LOCUS       sequence-221266-       10530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-tagged enzymatically inactive (delta RING) Arkadia in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Mavrakis KJ, Andrew RL, Lee KL, Petropoulou C, Dixon JE, Navaratnam 
            N, Norris DP, Episkopou V
  TITLE     Arkadia enhances Nodal/TGF-beta signaling by coupling 
            phospho-Smad2/3 activity and turnover.
  JOURNAL   PLoS Biol. 2007 Mar;5(3):e67. doi: 10.1371/journal.pbio.0050067.
  PUBMED    17341133
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2007-03"; volume: "5(3):e67. doi"; pages: " 
            10.1371/journal.pbio.0050067"
COMMENT     Sequence Label: gfp-Arkadia/ RNF111 deltaRING
FEATURES             Location/Qualifiers
     source          1..10530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     407..429
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     491..510
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     552..571
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        552..570
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    571..595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        611..720
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             741..758
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             768..812
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     regulatory      840..849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(846..848,849..851,852..1562)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(846..848,849..851,852..1562)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(891..912)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1152..1171)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1499..1520
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3090..3110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             4491..4514
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    4568..5030
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4624..4641)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5069..6088
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5069..6088
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(6253..6272)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6318..6373
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6372..6391)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      6461..6508
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     6521..7226
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           6609..6981
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(6638..7226)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     protein_bind    complement(7235..7268)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(7436..8023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7515..7534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8036..8827,8828..8896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8659..8678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_recomb     9139..9966
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             9139..9263
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(9301..9906)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     enhancer        10098..10401
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
       61 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
      121 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
      181 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
      241 cttctccttc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc
      301 tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc
      361 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
      421 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
      481 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat
      541 cggaccgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attccccgga
      601 gttaatccgg gacctttaat tcaacccaac acaatatatt atagttaaat aagaattatt
      661 atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt atttacaatc
      721 aaaggagata taccatggca caccatcacc accatcactc ttctggtaaa gaaaccgctg
      781 ctgcgaaatt tgaacgccag cacatggact cgccaccgcc ttctggtctg gtccccctcg
