LOCUS       sequence-221255-        9602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab42b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab42b
FEATURES             Location/Qualifiers
     source          1..9602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1426..1446)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1454..1470)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1486..1620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1510..1529)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1564..1583
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2059..2246,2247..2258)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2292..2308)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2292..2308)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2345..2365)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2345..2363)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2384..2400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2397..2419)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2408..2424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2432..2438,2439..2456,2457..2462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2477..2498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2615..2632)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2786..3374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2866..2885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3545..4336,4337..4405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4168..4187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4406..4510)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4673..4694)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5043..5054,5055..5217)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5590..5606
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5626..5646
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggatctc acattgtggc agtaccagtt
      781 tcggatcatc atgcttggag actctacagt tggaaagtcg tccatgttaa agcgctacac
      841 tgaggacttg tttctggaat gcatcaatca gactgtcgga gtggacttct atgtacactt
      901 cctagaggtg gaacctgggg tgagggttaa actgcagttt tgggacacag ctggtcaaga
      961 gaggtttagg tctgtgactc gatcctacta ccgtaactct gttggaggtc tcctggtgtt
     1021 cgatctgggc aatcgcaaat cttttgaaaa tgtgcgggaa tggtacgccg aagtgtgtga
     1081 acgtgtgcat ccctacactg tgctcttcgt cctggtagga cacaagagcg accgcgtcaa
     1141 aggtggagaa agagccgtgg accgaactga agcagaaaag ttggcgagcc agctgggagc
     1201 cccttacatc gaggcctcgg ccaaaacggg tcacaatgtg aaagaagcct tcgacctctt
     1261 gacccggcgg atttatcagg gcttaaaaag tggtgagatc cagttgcgcg aagggtggga
     1321 tggcgtcaaa agctcagcgc ctacagccca aaccctccag aagttacaaa acactgaatc
     1381 tgctgagaaa aagagctgta actgttaact attacaacac gtggccaact ttattataca
     1441 tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatcc agacatgata
     1501 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1561 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1621 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1681 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc
     1741 tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact gtgctgctcg
     1801 cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact gttataattt
     1861 ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat gacgtcactt
     1921 ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga ttttcatagg
     1981 cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg tttggtaata
     2041 gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac tgtaaataaa
     2101 attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta aaagtaaaag
     2161 tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata cttaagtaca
     2221 gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc ctaccttcag
     2281 caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt
