LOCUS       sequence-221238-        9569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab39a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9569)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab39a
FEATURES             Location/Qualifiers
     source          1..9569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1393..1413)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1421..1437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1453..1587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1477..1496)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1531..1550
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2026..2213,2214..2225)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2259..2275)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2259..2275)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2312..2332)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2312..2330)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2351..2367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2364..2386)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2375..2391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2399..2405,2406..2423,2424..2429))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2444..2465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2582..2599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2753..3341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2833..2852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3512..4303,4304..4372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4135..4154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4373..4477)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4640..4661)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5010..5021,5022..5184)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5557..5573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5593..5613
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggaggtg ccgtggaact tcaggttcgg
      781 gatcgtgatt ttaggagact cttttgtggg gaaatcctct cttcttcatc gcttcactga
      841 aggcacgttc gaggaggccc gtcagtctcc tctgggaatc gattttaagg tgcataacat
      901 gaagattgag cctaatgtgc tgattaaact gcttctgtgg gacacggcag gacaggagag
      961 attcaggtca atctgtaaat cctacatgag gaactctgtt ggctgtatcc tgatgtttga
     1021 cgtgtctcag cggaagtcct ttgagcacgt ttccctctgg catcaggaag tgctggatta
     1081 cgtgaagcca aacccgatga tattcctgct ggtggctcat aaaagtgacc tggccgtgtg
     1141 cagagaggtc agaaagagtg aaggagaagc tctggctgag aaactcaaca tgctggccta
     1201 tctggaggta tccacaaaag actacattaa cgtcagcgag gcgttcgaga ctctgaccac
     1261 gggaatatat cagcagttcc agaaaggcga aatctccgtt cgtgatgact ggcacggttt
     1321 aacggttggg acttccgtta aaaacgagaa aaccatgaca aaaaagtctg cctgctgcaa
     1381 ctgcggatga gccaacttta ttatacatag ttgataattc actggccgtc gttttacggt
     1441 accatcgatg atgatccaga catgataaga tacattgatg agtttggaca aaccacaact
     1501 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     1561 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     1621 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     1681 gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc tcttgatcct
     1741 gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt
     1801 tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt
     1861 ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc
     1921 atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat
     1981 cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg atctccaaaa
     2041 aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa
     2101 aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa
     2161 aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag tactttacac
     2221 ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt ctagagcggc
