LOCUS       sequence-221252-        9607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab3db.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab3db
FEATURES             Location/Qualifiers
     source          1..9607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1431..1451)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1459..1475)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1491..1625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1515..1534)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1569..1588
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2064..2251,2252..2263)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2297..2313)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2297..2313)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2350..2370)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2350..2368)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2389..2405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2402..2424)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2413..2429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2437..2443,2444..2461,2462..2467))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2482..2503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2620..2637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2791..3379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2871..2890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3550..4341,4342..4410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4173..4192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4411..4515)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4678..4699)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5048..5059,5060..5222)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5595..5611
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5631..5651
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcgtca gtgactgact cgcgtcttca
      781 gccgtcacag aaagatgctg cagaccaaaa ctttgactac atgtttaaac tcctcatcat
      841 cggaaacagc agcgtcggta aaaccagctt tctgtttcga tacgcggacg attccttcac
      901 ctcagccttt gtcagcacag tggggatcga ctttaaggtc aaaaccgttt ttaggaacaa
      961 caagaggatt aaactacaaa tctgggacac ggcgggacag gagcgctaca ggaccatcac
     1021 cacagcctat tacagaggag caatgggctt cctgctcatg ttcgacatca ccaaccggga
     1081 ctcattcaat gctgttcggg actgggccac gcagatcaag acgtactcgt gggacaatgc
     1141 tcaggtgatt ctggtgggaa acaagtgtga tctggaggac gagcggctca ttcctactga
     1201 ggacagccag agactcgcac acgagctcgg tttccagttt ttcgaggcga gcgccaaaga
     1261 cagcatcaac gtgaagcagg tgtttgagtg tctggtggac gtgatctgtg acaagatgac
     1321 cgaaagcctg gacggagacg ccgccgtgtc caaccataaa gactgcagtc tgcaggacgc
     1381 ggctgatgaa ggacacagtg cctgcgcctg ctgaaagcac acaacagaaa caactttatt
     1441 atacatagtt gataattcac tggccgtcgt tttacggtac catcgatgat gatccagaca
     1501 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1561 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1621 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1681 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc
     1741 agatctgcga agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct
     1801 gctcgcagct gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat
     1861 aatttcccta atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt
     1921 cacttccaaa ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc
     1981 ataggcagaa taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg
     2041 taatagcaag ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa
     2101 ataaaattgt aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt
     2161 aaaagtattg atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa
     2221 gtacagtaat caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc
     2281 ttcagcaagc ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag
     2341 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     2401 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2461 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2521 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2581 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2641 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2701 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2761 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2821 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2881 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2941 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3001 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3061 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3121 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3181 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3241 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3301 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3361 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3421 gaacgaaaac tcacgttaag gcattttggt catgagatta tcaaaaagga tcttcaccta
     3481 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3541 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3601 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3661 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3721 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3781 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3841 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3901 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3961 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4021 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4081 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4141 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4201 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4261 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4321 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4381 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4441 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4501 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     4561 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4621 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     4681 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     4741 gtctatcagg gcgatggccc acgctggcta agaactcatc agcctccccg gtccatctac
