LOCUS       sequence-221246-        9618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab3da.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab3da
FEATURES             Location/Qualifiers
     source          1..9618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1442..1462)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1470..1486)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1502..1636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1526..1545)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1580..1599
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2075..2262,2263..2274)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2308..2324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2308..2324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2361..2381)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2361..2379)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2400..2416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2413..2435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2424..2440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2448..2454,2455..2472,2473..2478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2493..2514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2631..2648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2802..3390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2882..2901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3561..4352,4353..4421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4184..4203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4422..4526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4689..4710)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5059..5070,5071..5233)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5606..5622
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5642..5662
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcgtcg gccaacgata cccgtcatca
      781 gacacaggtg cagaaagacg ttgctgatca gaattttgac tacatgttca agctgctcat
      841 cattggcaac agcagtgtgg gaaaaacaag ttttctgttc cgttacgctg acgattcgtt
      901 cacctcagca ttcgtcagca cagtcggcat cgacttcaaa gtcaaaacgg tttaccgcaa
      961 cgagaagcga gtcaaactcc agatatggga cacagcggga caggagaggt accgaaccat
     1021 aaccacagct tactacagag gggccatggg atttctgctc atgtatgaca tcactaacca
     1081 agattccttt tacgccgtgc aggactgggc gactcagatt aaaacatact catgggacaa
     1141 cgctcaggtg attctggtgg ggaataaatg tgatctggag gacgacagac tggtggccag
     1201 agaggacgga cagagactgg ccaatgagct aggttttcag ttttttgagg caagtgcgaa
     1261 ggacaacatt aacgtcaagc aggtgtttgg gcgtctggtc gacatcatct gtgacaaaat
     1321 gaacgagagc ttggacacag acccgagcat cttaaccaat cagaaagggc ccagtcttca
     1381 agacacgccc cctgatggac agagcggcgg ctgcggatgt taatgatatt ctttctcaac
     1441 acaactttat tatacatagt tgataattca ctggccgtcg ttttacggta ccatcgatga
     1501 tgatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1561 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1621 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     1681 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     1741 tcctctagat cagatctgcg aagatacggc cacgggtgct cttgatcctg tggctgattt
     1801 tggactgtgc tgctcgcagc tgctgatgaa tcacatactt cctccatttt cttccactga
     1861 ttgactgtta taatttccct aatttccagg tcaaggtgct gtgcattgtg gtaatagatg
     1921 tgacatgacg tcacttccaa aggaccaatg aacatgtctg accaatttca tataatgtga
     1981 aaacgatttt cataggcaga ataaataaca tttaaattaa actgggcatc agcgcaattc
     2041 aattggtttg gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa ataagtactt
     2101 tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa aaaatgtaat
     2161 taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa atccccaaaa
     2221 ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc tctggttctt
     2281 gaccccctac cttcagcaag cccagcagat ccactagttc tagagcggcc gccaccgcgg