     2341 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     2401 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2461 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2521 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2581 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3421 aaaactcacg ttaaggcatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3541 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3601 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3661 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3721 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3781 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3841 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3901 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3961 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4021 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4081 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4141 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4201 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4261 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4321 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4381 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4441 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4501 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     4561 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4621 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4681 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4741 tcagggcgat ggcccacgct ggctaagaac tcatcagcct ccccggtcca tctacccacg
     4801 taccaatgca ccaattggcc acaatgacgg ctactacatg gtgccattcc ttcctcttta
     4861 taggaatgga gactacctcc tgtccaacaa tgctcttgga tacgagtacg cctacctgtt
     4921 ggacccaggt cattgcacaa caccagaaat gccctctgat ctgcaaaaga cgtgaatatc
     4981 tgttcagaca cccatatcca ctctgttcca cacaggtcag aggtttgtcc aggagttctt
     5041 gacagaggtg taaaaagtac tcaaaaattt tactcaagtg aaagtacaag tacttaggga
     5101 aaattttact caattaaaag taaaagtatc tggctagaat cttacttgag taaaagtaaa
     5161 aaagtactcc attaaaattg tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga
     5221 aaactagaga ttcttgttta agcttttaat ctcaaaaaac attaaatgaa atgcatacaa
     5281 ggttttatcc tgctttagaa ctgtttgtat ttaattatca aactataaga cagacaatct
     5341 aatgccagta cacgctactc aaagttgtaa aacctcagat ttaacttcag tagaagctga
     5401 ttctcaaaat tgttagtgtc aagcctagct cttttggggc tgaaaagcaa tcctgcagtg
     5461 ctgaaaagcc tctcacaggc agccgatgcg ggaagaggtg tattagtctt gatagagagg
     5521 ctgcaaatag caggaaacgt gagcagagac tccctggtgt ctgaaacaca ggccagatgg
     5581 gccctcgagc aggaaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa
     5641 aagttggcac gcgcagattc cgtgtgggcc tggtaatatc ttaccatttt aagctatttt
     5701 aagattaggg aaaaaaatta aataaaacta ctttaaaatt actttcctta aaaagcaaca
     5761 tatttattta gtttttgttt aactggctca agattgtaat attttacttt tacatgtttt
     5821 tttaagtacc taaatgtgtt caaaagtaaa gtatgttatt gttttttttt tttaatgtta
     5881 gctaagcttt aaattgtaat atattacttt taaaagtgac tttttaaaaa tgtaaataaa
     5941 aaagtaacta taaatgtgta atgcatgtta tttctgtggg tttttaattt tcatctcaac
     6001 tgggcttaca attttaacat aataaaaatc aaatcaaatc aaatcaggcc cttatttgtc
     6061 acatacactc ttgtatactg aaataaacta aatgttgaga aaaatttaaa tacaccaaaa
     6121 taacgcatac cttttttgta attacctaag taaaacattt agttcctttt ttaagtgact
     6181 caagattgta atctattaat tgtaaaagat aaaactagtg atcaatctga cgtgcttcta
     6241 tgcagcctgt gagattaatt gtgatgttta attattaaac aatttaaatg gatcaaacat
     6301 ttcacatgtg cttattgtgt ccaacaaaac cagcagaagc acttaaaaat attgcatgac
     6361 aacatgagtt tttaatgtaa attatatata cttcctaaac gaagttaatc atatttctaa
     6421 aataataatg tcattgaaat atttaaataa atgataagct ttagttgatt caacataaaa
     6481 ttgaaagcaa taaggacttt ttttttttac agcggtgaag caaaagcatg taataatcat
