LOCUS       sequence-221235-        6566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: LOC5574680 Atrial natriuretic peptide receptor
FEATURES             Location/Qualifiers
     source          1..6566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    4454..4588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4491..4510)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4545..4564
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4627..4644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4798..5386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4878..4897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5557..6348,6349..6417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6180..6199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6418..6522)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(6562..6566,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgcatgggaa acccttccat tttccggtga ttctactgct
      841 cctgtgccag tactgtgtct tatcattttg catacgaggt aaaccaccat cctcctccgg
      901 gttggcgccg gaagactccg atgtagccat gctgttgaat aatcttagcg attcggtgag
      961 agaacggcca gatgaggtgt cgtacttcga agaggacgag ctgggattgt cgattgtgat
     1021 gggggcagct ggaagcaata ttggtcatcg ggcagccaaa cggtcggccg attggattaa
     1081 tcgcatcgag gtacaggacg acgatatcag tgctaattac atcgtataca acgttggagt
     1141 gctaatggca tctcatttgg attcgccatt cgatttggaa cgatgcggac cagcagtaga
     1201 cttagcgtta gattttataa acgatttttt gggaaagaca catagcatca aactgaagaa
     1261 agttcaagca agctacgcct cctgctcggg agccaaatcg cccggccttg cggcggatat
     1321 gcacttcaag aacaacgtgg tggccttcat cggacctgca tgtgcctttg cgttggaacc
     1381 ggtggcccga ctggccgact actggaatac acccatcatc actggaatgg gcgaccagcc
     1441 cccatcggaa ggtgagctct cggttacatc cggaatcctg ggacgtttga gtaataaatg
     1501 gaaaaacgaa agcacgggaa tattcaagga caaatccaaa tatcagacgt tgactcgcat
     1561 gtcgtactgc cagtgtcgcc tgaagctggt gttctcgagc atttttcaca cgttcgactg
     1621 gaggcacatt gccctgatat tggatcggtc ggatttgttt tcactgacgg tgggaaagaa
     1681 tctcgagtac gggctcaagg aggaagacct tctgacctgc gtgcgggaac tggatggtaa
     1741 cgacgaagag gacctggata actacttgcg ggatgtgagc atgtatgctc gagtgattat
     1801 cttgtccgtg aggggcagtt tggtgcgaaa gttcatgctc tccgcccacc ggctcggtat
     1861 gacgaggggt gagttcacgt ttttagatgt ggaaatcttc caaagctcct actggggcga
     1921 ccactactgg gaattgggcg atgaagacga ccaaactgct cggaaggcgt atgaagccct
     1981 gctgagagtc tctttgcttc agcccacgag cccctcgtac cagattttcg ccgaccaggt
     2041 gagaattcgt gccaaacggg actacaatta cacctttgtg gaggacgagg aggtgaactt
     2101 tttcatcgga gcctttttcg atggtgttta cctgctgggt atggcgctga acgagacact
     2161 gactgagggc ggcaatattc gagacggaac ggccatcact cggaagatgt ggaacagaag
     2221 cttcgacggc attactggaa acgtgcgaat cgacgacaat ggagatcggg acgcagatta
     2281 ctccattctc gacctggacc ccattaccgg acgcttcgag gtggtagccc attattacgg
     2341 acgaacaaga gcttactctc cggtgaaggg caaacgaatc cactggccag gtggccggga
     2401 aggtcctcca cccgatgttc ccaagtgtgg atttttggga acatcaccgt cgtgccaagg
     2461 aagcgaaata attctcatct acgcgttgat cgggtttggg ataatatctg cgttcggcgc
     2521 cgccgtcacg tacattcttt gcaagcaaat gaagttaaat aacgaactga acaacatgtc
     2581 ctggcgggtg cgcccggatg aggttctact ggaagtagga cgacttttcg gcagtaaaat
     2641 gggactgcag aagttgaact atgagaattt ttccctccag caattcggaa gctccggtcg
     2701 tgcgtccatt tccagtggcc actcccaatt gccagcccag ttgttcacca ctatcggcat
     2761 ctacaagggt gagcgagttg ccatcaaaaa ggtagccaaa aagaaggttt acataacctc
     2821 aacgctgctc tgggaaatca aacaagcccg agacgtaagc cacgagaata cagttcgctt
     2881 tgtgggcgca tgcatcgatc taccgaggcc gaccattctg atattgaccg aatactgtcc
     2941 gaaaggtagt ctcaaggatg ttctggaaaa cgaagccatt cagctggatt ggaatttccg
     3001 aatgtcgttg attcatgatg tcgtcaaagg aatggcgtat cttcacaaca gtgacgttgg
     3061 agttcatgga aagctacggt cctgtaactg tctgatcgat ggccgatttg ttctgaagat
     3121 ctccgatttt ggacttcgta ctctgacgac tccgtcggag tttgtgcggg atcaaaacta
     3181 ctacaacaaa ttgagttttc cagagttact gtgggtagca cccgagctgc ttccgacaac
     3241 ggtgatccca ggaacgccgg caactcagaa gggagatgtc tactcgtttg cgatcattct
     3301 ggaggagatc gttgttcgag gtggaccgta cgagacggct cggcagttta tggacccaca
     3361 gcagattgcg gaaagagttg cagctcatga aagccctcct tttagacctt ttgttggcca
     3421 gagagactgt cccccggatt tgctggatct aatggagaaa tgttggtcgg atagtccaga
     3481 cgatcgtcca agtttcattc aaataaagag cacggttaaa ctgattatga aaggtttttg
     3541 cgagaatctt atggacgact tgctgcgtcg tatggagcag tacgccaaca atttggaaag
     3601 cttggtcgaa gagaaaaccg aacagctcag tatggagaaa cggcgtaccg aagagcttct
     3661 gtatcaggtt ctaccgaggc cggtggcaca gcaacttctt gccggggaaa tggtccaacc
     3721 ggagcagttc gaatgtgtca caatctattt cagtgatatt gtaggcttta cggcactttg
     3781 cgctcaaagt cgaccaatgg aggtcgtcga cttcctgaac gatttgtaca gcactttcga
     3841 tagaataatt gaattttacg acgtttacaa ggtggaaacc atcggagatg cctacatggt
     3901 ggtatcggga cttccggagc gaaatggaca cgatcatgcg cgggaaatcg gtttaatgtc
     3961 gttggcgatt ttggatgcgg taaagtcatt cacaatcaag cacaaacctg aacagcagct
     4021 gaagattcgt attgggatcc attcgggacc ggtttgcgct ggagttgttg ggcagaaaat
     4081 gcctcactat tgtttgttcg gtgatacggt taatacggca tcgaggatgg aatctactgg
     4141 acatccattg aaaattcatg tgtctgaggc ggctaaacaa atcctagaca aatttggaac
     4201 ttttcgattg gaattgcgag gagaagttga acttaaagga aaaggaatct gcacaacgtt
     4261 ttggttattg gaatgttctg aaccagatcc aaggccccca acaccgatga aaaactacat
     4321 tgataatgat gttccatttc cgattctctt cccggcaatc ggaaaataag cggccgctag
     4381 actagtctag agaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     4441 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4501 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4561 catcaatgta tcttatcatg tctggatccc cgggtaccga gctcgaatta attcctcttc
     4621 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4681 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4741 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5221 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5281 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5521 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5581 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5641 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5701 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5761 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5821 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5881 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5941 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6001 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6061 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6121 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6181 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6241 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6301 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6361 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6421 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6481 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6541 gccacctgca agcttggctg tggaat
//
