LOCUS       sequence-221239-        9591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab39ba.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab39ba
FEATURES             Location/Qualifiers
     source          1..9591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1415..1435)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1443..1459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1475..1609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1499..1518)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1553..1572
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2048..2235,2236..2247)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2281..2297)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2281..2297)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2334..2354)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2334..2352)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2373..2389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2386..2408)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2397..2413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2421..2427,2428..2445,2446..2451))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2466..2487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2604..2621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2775..3363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2855..2874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3534..4325,4326..4394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4157..4176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4395..4499)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4662..4683)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5032..5043,5044..5206)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5579..5595
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5615..5635
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggaagct atatggctgt accagttcag
      781 actgattgtc attggcgact cgacagtggg gaagtcttgt ttgatcagac gcttcacaga
      841 aggacgtttt gcacaggtgt ctgacccgac tgttggcgtg gatttcttct cccggctggt
      901 ggagattgaa cctggcaagc gcatcaagct tcagatctgg gacacagcag gccaggagag
      961 gttcagatct atcacaaggg cttattatcg caattcagtg ggtggccttt tgcttttcga
     1021 catcaccaac cggcgctcct tccagaatgt gcacgagtgg ctggaggagg cgcgcagtca
     1081 cgtacagccg cacagcattg tcttcatcct ggtgggacac aagtgcgacc tggagcagca
     1141 gaggcaggtc agccagcagg aggccgaaaa gctggcagcc gcttacggca tgcgctacgt
     1201 ggagacctct gcccgcgacg ccataaacgt ggagaaggcc ttcaccgagc tcacacgcga
     1261 catcttcgag ctggtcaaat gtggggatat taccattcag gagggctggg agggggtcaa
     1321 gagcggcttc gtgcccaatg tggtgcattc gtccgaggag gtgaccaaga gtgaccgccg
     1381 ctgcctctgt tgatcaccag actctcgttt cacccaactt tattatacat agttgataat
     1441 tcactggccg tcgttttacg gtaccatcga tgatgatcca gacatgataa gatacattga
     1501 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     1561 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     1621 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     1681 aaacctctac aaatgtggta tggctgatta tgatcctcta gatcagatct gcgaagatac
     1741 ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat
     1801 gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc
     1861 aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca
     1921 atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata
     1981 acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa
     2041 tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag
     2101 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
     2161 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
     2221 aaattactca agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca
     2281 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     2341 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2401 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2461 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2521 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2581 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2641 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2701 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2761 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2821 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2881 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2941 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3001 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3061 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3121 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3181 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3241 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3301 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3361 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3421 taaggcattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3541 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3601 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3661 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3721 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3781 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3841 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3901 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3961 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4021 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4081 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4141 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4201 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4261 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4321 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4381 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4441 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4501 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     4561 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     4621 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     4681 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4741 gcccacgctg gctaagaact catcagcctc cccggtccat ctacccacgt accaatgcac
