LOCUS       sequence-221253-        9792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab40b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab40b
FEATURES             Location/Qualifiers
     source          1..9792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1616..1636)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1644..1660)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1676..1810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1700..1719)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1754..1773
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2249..2436,2437..2448)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2482..2498)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2482..2498)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2535..2555)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2535..2553)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2574..2590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2587..2609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2598..2614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2622..2628,2629..2646,2647..2652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2667..2688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2805..2822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2976..3564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3056..3075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3735..4526,4527..4595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4358..4377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4596..4700)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4863..4884)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5233..5244,5245..5407)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5780..5796
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5816..5836
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgggtcat cgggctgatg tgaagatgaa
      781 ccacaggagc agcccagcaa aagcttatga ttttcttctg aaattcctac tggttggaga
      841 cagtgatgtg gggaaagggg aaatactggc cagtcttcag gatgggtcca gtgagtcacc
      901 ctatggatac aacatgggaa tcgactacaa gactactacc attctcctgg atggacgcag
      961 ggttaagctt caactgtggg acacctcagg acaaggcaga ttctgtacca tattccgctc
     1021 ctattccaga ggagcacagg gagtcatcct tgtttatgac atcacaaacc ggtggtcttt
     1081 cgacggcatc gacagatgga ttaaagagat tgatgagcat gcaccaggtg tccccaagat
     1141 cctggtgggc aacaggcttc atctggccta taagcgtcag gtgaccaccg aacatgcgca
     1201 ggcctttgca gagcgattgg gcgtcacgtt ttttgaggtc agccctctct gtaacttcaa
     1261 catcaccgag tccttcactg aactggcccg cattgtgctc atgaggcacg gcatggagcg
     1321 actctggaga cccaataaag tgttgagtct gcaggacctg tgctgccgtt ctatcgtttc
     1381 ctgcactcca gtgcatctcg tggacaaact tccccttcct gttgccttaa agagtcatct
     1441 gaagtccttt tccatggcta acggcctggt caacaccagg atgatgcacg gccgttccta
     1501 ttcggtcata cctagcagtg ctaaaaaacg gaacggaacc aaaaaatcca aaatcatcta
     1561 tcctccactg agcccatccc agcactgcgc acggacaagc tgcaagattt catagcaact
     1621 ttattataca tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatcc
     1681 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     1741 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1801 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1861 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     1921 agatcagatc tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact
     1981 gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact
     2041 gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat
     2101 gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga
     2161 ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg
     2221 tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac
     2281 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
     2341 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
     2401 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc
     2461 ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc
     2521 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     2581 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2641 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2701 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2881 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2941 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3001 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3061 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3121 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3181 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3241 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3301 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3361 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3421 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3481 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3541 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3601 cagtggaacg aaaactcacg ttaaggcatt ttggtcatga gattatcaaa aaggatcttc
     3661 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3721 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3781 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3841 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3901 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3961 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4021 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4081 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4141 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4201 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4261 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4321 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4381 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4441 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4501 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4561 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4621 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4681 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     4741 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4801 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4861 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     4921 aaaccgtcta tcagggcgat ggcccacgct ggctaagaac tcatcagcct ccccggtcca
     4981 tctacccacg taccaatgca ccaattggcc acaatgacgg ctactacatg gtgccattcc
     5041 ttcctcttta taggaatgga gactacctcc tgtccaacaa tgctcttgga tacgagtacg
     5101 cctacctgtt ggacccaggt cattgcacaa caccagaaat gccctctgat ctgcaaaaga
     5161 cgtgaatatc tgttcagaca cccatatcca ctctgttcca cacaggtcag aggtttgtcc
     5221 aggagttctt gacagaggtg taaaaagtac tcaaaaattt tactcaagtg aaagtacaag
     5281 tacttaggga aaattttact caattaaaag taaaagtatc tggctagaat cttacttgag
     5341 taaaagtaaa aaagtactcc attaaaattg tacttgagta ttaaggaagt aaaagtaaaa
     5401 gcaagaaaga aaactagaga ttcttgttta agcttttaat ctcaaaaaac attaaatgaa
     5461 atgcatacaa ggttttatcc tgctttagaa ctgtttgtat ttaattatca aactataaga
     5521 cagacaatct aatgccagta cacgctactc aaagttgtaa aacctcagat ttaacttcag
     5581 tagaagctga ttctcaaaat tgttagtgtc aagcctagct cttttggggc tgaaaagcaa
     5641 tcctgcagtg ctgaaaagcc tctcacaggc agccgatgcg ggaagaggtg tattagtctt
     5701 gatagagagg ctgcaaatag caggaaacgt gagcagagac tccctggtgt ctgaaacaca
     5761 ggccagatgg gccctcgagc aggaaacagc tatgaccatg attacgccaa gctatcaact
     5821 ttgtatagaa aagttggcac gcgcagattc cgtgtgggcc tggtaatatc ttaccatttt
     5881 aagctatttt aagattaggg aaaaaaatta aataaaacta ctttaaaatt actttcctta
     5941 aaaagcaaca tatttattta gtttttgttt aactggctca agattgtaat attttacttt
     6001 tacatgtttt tttaagtacc taaatgtgtt caaaagtaaa gtatgttatt gttttttttt
     6061 tttaatgtta gctaagcttt aaattgtaat atattacttt taaaagtgac tttttaaaaa
     6121 tgtaaataaa aaagtaacta taaatgtgta atgcatgtta tttctgtggg tttttaattt
     6181 tcatctcaac tgggcttaca attttaacat aataaaaatc aaatcaaatc aaatcaggcc
     6241 cttatttgtc acatacactc ttgtatactg aaataaacta aatgttgaga aaaatttaaa
     6301 tacaccaaaa taacgcatac cttttttgta attacctaag taaaacattt agttcctttt
     6361 ttaagtgact caagattgta atctattaat tgtaaaagat aaaactagtg atcaatctga
     6421 cgtgcttcta tgcagcctgt gagattaatt gtgatgttta attattaaac aatttaaatg
     6481 gatcaaacat ttcacatgtg cttattgtgt ccaacaaaac cagcagaagc acttaaaaat