     6541 ataatataat aaataaataa ataaataatg gctcttggtg caaatatgaa cactttaaat
     6601 aaatatagaa taaatatgat caataagtca taacgatgtg ttttatgttg attataattt
     6661 aaacatgttt ctaacgcaag ttttaaaaac tgttttaaag tattatttta agttataaga
     6721 tcaatttgac tcaacatttt gtagtgaaaa tatgttgtgt aaaattaata taatatacac
     6781 tataaaaatg cagggttcca cacaatacat taatgttgtc ccaacacaaa tcgattaagt
     6841 taacaaatat atgtggattg aacataaaaa attaagttgt cccaatgaaa tctcaagaat
     6901 tgtgttgttt cagctcattt taaataagta gttttaacaa gtaaaaaata acatattttt
     6961 gagtgtgtat atatatatat tacaaaatat aataatataa aatgtaaaag atataaacca
     7021 tgctacatat atttaagagc tattttgcac attacttttt tgcaagccat tttagtgttt
     7081 tttttacaaa tatatattag ctaaatatac aatatcttaa atatacaatt aatcagacat
     7141 gcaattaatg catttaacct acataaaaat acatgatcaa catatgtttt atgctgatta
     7201 caactaaatc aaacgtaatg aaattaattt ataaaagcga tttaaatatg tcaatatttt
     7261 aagttaaaat cacttgaaac ctactataaa tgaacttaaa ttaaaaaaat ctcctttaat
     7321 tccaatgcaa cagtgttgtt ttttcagctg aggacccccg gtgcagatgg ttggcccccg
     7381 gcccgctatt ctaaggaagc ctttcttaat tttagggcgg cgctgcatgc gctcagatca
     7441 gattcacggg tggagagggt tattttctct tttttatttt ttcttgcctg ctcatatttg
     7501 ggcctgtgtg aaccccaggc tccgcctcgg cccccaggag ccgctcttat ttaaagggga
     7561 tcctcgcctt cagtcttcgt cccagtggaa gcatcagtct tgtgataaca acttggtgag
     7621 tcctacagac cagcgctcga ctctaggctt taaatgcatt gatttctctg gattagctac
     7681 cgggagagtt ttaactttgc ttattagtct tgtgctcgaa ctgctaggtt taaagagtgt
     7741 tttctctaat gcatgcatga agcgacagct gaaaaccaga aatagagcgt ttgtcctcct
     7801 gttaaactct agcctttctt tggcatttgt cagtccgagt cttcagctgc cggcaggaga
     7861 ttgggtttca gtaacacttg ctgagcacaa actacacgtg ctttcttcag atttaattca
     7921 ttattaatca ttctagattt aaatgatgtt cttactcatt gctgattatt ttagccttgc
     7981 ggagcagatt aaatgagcac gtgtttcatt aagattgatt taaatataat taatatttca
     8041 ccactgtagt tttaaaattg ccttctaagt catggttgat ttttaatagg tatcggctct
     8101 aattagatta ctattttggt ttttaaatgt tatttctatt tcatttgtag gctcgttttt
     8161 aatgtgattt tattagattc tagttttaaa atcatctctt agccttaatg agcagactga
     8221 atgaacatgt ttcattaaga ttaacttcat tataatgaat tatttaatca ttctagtggg
     8281 ttataaatca gctctattta aattaattgt cgtgtttatt tctatttaat ttttgattat
     8341 tttttcaggg tcgtttttat gtgatttttt cccgtttcta attttaaact aattgtttga
     8401 tttttatatg attcgactct ttttaaatga attgttttgc tttttttatt gagcatatat
     8461 atattataca atttcaatca acaattgttg tgctttttaa atgttgtttc tatttcattt
     8521 ctagggtcga ttttattcga ttctagtttt aaaatcgtga tttttaactt ctaaccttac
     8581 tgagcagact gaatgaacat gtttcatcaa gattaacttc attatagtta attattgaat
     8641 cgtgctagtg gtttataaat cagctctatt taaattaatc atcatcatta tttctattta
     8701 atttttatta tttctttagg attattttta tgtgattttt ttcacgttct agttttaaac
     8761 tcaatgctca ttactgctca cccttgctga gcagtctttc gttaagatta aattaattaa
     8821 taaccattct agttttaaaa cagtgtctta aggcatagct gatattttac aagtttacta
     8881 tttaatatat ctcctttctt tttttagggt cgatttcatc tgatttgatt aatttttatt
     8941 atcttcacta agctttttgt tctaggtttt attctcagta ttattactgt tatatttgaa
     9001 ctgaatttct cctcatagtt tattcatttt atttatttat tttttgttta ttcgctattt
     9061 tgtattttag tttttaatta gaaattttat tagaatcgtt ttgaactagt ttttatgcac
     9121 tctaacatta atattaaatc ataactatta tttttattat aatttatttg tgtttatctt
     9181 tttgtgtagc tttcatgtgt ttttatttta gttttattat gtttgcattg aattttttgg
     9241 actagtttat ataacgatat acagaaaagc catgacaaca aatgttttta taatacctat
     9301 taagtataag ctaatcagat ttccactatt ctgttcaaag ttaacattat tagccattct
     9361 gatggatcat ttgacatttg tcatatgtct ttatgaacac tgctctttca gatgccactt
     9421 ttaaagctgt tttttacagt aaatgaacgt ttattattat gacattattt cctaaggcct
     9481 ctagagtaca gcaggatgtg gcgtgtgcat taccgtgtaa ttatgctgac tgtaaatgtg
     9541 catttataga ctgtcagcat gtctgactgt gcgctgttgt gattggtcct gcacagacca
     9601 ag
//