     2281 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     2341 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2401 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2461 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2521 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2581 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2641 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2701 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2761 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2821 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2881 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2941 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3001 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3061 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3121 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3181 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3241 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3301 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3361 ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg gtcatgagat
     3421 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3481 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3541 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3601 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3661 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3721 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3781 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3841 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3901 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3961 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4021 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4081 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4141 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4201 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4261 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4321 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4381 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4441 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4501 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4561 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4621 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4681 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccacgctggc taagaactca
     4741 tcagcctccc cggtccatct acccacgtac caatgcacca attggccaca atgacggcta
     4801 ctacatggtg ccattccttc ctctttatag gaatggagac tacctcctgt ccaacaatgc
     4861 tcttggatac gagtacgcct acctgttgga cccaggtcat tgcacaacac cagaaatgcc
     4921 ctctgatctg caaaagacgt gaatatctgt tcagacaccc atatccactc tgttccacac
     4981 aggtcagagg tttgtccagg agttcttgac agaggtgtaa aaagtactca aaaattttac
     5041 tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg
     5101 ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac ttgagtatta
     5161 aggaagtaaa agtaaaagca agaaagaaaa ctagagattc ttgtttaagc ttttaatctc
     5221 aaaaaacatt aaatgaaatg catacaaggt tttatcctgc tttagaactg tttgtattta
     5281 attatcaaac tataagacag acaatctaat gccagtacac gctactcaaa gttgtaaaac
     5341 ctcagattta acttcagtag aagctgattc tcaaaattgt tagtgtcaag cctagctctt
     5401 ttggggctga aaagcaatcc tgcagtgctg aaaagcctct cacaggcagc cgatgcggga
     5461 agaggtgtat tagtcttgat agagaggctg caaatagcag gaaacgtgag cagagactcc
     5521 ctggtgtctg aaacacaggc cagatgggcc ctcgagcagg aaacagctat gaccatgatt
     5581 acgccaagct atcaactttg tatagaaaag ttggcacgcg cagattccgt gtgggcctgg
     5641 taatatctta ccattttaag ctattttaag attagggaaa aaaattaaat aaaactactt
     5701 taaaattact ttccttaaaa agcaacatat ttatttagtt tttgtttaac tggctcaaga
     5761 ttgtaatatt ttacttttac atgttttttt aagtacctaa atgtgttcaa aagtaaagta
     5821 tgttattgtt tttttttttt aatgttagct aagctttaaa ttgtaatata ttacttttaa
     5881 aagtgacttt ttaaaaatgt aaataaaaaa gtaactataa atgtgtaatg catgttattt
     5941 ctgtgggttt ttaattttca tctcaactgg gcttacaatt ttaacataat aaaaatcaaa
     6001 tcaaatcaaa tcaggccctt atttgtcaca tacactcttg tatactgaaa taaactaaat
     6061 gttgagaaaa atttaaatac accaaaataa cgcatacctt ttttgtaatt acctaagtaa
     6121 aacatttagt tcctttttta agtgactcaa gattgtaatc tattaattgt aaaagataaa
     6181 actagtgatc aatctgacgt gcttctatgc agcctgtgag attaattgtg atgtttaatt
     6241 attaaacaat ttaaatggat caaacatttc acatgtgctt attgtgtcca acaaaaccag