     2341 tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     2401 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2461 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2521 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2581 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2641 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2701 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2761 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2821 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2881 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2941 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3001 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3061 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3121 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3181 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3241 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3301 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3361 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3421 gacgctcagt ggaacgaaaa ctcacgttaa ggcattttgg tcatgagatt atcaaaaagg
     3481 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3541 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3601 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3661 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3721 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3781 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3841 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3901 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3961 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4021 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4081 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4141 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4201 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4261 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4321 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4381 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4441 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4501 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     4561 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     4621 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     4681 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     4741 ggcgaaaaac cgtctatcag ggcgatggcc cacgctggct aagaactcat cagcctcccc
     4801 ggtccatcta cccacgtacc aatgcaccaa ttggccacaa tgacggctac tacatggtgc
     4861 cattccttcc tctttatagg aatggagact acctcctgtc caacaatgct cttggatacg
     4921 agtacgccta cctgttggac ccaggtcatt gcacaacacc agaaatgccc tctgatctgc
     4981 aaaagacgtg aatatctgtt cagacaccca tatccactct gttccacaca ggtcagaggt
     5041 ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
     5101 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
     5161 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
     5221 gtaaaagcaa gaaagaaaac tagagattct tgtttaagct tttaatctca aaaaacatta
     5281 aatgaaatgc atacaaggtt ttatcctgct ttagaactgt ttgtatttaa ttatcaaact
     5341 ataagacaga caatctaatg ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa
     5401 cttcagtaga agctgattct caaaattgtt agtgtcaagc ctagctcttt tggggctgaa
     5461 aagcaatcct gcagtgctga aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt
     5521 agtcttgata gagaggctgc aaatagcagg aaacgtgagc agagactccc tggtgtctga
     5581 aacacaggcc agatgggccc tcgagcagga aacagctatg accatgatta cgccaagcta
     5641 tcaactttgt atagaaaagt tggcacgcgc agattccgtg tgggcctggt aatatcttac
     5701 cattttaagc tattttaaga ttagggaaaa aaattaaata aaactacttt aaaattactt
     5761 tccttaaaaa gcaacatatt tatttagttt ttgtttaact ggctcaagat tgtaatattt
     5821 tacttttaca tgttttttta agtacctaaa tgtgttcaaa agtaaagtat gttattgttt
     5881 ttttttttta atgttagcta agctttaaat tgtaatatat tacttttaaa agtgactttt
     5941 taaaaatgta aataaaaaag taactataaa tgtgtaatgc atgttatttc tgtgggtttt
     6001 taattttcat ctcaactggg cttacaattt taacataata aaaatcaaat caaatcaaat
     6061 caggccctta tttgtcacat acactcttgt atactgaaat aaactaaatg ttgagaaaaa
     6121 tttaaataca ccaaaataac gcataccttt tttgtaatta cctaagtaaa acatttagtt
     6181 ccttttttaa gtgactcaag attgtaatct attaattgta aaagataaaa ctagtgatca
     6241 atctgacgtg cttctatgca gcctgtgaga ttaattgtga tgtttaatta ttaaacaatt
     6301 taaatggatc aaacatttca catgtgctta ttgtgtccaa caaaaccagc agaagcactt