     4801 caattggcca caatgacggc tactacatgg tgccattcct tcctctttat aggaatggag
     4861 actacctcct gtccaacaat gctcttggat acgagtacgc ctacctgttg gacccaggtc
     4921 attgcacaac accagaaatg ccctctgatc tgcaaaagac gtgaatatct gttcagacac
     4981 ccatatccac tctgttccac acaggtcaga ggtttgtcca ggagttcttg acagaggtgt
     5041 aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa aattttactc
     5101 aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa aagtactcca
     5161 ttaaaattgt acttgagtat taaggaagta aaagtaaaag caagaaagaa aactagagat
     5221 tcttgtttaa gcttttaatc tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct
     5281 gctttagaac tgtttgtatt taattatcaa actataagac agacaatcta atgccagtac
     5341 acgctactca aagttgtaaa acctcagatt taacttcagt agaagctgat tctcaaaatt
     5401 gttagtgtca agcctagctc ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct
     5461 ctcacaggca gccgatgcgg gaagaggtgt attagtcttg atagagaggc tgcaaatagc
     5521 aggaaacgtg agcagagact ccctggtgtc tgaaacacag gccagatggg ccctcgagca
     5581 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttggcacg
     5641 cgcagattcc gtgtgggcct ggtaatatct taccatttta agctatttta agattaggga
     5701 aaaaaattaa ataaaactac tttaaaatta ctttccttaa aaagcaacat atttatttag
     5761 tttttgttta actgactcaa gattgtaata ttttactttt acatgttttt ttaagtacct
     5821 aaatgtgttc aaaagtaaag tatgttattg tttttttttt ttaatgttag ctaagcttta
     5881 aattgtaata tattactttt aaaagtgact ttttaaaaat gtaaataaaa aagtaactat
     5941 aaatgtgtaa tgcatgttat ttctgtgggt ttttaatttt catctcaact gggcttacaa
     6001 ttttaacata ataaaaatca aatcaaatca aatcaggccc ttatttgtca catacactct
     6061 tgtatactga aataaactaa atgttgagaa aaatttaaat acaccaaaat aacgcatacc
     6121 ttttttgtaa ttacctaagt aaaacattta gttccttttt taagtgactc aagattgtaa
     6181 tctattaatt gtaaaagata aaactagtga tcaatctgac gtgcttctat gcagcctgtg
     6241 agattaattg tgatgtttaa ttattaaaca atttaaatgg atcaaacatt tcacatgtgc
     6301 ttattgtgtc caacaaaacc agcagaagca cttaaaaata ttgcatgaca acatgagttt
     6361 ttaatgtaaa ttatatatac ttcctaaacg aagttaatca tatttctaaa ataataatgt
     6421 cattgaaata tttaaataaa tgataagctt tagttgattc aacataaaat tgaaagcaat
     6481 aaggactttt tttttttaca gcggtgaagc aaaagcatgt aataatcata taatataata
     6541 aataaataaa taaataatgg ctcttggtgc aaatatgaac actttaaata aatatagaat
     6601 aaatatgatc aataagtcat aacgatgtgt tttatgttga ttataattta aacatgtttc
     6661 taacgcaagt tttaaaaact gttttaaagt attattttaa gttataagat caatttgact
     6721 caacattttg tagtgaaaat atgttgtgta aaattaatat aatatacact ataaaaatgc
     6781 agggttccac acaatacatt aatgttgtcc caacacaaat cgattaagtt aacaaatata
     6841 tgtggattga acataaaaaa ttaagttgtc ccaatgaaat ctcaagaatt gtgttgtttc
     6901 agctcatttt aaataagtag ttttaacaag taaaaaataa catatttttg agtgtgtata
     6961 tatatatatt acaaaatata ataatataaa atgtaaaaga tataaaccat gctacatata
     7021 tttaagagct attttgcaca ttactttttt gcaagccatt ttagtgtttt ttttacaaat
     7081 atatattagc taaatataca atatcttaaa tatacaatta atcagacatg caattaatgc
     7141 atttaaccta cataaaaata catgatcaac atatgtttta tgctgattac aactaaatca
     7201 aacgtaatga aattaattta taaaagcgat ttaaatatgt caatatttta agttaaaatc
     7261 acttgaaacc tactataaat gaacttaaat taaaaaaatc tcctttaatt ccaatgcaac
     7321 agtgttgttt tttcagctga ggacccccgg tgcagatggt tggcccccgg cccgctattc
     7381 taaggaagcc tttcttaatt ttagggcggc gctgcatgcg ctcagatcag attcacgggt
     7441 ggagagggtt attttctctt ttttattttt tcttgcctgc tcatatttgg gcctgtgtga
     7501 accccaggct ccgcctcggc ccccaggagc cgctcttatt taaaggggat cctcgccttc
     7561 agtcttcgtc ccagtggaag catcagtctt gtgataacaa cttggtgagt cctacagacc
     7621 agcgctcgac tctaggcttt aaatgcattg atttctctgg attagctacc gggagagttt
     7681 taactttgct tattagtctt gtgctcgaac tgctaggttt aaagagtgtt ttctctaatg
     7741 catgcatgaa gcgacagctg aaaaccagaa atagagcgtt tgtcctcctg ttaaactcta
     7801 gcctttcttt ggcatttgtc agtccgagtc ttcagctgcc ggcaggagat tgggtttcag
     7861 taacacttgc tgagcacaaa ctacacgtgc tttcttcaga tttaattcat tattaatcat
     7921 tctagattta aatgatgttc ttactcattg ctgattattt tagccttgcg gagcagatta
     7981 aatgagcacg tgtttcatta agattgattt aaatataatt aatatttcac cactgtagtt
     8041 ttaaaattgc cttctaagtc atggttgatt tttaataggt atcggctcta attagattac
     8101 tattttggtt tttaaatgtt atttctattt catttgtagg ctcgttttta atgtgatttt
     8161 attagattct agttttaaaa tcatctctta gccttaatga gcagactgaa tgaacatgtt
     8221 tcattaagat taacttcatt ataatgaatt atttaatcat tctagtgggt tataaatcag
     8281 ctctatttaa attaattgtc gtgtttattt ctatttaatt tttgattatt ttttcagggt
     8341 cgtttttatg tgattttttc ccgtttctaa ttttaaacta attgtttgat ttttatatga
     8401 ttcgactctt tttaaatgaa ttgttttgct ttttttattg agcatatata tattatacaa
     8461 tttcaatcaa caattgttgt gctttttaaa tgttgtttct atttcatttc tagggtcgat
     8521 tttattcgat tctagtttta aaatcgtgat ttttaacttc taaccttact gagcagactg
     8581 aatgaacatg tttcatcaag attaacttca ttatagttaa ttattgaatc gtgctagtgg
     8641 tttataaatc agctctattt aaattaatca tcatcattat ttctatttaa tttttattat
     8701 ttctttagga ttatttttat gtgatttttt tcacgttcta gttttaaact caatgctcat
     8761 tactgctcac ccttgctgag cagtctttcg ttaagattaa attaattaat aaccattcta
     8821 gttttaaaac agtgtcttaa ggcatagctg atattttaca agtttactat ttaatatatc
     8881 tcctttcttt ttttagggtc gatttcatct gatttgatta atttttatta tcttcactaa
     8941 gctttttgtt ctaggtttta ttctcagtat tattactgtt atatttgaac tgaatttctc
     9001 ctcatagttt attcatttta tttatttatt ttttgtttat tcgctatttt gtattttagt
     9061 ttttaattag aaattttatt agaatcgttt tgaactagtt tttatgcact ctaacattaa
     9121 tattaaatca taactattat ttttattata atttatttgt gtttatcttt ttgtgtagct
     9181 ttcatgtgtt tttattttag ttttattatg tttgcattga attttttgga ctagtttata
     9241 taacgatata cagaaaagcc atgacaacaa atgtttttat aatacctatt aagtataagc
     9301 taatcagatt tccactattc tgttcaaagt taacattatt agccattctg atggatcatt
     9361 tgacatttgt catatgtctt tatgaacact gctctttcag atgccacttt taaagctgtt
     9421 ttttacagta aatgaacgtt tattattatg acattatttc ctaaggcctc tagagtacag
     9481 caggatgtgg cgtgtgcatt accgtgtaat tatgctgact gtaaatgtgc atttatagac
     9541 tgtcagcatg tctgactgtg cgctgttgtg attggtcctg cacagaccaa g
//