     6301 cagaagcact taaaaatatt gcatgacaac atgagttttt aatgtaaatt atatatactt
     6361 cctaaacgaa gttaatcata tttctaaaat aataatgtca ttgaaatatt taaataaatg
     6421 ataagcttta gttgattcaa cataaaattg aaagcaataa ggactttttt tttttacagc
     6481 ggtgaagcaa aagcatgtaa taatcatata atataataaa taaataaata aataatggct
     6541 cttggtgcaa atatgaacac tttaaataaa tatagaataa atatgatcaa taagtcataa
     6601 cgatgtgttt tatgttgatt ataatttaaa catgtttcta acgcaagttt taaaaactgt
     6661 tttaaagtat tattttaagt tataagatca atttgactca acattttgta gtgaaaatat
     6721 gttgtgtaaa attaatataa tatacactat aaaaatgcag ggttccacac aatacattaa
     6781 tgttgtccca acacaaatcg attaagttaa caaatatatg tggattgaac ataaaaaatt
     6841 aagttgtccc aatgaaatct caagaattgt gttgtttcag ctcattttaa ataagtagtt
     6901 ttaacaagta aaaaataaca tatttttgag tgtgtatata tatatattac aaaatataat
     6961 aatataaaat gtaaaagata taaaccatgc tacatatatt taagagctat tttgcacatt
     7021 acttttttgc aagccatttt agtgtttttt ttacaaatat atattagcta aatatacaat
     7081 atcttaaata tacaattaat cagacatgca attaatgcat ttaacctaca taaaaataca
     7141 tgatcaacat atgttttatg ctgattacaa ctaaatcaaa cgtaatgaaa ttaatttata
     7201 aaagcgattt aaatatgtca atattttaag ttaaaatcac ttgaaaccta ctataaatga
     7261 acttaaatta aaaaaatctc ctttaattcc aatgcaacag tgttgttttt tcagctgagg
     7321 acccccggtg cagatggttg gcccccggcc cgctattcta aggaagcctt tcttaatttt
     7381 agggcggcgc tgcatgcgct cagatcagat tcacgggtgg agagggttat tttctctttt
     7441 ttattttttc ttgcctgctc atatttgggc ctgtgtgaac cccaggctcc gcctcggccc
     7501 ccaggagccg ctcttattta aaggggatcc tcgccttcag tcttcgtccc agtggaagca
     7561 tcagtcttgt gataacaact tggtgagtcc tacagaccag cgctcgactc taggctttaa
     7621 atgcattgat ttctctggat tagctaccgg gagagtttta actttgctta ttagtcttgt
     7681 gctcgaactg ctaggtttaa agagtgtttt ctctaatgca tgcatgaagc gacagctgaa
     7741 aaccagaaat agagcgtttg tcctcctgtt aaactctagc ctttctttgg catttgtcag
     7801 tccgagtctt cagctgccgg caggagattg ggtttcagta acacttgctg agcacaaact
     7861 acacgtgctt tcttcagatt taattcatta ttaatcattc tagatttaaa tgatgttctt
     7921 actcattgct gattatttta gccttgcgga gcagattaaa tgagcacgtg tttcattaag
     7981 attgatttaa atataattaa tatttcacca ctgtagtttt aaaattgcct tctaagtcat
     8041 ggttgatttt taataggtat cggctctaat tagattacta ttttggtttt taaatgttat
     8101 ttctatttca tttgtaggct cgtttttaat gtgattttat tagattctag ttttaaaatc
     8161 atctcttagc cttaatgagc agactgaatg aacatgtttc attaagatta acttcattat
     8221 aatgaattat ttaatcattc tagtgggtta taaatcagct ctatttaaat taattgtcgt
     8281 gtttatttct atttaatttt tgattatttt ttcagggtcg tttttatgtg attttttccc
     8341 gtttctaatt ttaaactaat tgtttgattt ttatatgatt cgactctttt taaatgaatt
     8401 gttttgcttt ttttattgag catatatata ttatacaatt tcaatcaaca attgttgtgc
     8461 tttttaaatg ttgtttctat ttcatttcta gggtcgattt tattcgattc tagttttaaa
     8521 atcgtgattt ttaacttcta accttactga gcagactgaa tgaacatgtt tcatcaagat
     8581 taacttcatt atagttaatt attgaatcgt gctagtggtt tataaatcag ctctatttaa
     8641 attaatcatc atcattattt ctatttaatt tttattattt ctttaggatt atttttatgt
     8701 gatttttttc acgttctagt tttaaactca atgctcatta ctgctcaccc ttgctgagca
     8761 gtctttcgtt aagattaaat taattaataa ccattctagt tttaaaacag tgtcttaagg
     8821 catagctgat attttacaag tttactattt aatatatctc ctttcttttt ttagggtcga
     8881 tttcatctga tttgattaat ttttattatc ttcactaagc tttttgttct aggttttatt
     8941 ctcagtatta ttactgttat atttgaactg aatttctcct catagtttat tcattttatt
     9001 tatttatttt ttgtttattc gctattttgt attttagttt ttaattagaa attttattag
     9061 aatcgttttg aactagtttt tatgcactct aacattaata ttaaatcata actattattt
     9121 ttattataat ttatttgtgt ttatcttttt gtgtagcttt catgtgtttt tattttagtt
     9181 ttattatgtt tgcattgaat tttttggact agtttatata acgatataca gaaaagccat
     9241 gacaacaaat gtttttataa tacctattaa gtataagcta atcagatttc cactattctg
     9301 ttcaaagtta acattattag ccattctgat ggatcatttg acatttgtca tatgtcttta
     9361 tgaacactgc tctttcagat gccactttta aagctgtttt ttacagtaaa tgaacgttta
     9421 ttattatgac attatttcct aaggcctcta gagtacagca ggatgtggcg tgtgcattac
     9481 cgtgtaatta tgctgactgt aaatgtgcat ttatagactg tcagcatgtc tgactgtgcg
     9541 ctgttgtgat tggtcctgca cagaccaag
//
