcgctcagat
     7381 cagattcacg ggtggagagg gttattttct cttttttatt ttttcttgcc tgctcatatt
     7441 tgggcctgtg tgaaccccag gctccgcctc ggcccccagg agccgctctt atttaaaggg
     7501 gatcctcgcc ttcagtcttc gtcccagtgg aagcatcagt cttgtgataa caacttggtg
     7561 agtcctacag accagcgctc gactctaggc tttaaatgca ttgatttctc tggattagct
     7621 accgggagag ttttaacttt gcttattagt cttgtgctcg aactgctagg tttaaagagt
     7681 gttttctcta atgcatgcat gaagcgacag ctgaaaacca gaaatagagc gtttgtcctc
     7741 ctgttaaact ctagcctttc tttggcattt gtcagtccga gtcttcagct gccggcagga
     7801 gattgggttt cagtaacact tgctgagcac aaactacacg tgctttcttc agatttaatt
     7861 cattattaat cattctagat ttaaatgatg ttcttactca ttgctgatta ttttagcctt
     7921 gcggagcaga ttaaatgagc acgtgtttca ttaagattga tttaaatata attaatattt
     7981 caccactgta gttttaaaat tgccttctaa gtcatggttg atttttaata ggtatcggct
     8041 ctaattagat tactattttg gtttttaaat gttatttcta tttcatttgt aggctcgttt
     8101 ttaatgtgat tttattagat tctagtttta aaatcatctc ttagccttaa tgagcagact
     8161 gaatgaacat gtttcattaa gattaacttc attataatga attatttaat cattctagtg
     8221 ggttataaat cagctctatt taaattaatt gtcgtgttta tttctattta atttttgatt
     8281 attttttcag ggtcgttttt atgtgatttt ttcccgtttc taattttaaa ctaattgttt
     8341 gatttttata tgattcgact ctttttaaat gaattgtttt gcttttttta ttgagcatat
     8401 atatattata caatttcaat caacaattgt tgtgcttttt aaatgttgtt tctatttcat
     8461 ttctagggtc gattttattc gattctagtt ttaaaatcgt gatttttaac ttctaacctt
     8521 actgagcaga ctgaatgaac atgtttcatc aagattaact tcattatagt taattattga
     8581 atcgtgctag tggtttataa atcagctcta tttaaattaa tcatcatcat tatttctatt
     8641 taatttttat tatttcttta ggattatttt tatgtgattt ttttcacgtt ctagttttaa
     8701 actcaatgct cattactgct cacccttgct gagcagtctt tcgttaagat taaattaatt
     8761 aataaccatt ctagttttaa aacagtgtct taaggcatag ctgatatttt acaagtttac
     8821 tatttaatat atctcctttc tttttttagg gtcgatttca tctgatttga ttaattttta
     8881 ttatcttcac taagcttttt gttctaggtt ttattctcag tattattact gttatatttg
     8941 aactgaattt ctcctcatag tttattcatt ttatttattt attttttgtt tattcgctat
     9001 tttgtatttt agtttttaat tagaaatttt attagaatcg ttttgaacta gtttttatgc
     9061 actctaacat taatattaaa tcataactat tatttttatt ataatttatt tgtgtttatc
     9121 tttttgtgta gctttcatgt gtttttattt tagttttatt atgtttgcat tgaatttttt
     9181 ggactagttt atataacgat atacagaaaa gccatgacaa caaatgtttt tataatacct
     9241 attaagtata agctaatcag atttccacta ttctgttcaa agttaacatt attagccatt
     9301 ctgatggatc atttgacatt tgtcatatgt ctttatgaac actgctcttt cagatgccac
     9361 ttttaaagct gttttttaca gtaaatgaac gtttattatt atgacattat ttcctaaggc
     9421 ctctagagta cagcaggatg tggcgtgtgc attaccgtgt aattatgctg actgtaaatg
     9481 tgcatttata gactgtcagc atgtctgact gtgcgctgtt gtgattggtc ctgcacagac
     9541 caag
//