     6541 attgcatgac aacatgagtt tttaatgtaa attatatata cttcctaaac gaagttaatc
     6601 atatttctaa aataataatg tcattgaaat atttaaataa atgataagct ttagttgatt
     6661 caacataaaa ttgaaagcaa taaggacttt ttttttttac agcggtgaag caaaagcatg
     6721 taataatcat ataatataat aaataaataa ataaataatg gctcttggtg caaatatgaa
     6781 cactttaaat aaatatagaa taaatatgat caataagtca taacgatgtg ttttatgttg
     6841 attataattt aaacatgttt ctaacgcaag ttttaaaaac tgttttaaag tattatttta
     6901 agttataaga tcaatttgac tcaacatttt gtagtgaaaa tatgttgtgt aaaattaata
     6961 taatatacac tataaaaatg cagggttcca cacaatacat taatgttgtc ccaacacaaa
     7021 tcgattaagt taacaaatat atgtggattg aacataaaaa attaagttgt cccaatgaaa
     7081 tctcaagaat tgtgttgttt cagctcattt taaataagta gttttaacaa gtaaaaaata
     7141 acatattttt gagtgtgtat atatatatat tacaaaatat aataatataa aatgtaaaag
     7201 atataaacca tgctacatat atttaagagc tattttgcac attacttttt tgcaagccat
     7261 tttagtgttt tttttacaaa tatatattag ctaaatatac aatatcttaa atatacaatt
     7321 aatcagacat gcaattaatg catttaacct acataaaaat acatgatcaa catatgtttt
     7381 atgctgatta caactaaatc aaacgtaatg aaattaattt ataaaagcga tttaaatatg
     7441 tcaatatttt aagttaaaat cacttgaaac ctactataaa tgaacttaaa ttaaaaaaat
     7501 ctcctttaat tccaatgcaa cagtgttgtt ttttcagctg aggacccccg gtgcagatgg
     7561 ttggcccccg gcccgctatt ctaaggaagc ctttcttaat tttagggcgg cgctgcatgc
     7621 gctcagatca gattcacggg tggagagggt tattttctct tttttatttt ttcttgcctg
     7681 ctcatatttg ggcctgtgtg aaccccaggc tccgcctcgg cccccaggag ccgctcttat
     7741 ttaaagggga tcctcgcctt cagtcttcgt cccagtggaa gcatcagtct tgtgataaca
     7801 acttggtgag tcctacagac cagcgctcga ctctaggctt taaatgcatt gatttctctg
     7861 gattagctac cgggagagtt ttaactttgc ttattagtct tgtgctcgaa ctgctaggtt
     7921 taaagagtgt tttctctaat gcatgcatga agcgacagct gaaaaccaga aatagagcgt
     7981 ttgtcctcct gttaaactct agcctttctt tggcatttgt cagtccgagt cttcagctgc
     8041 cggcaggaga ttgggtttca gtaacacttg ctgagcacaa actacacgtg ctttcttcag
     8101 atttaattca ttattaatca ttctagattt aaatgatgtt cttactcatt gctgattatt
     8161 ttagccttgc ggagcagatt aaatgagcac gtgtttcatt aagattgatt taaatataat
     8221 taatatttca ccactgtagt tttaaaattg ccttctaagt catggttgat ttttaatagg
     8281 tatcggctct aattagatta ctattttggt ttttaaatgt tatttctatt tcatttgtag
     8341 gctcgttttt aatgtgattt tattagattc tagttttaaa atcatctctt agccttaatg
     8401 agcagactga atgaacatgt ttcattaaga ttaacttcat tataatgaat tatttaatca
     8461 ttctagtggg ttataaatca gctctattta aattaattgt cgtgtttatt tctatttaat
     8521 ttttgattat tttttcaggg tcgtttttat gtgatttttt cccgtttcta attttaaact
     8581 aattgtttga tttttatatg attcgactct ttttaaatga attgttttgc tttttttatt
     8641 gagcatatat atattataca atttcaatca acaattgttg tgctttttaa atgttgtttc
     8701 tatttcattt ctagggtcga ttttattcga ttctagtttt aaaatcgtga tttttaactt
     8761 ctaaccttac tgagcagact gaatgaacat gtttcatcaa gattaacttc attatagtta
     8821 attattgaat cgtgctagtg gtttataaat cagctctatt taaattaatc atcatcatta
     8881 tttctattta atttttatta tttctttagg attattttta tgtgattttt ttcacgttct
     8941 agttttaaac tcaatgctca ttactgctca cccttgctga gcagtctttc gttaagatta
     9001 aattaattaa taaccattct agttttaaaa cagtgtctta aggcatagct gatattttac
     9061 aagtttacta tttaatatat ctcctttctt tttttagggt cgatttcatc tgatttgatt
     9121 aatttttatt atcttcacta agctttttgt tctaggtttt attctcagta ttattactgt
     9181 tatatttgaa ctgaatttct cctcatagtt tattcatttt atttatttat tttttgttta
     9241 ttcgctattt tgtattttag tttttaatta gaaattttat tagaatcgtt ttgaactagt
     9301 ttttatgcac tctaacatta atattaaatc ataactatta tttttattat aatttatttg
     9361 tgtttatctt tttgtgtagc tttcatgtgt ttttatttta gttttattat gtttgcattg
     9421 aattttttgg actagtttat ataacgatat acagaaaagc catgacaaca aatgttttta
     9481 taatacctat taagtataag ctaatcagat ttccactatt ctgttcaaag ttaacattat
     9541 tagccattct gatggatcat ttgacatttg tcatatgtct ttatgaacac tgctctttca
     9601 gatgccactt ttaaagctgt tttttacagt aaatgaacgt ttattattat gacattattt
     9661 cctaaggcct ctagagtaca gcaggatgtg gcgtgtgcat taccgtgtaa ttatgctgac
     9721 tgtaaatgtg catttataga ctgtcagcat gtctgactgt gcgctgttgt gattggtcct
     9781 gcacagacca ag
//