     4801 ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc attccttcct
     4861 ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga gtacgcctac
     4921 ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga
     4981 atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag
     5041 ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt
     5101 agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa
     5161 gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag
     5221 aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca
     5281 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
     5341 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     5401 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     5461 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     5521 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     5581 gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat caactttgta
     5641 tagaaaagtt ggcacgcgca gattccgtgt gggcctggta atatcttacc attttaagct
     5701 attttaagat tagggaaaaa aattaaataa aactacttta aaattacttt ccttaaaaag
     5761 caacatattt atttagtttt tgtttaactg gctcaagatt gtaatatttt acttttacat
     5821 gtttttttaa gtacctaaat gtgttcaaaa gtaaagtatg ttattgtttt ttttttttaa
     5881 tgttagctaa gctttaaatt gtaatatatt acttttaaaa gtgacttttt aaaaatgtaa
     5941 ataaaaaagt aactataaat gtgtaatgca tgttatttct gtgggttttt aattttcatc
     6001 tcaactgggc ttacaatttt aacataataa aaatcaaatc aaatcaaatc aggcccttat
     6061 ttgtcacata cactcttgta tactgaaata aactaaatgt tgagaaaaat ttaaatacac
     6121 caaaataacg catacctttt ttgtaattac ctaagtaaaa catttagttc cttttttaag
     6181 tgactcaaga ttgtaatcta ttaattgtaa aagataaaac tagtgatcaa tctgacgtgc
     6241 ttctatgcag cctgtgagat taattgtgat gtttaattat taaacaattt aaatggatca
     6301 aacatttcac atgtgcttat tgtgtccaac aaaaccagca gaagcactta aaaatattgc
     6361 atgacaacat gagtttttaa tgtaaattat atatacttcc taaacgaagt taatcatatt
     6421 tctaaaataa taatgtcatt gaaatattta aataaatgat aagctttagt tgattcaaca
     6481 taaaattgaa agcaataagg actttttttt tttacagcgg tgaagcaaaa gcatgtaata
     6541 atcatataat ataataaata aataaataaa taatggctct tggtgcaaat atgaacactt
     6601 taaataaata tagaataaat atgatcaata agtcataacg atgtgtttta tgttgattat
     6661 aatttaaaca tgtttctaac gcaagtttta aaaactgttt taaagtatta ttttaagtta
     6721 taagatcaat ttgactcaac attttgtagt gaaaatatgt tgtgtaaaat taatataata
     6781 tacactataa aaatgcaggg ttccacacaa tacattaatg ttgtcccaac acaaatcgat
     6841 taagttaaca aatatatgtg gattgaacat aaaaaattaa gttgtcccaa tgaaatctca
     6901 agaattgtgt tgtttcagct cattttaaat aagtagtttt aacaagtaaa aaataacata
     6961 tttttgagtg tgtatatata tatattacaa aatataataa tataaaatgt aaaagatata
     7021 aaccatgcta catatattta agagctattt tgcacattac ttttttgcaa gccattttag
     7081 tgtttttttt acaaatatat attagctaaa tatacaatat cttaaatata caattaatca
     7141 gacatgcaat taatgcattt aacctacata aaaatacatg atcaacatat gttttatgct
     7201 gattacaact aaatcaaacg taatgaaatt aatttataaa agcgatttaa atatgtcaat
     7261 attttaagtt aaaatcactt gaaacctact ataaatgaac ttaaattaaa aaaatctcct
     7321 ttaattccaa tgcaacagtg ttgttttttc agctgaggac ccccggtgca gatggttggc
     7381 ccccggcccg ctattctaag gaagcctttc ttaattttag ggcggcgctg catgcgctca
     7441 gatcagattc acgggtggag agggttattt tctctttttt attttttctt gcctgctcat
     7501 atttgggcct gtgtgaaccc caggctccgc ctcggccccc aggagccgct cttatttaaa
     7561 ggggatcctc gccttcagtc ttcgtcccag tggaagcatc agtcttgtga taacaacttg
     7621 gtgagtccta cagaccagcg ctcgactcta ggctttaaat gcattgattt ctctggatta
     7681 gctaccggga gagttttaac tttgcttatt agtcttgtgc tcgaactgct aggtttaaag
     7741 agtgttttct ctaatgcatg catgaagcga cagctgaaaa ccagaaatag agcgtttgtc
     7801 ctcctgttaa actctagcct ttctttggca tttgtcagtc cgagtcttca gctgccggca
     7861 ggagattggg tttcagtaac acttgctgag cacaaactac acgtgctttc ttcagattta
     7921 attcattatt aatcattcta gatttaaatg atgttcttac tcattgctga ttattttagc
     7981 cttgcggagc agattaaatg agcacgtgtt tcattaagat tgatttaaat ataattaata
     8041 tttcaccact gtagttttaa aattgccttc taagtcatgg ttgattttta ataggtatcg
     8101 gctctaatta gattactatt ttggttttta aatgttattt ctatttcatt tgtaggctcg
     8161 tttttaatgt gattttatta gattctagtt ttaaaatcat ctcttagcct taatgagcag
     8221 actgaatgaa catgtttcat taagattaac ttcattataa tgaattattt aatcattcta
     8281 gtgggttata aatcagctct atttaaatta attgtcgtgt ttatttctat ttaatttttg
     8341 attatttttt cagggtcgtt tttatgtgat tttttcccgt ttctaatttt aaactaattg
     8401 tttgattttt atatgattcg actcttttta aatgaattgt tttgcttttt ttattgagca
     8461 tatatatatt atacaatttc aatcaacaat tgttgtgctt tttaaatgtt gtttctattt
     8521 catttctagg gtcgatttta ttcgattcta gttttaaaat cgtgattttt aacttctaac
     8581 cttactgagc agactgaatg aacatgtttc atcaagatta acttcattat agttaattat
     8641 tgaatcgtgc tagtggttta taaatcagct ctatttaaat taatcatcat cattatttct
     8701 atttaatttt tattatttct ttaggattat ttttatgtga tttttttcac gttctagttt
     8761 taaactcaat gctcattact gctcaccctt gctgagcagt ctttcgttaa gattaaatta
     8821 attaataacc attctagttt taaaacagtg tcttaaggca tagctgatat tttacaagtt
     8881 tactatttaa tatatctcct ttcttttttt agggtcgatt tcatctgatt tgattaattt
     8941 ttattatctt cactaagctt tttgttctag gttttattct cagtattatt actgttatat
     9001 ttgaactgaa tttctcctca tagtttattc attttattta tttatttttt gtttattcgc
     9061 tattttgtat tttagttttt aattagaaat tttattagaa tcgttttgaa ctagttttta
     9121 tgcactctaa cattaatatt aaatcataac tattattttt attataattt atttgtgttt
     9181 atctttttgt gtagctttca tgtgttttta ttttagtttt attatgtttg cattgaattt
     9241 tttggactag tttatataac gatatacaga aaagccatga caacaaatgt ttttataata
     9301 cctattaagt ataagctaat cagatttcca ctattctgtt caaagttaac attattagcc
     9361 attctgatgg atcatttgac atttgtcata tgtctttatg aacactgctc tttcagatgc
     9421 cacttttaaa gctgtttttt acagtaaatg aacgtttatt attatgacat tatttcctaa
     9481 ggcctctaga gtacagcagg atgtggcgtg tgcattaccg tgtaattatg ctgactgtaa
     9541 atgtgcattt atagactgtc agcatgtctg actgtgcgct gttgtgattg gtcctgcaca
     9601 gaccaag
//