     6361 aaaaatattg catgacaaca tgagttttta atgtaaatta tatatacttc ctaaacgaag
     6421 ttaatcatat ttctaaaata ataatgtcat tgaaatattt aaataaatga taagctttag
     6481 ttgattcaac ataaaattga aagcaataag gacttttttt ttttacagcg gtgaagcaaa
     6541 agcatgtaat aatcatataa tataataaat aaataaataa ataatggctc ttggtgcaaa
     6601 tatgaacact ttaaataaat atagaataaa tatgatcaat aagtcataac gatgtgtttt
     6661 atgttgatta taatttaaac atgtttctaa cgcaagtttt aaaaactgtt ttaaagtatt
     6721 attttaagtt ataagatcaa tttgactcaa cattttgtag tgaaaatatg ttgtgtaaaa
     6781 ttaatataat atacactata aaaatgcagg gttccacaca atacattaat gttgtcccaa
     6841 cacaaatcga ttaagttaac aaatatatgt ggattgaaca taaaaaatta agttgtccca
     6901 atgaaatctc aagaattgtg ttgtttcagc tcattttaaa taagtagttt taacaagtaa
     6961 aaaataacat atttttgagt gtgtatatat atatattaca aaatataata atataaaatg
     7021 taaaagatat aaaccatgct acatatattt aagagctatt ttgcacatta cttttttgca
     7081 agccatttta gtgttttttt tacaaatata tattagctaa atatacaata tcttaaatat
     7141 acaattaatc agacatgcaa ttaatgcatt taacctacat aaaaatacat gatcaacata
     7201 tgttttatgc tgattacaac taaatcaaac gtaatgaaat taatttataa aagcgattta
     7261 aatatgtcaa tattttaagt taaaatcact tgaaacctac tataaatgaa cttaaattaa
     7321 aaaaatctcc tttaattcca atgcaacagt gttgtttttt cagctgagga cccccggtgc
     7381 agatggttgg cccccggccc gctattctaa ggaagccttt cttaatttta gggcggcgct
     7441 gcatgcgctc agatcagatt cacgggtgga gagggttatt ttctcttttt tattttttct
     7501 tgcctgctca tatttgggcc tgtgtgaacc ccaggctccg cctcggcccc caggagccgc
     7561 tcttatttaa aggggatcct cgccttcagt cttcgtccca gtggaagcat cagtcttgtg
     7621 ataacaactt ggtgagtcct acagaccagc gctcgactct aggctttaaa tgcattgatt
     7681 tctctggatt agctaccggg agagttttaa ctttgcttat tagtcttgtg ctcgaactgc
     7741 taggtttaaa gagtgttttc tctaatgcat gcatgaagcg acagctgaaa accagaaata
     7801 gagcgtttgt cctcctgtta aactctagcc tttctttggc atttgtcagt ccgagtcttc
     7861 agctgccggc aggagattgg gtttcagtaa cacttgctga gcacaaacta cacgtgcttt
     7921 cttcagattt aattcattat taatcattct agatttaaat gatgttctta ctcattgctg
     7981 attattttag ccttgcggag cagattaaat gagcacgtgt ttcattaaga ttgatttaaa
     8041 tataattaat atttcaccac tgtagtttta aaattgcctt ctaagtcatg gttgattttt
     8101 aataggtatc ggctctaatt agattactat tttggttttt aaatgttatt tctatttcat
     8161 ttgtaggctc gtttttaatg tgattttatt agattctagt tttaaaatca tctcttagcc
     8221 ttaatgagca gactgaatga acatgtttca ttaagattaa cttcattata atgaattatt
     8281 taatcattct agtgggttat aaatcagctc tatttaaatt aattgtcgtg tttatttcta
     8341 tttaattttt gattattttt tcagggtcgt ttttatgtga ttttttcccg tttctaattt
     8401 taaactaatt gtttgatttt tatatgattc gactcttttt aaatgaattg ttttgctttt
     8461 tttattgagc atatatatat tatacaattt caatcaacaa ttgttgtgct ttttaaatgt
     8521 tgtttctatt tcatttctag ggtcgatttt attcgattct agttttaaaa tcgtgatttt
     8581 taacttctaa ccttactgag cagactgaat gaacatgttt catcaagatt aacttcatta
     8641 tagttaatta ttgaatcgtg ctagtggttt ataaatcagc tctatttaaa ttaatcatca
     8701 tcattatttc tatttaattt ttattatttc tttaggatta tttttatgtg atttttttca
     8761 cgttctagtt ttaaactcaa tgctcattac tgctcaccct tgctgagcag tctttcgtta
     8821 agattaaatt aattaataac cattctagtt ttaaaacagt gtcttaaggc atagctgata
     8881 ttttacaagt ttactattta atatatctcc tttctttttt tagggtcgat ttcatctgat
     8941 ttgattaatt tttattatct tcactaagct ttttgttcta ggttttattc tcagtattat
     9001 tactgttata tttgaactga atttctcctc atagtttatt cattttattt atttattttt
     9061 tgtttattcg ctattttgta ttttagtttt taattagaaa ttttattaga atcgttttga
     9121 actagttttt atgcactcta acattaatat taaatcataa ctattatttt tattataatt
     9181 tatttgtgtt tatctttttg tgtagctttc atgtgttttt attttagttt tattatgttt
     9241 gcattgaatt ttttggacta gtttatataa cgatatacag aaaagccatg acaacaaatg
     9301 tttttataat acctattaag tataagctaa tcagatttcc actattctgt tcaaagttaa
     9361 cattattagc cattctgatg gatcatttga catttgtcat atgtctttat gaacactgct
     9421 ctttcagatg ccacttttaa agctgttttt tacagtaaat gaacgtttat tattatgaca
     9481 ttatttccta aggcctctag agtacagcag gatgtggcgt gtgcattacc gtgtaattat
     9541 gctgactgta aatgtgcatt tatagactgt cagcatgtct gactgtgcgc tgttgtgatt
     9601 ggtcctgcac agaccaag
//