      841 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      901 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      961 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1021 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     1081 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1141 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1201 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1261 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1321 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1381 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1441 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1501 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1561 agtccggact cagatctaag cttgagctct acaccctaaa agtggctatg aagagtggga
     1621 ctcctgatgc gcccactaca caggagagtc tgaaggcggt ccttctgcac ccacagcccc
     1681 taggggccac caagagcttc cctgcagaag tggagatgat taatagcaaa gtggggaatg
     1741 aattctctca tctgtgtgat gattctcaaa aacaagagaa ggacatgact ggcaaccagc
     1801 aagaacaaga aaaaagtggt gttgtgagaa aaaagcgcaa aagccagcag gctggcccct
     1861 catatgtgca gaactgtgtc aaagaaaatc aggaaatcct agggcgcaga caacagctag
     1921 agacaccgag tgatgaggat aatgactctt ccttaagtga gtgtctgtct tctccctcat
     1981 ctagtctcca ttttgggggc tctgatacag tgacttcaga tgaggataaa gaagtctcgg
     2041 tgagacatac ccagcctgtt ctgagtgcta aaagtagaag tcacagtgca aggtctcaca
     2101 agtggcctcg aactgaggca gatcctgtgc caagtttgtt aatgaaaaga ccttgttttc
     2161 atggcagtgc actaaggaga gtcacatgca gaaagaggct ggtgaagagc agctcctcgc
     2221 agaggacaca gaagcagaag gagaggatgc tagtgcagag gaagaagcgg gaggcgctag
     2281 ctcagaggaa gtatgctctt ctctccagct ccagtagctc cagtgagaat gacctcagca
     2341 gcgattcctc gtccagctcc tccaccgatg gagaagagga cctgtgtgca tctgccagtg
     2401 agaaccccag caaccccgct gctccctcag gaagtattga tgaagatgtt gtggtgatag
     2461 aagcctcctt cactccccag gtcactgcca atgaagaaat taatgttacc tcaactgaca
     2521 gtgaagtgga gattgttaca gttggagaaa gctatcggtc tcgttcaacc cttggacact
     2581 ccagatctca ttggagccaa ggttcaagtt cgcatacagg acggccacag gagtctcgaa
     2641 accgcagtag gatctctact gttatacagc ccctgaggca gaatgcagca gaagttgtgg
     2701 accttactgt tgatgaagat gaacctacta tagtaccaac cacttctgct agaatggact
     2761 cacaaactac tagtgcttcc attaacaatt caaatccatc tacctctgag caagcctctg
     2821 atactacttc aactgtggcc agtagccagc cttccacagt gtcagagact gaagctactc
     2881 ttacaagcaa tagtgcaact ggttcttctg tgggcgatga cgtgaggaga actgcatcta
     2941 gtgctgtccc agaaagtggt cctcctgcaa tgccaaggct accttcttgc tgcccccagc
     3001 actcaccatg tggagggaca tcacagagcc atcatgcatt agcacaccca cactcaagtt
     3061 gttttcagca gcatggccac cattttcagc atcaccacca tcaccaccat actccccacc
     3121 cagctgtccc agtttctcct tccttcagtg accctgcttg cccagtggaa agacctcaag
     3181 tacaggcgcc ttgtggagca aatagcagct ctggttccag ctaccatgac cagcaggcat
     3241 tgccagtcga tctgagcaac agtgctctca ggactcatgg aagtgggggt ttccatggtg
     3301 catctgcttt tgacccctgc tgccctgtca catcttcccg ggctgcagtc tttggccatc
     3361 aggctgctgc tgccccgact cagccattat caatagatgg ctatggctcc agcatggttg
     3421 cccagcccca gccccagccc cctccacagc cttctctttc gtcatgtcgg cactacatgc
     3481 cacctccgta tgcttccttg acaagaccac tgcaccatca agcctcagct tgccatcact
     3541 ctcatggaaa tgcccctccc cagactcagc ctccccctca agtggattac gtgattccgc
     3601 atcctgtcca tgctttccat tctcagatat cttctcatgc agcatctcac cctgtggcac
     3661 caccgccgcc aacacattta ggcagtacag cggcacccat ccctcagcat cttcctcctg
     3721 ctcaccagcc gatttcacac catattccag ccccagcacc ttcagcccag agactgcacc
     3781 ctcatgaagt gatgcagagg atggaagttc agaggaggag gatgatgcag catccaacgc
     3841 gggcacatga acggcccccg ccccatccac ataggatgca cccaaactat ggccatgggc
     3901 atcatattca tgtgcctcaa accatgtctt cacatcctcg ccaggctcca gagagaactg
     3961 cctgggaact ggggattgaa gctggagtaa ctgcagctac ctacacacct ggtgctctgc
     4021 accctcacct ggcccattac cacgccccgc ctcgacttca tcacctccag ttaggagcac
     4081 ttcctttaat ggttcctgac atggcaggct atcctcacat ccgttatatt tcttcaggat
     4141 tggatggagc atcattcaga ggtcctttta ggggcaattt tgaggaattg attcatctgg
     4201 aagaaagatt aggaaatgtc aatcgtggag cctcccaggg aacaattgaa agatgcacat
     4261 atccacataa atataaaaag gtaacaactg attggttctc acagaggaaa ctgcactgca
     4321 aacaagatgg ggaagaaggg actgaggaag acacagagga aaaatgtact atctgtttgt
     4381 ctattttaga ggaaggtgaa gatgtgaggc gccttccatg tatgcacctt ttccaccaag
     4441 tgtgtgttga tccatatata gggcccgggt tataattacc tcaggtcgag caccaccatc
     4501 accatcacca tcactaagtg attaacctca ggtgcaggct gcctatcaga aggtggtggc
     4561 tggtgtggag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     4621 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     4681 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     4741 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     4801 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctcacct
     4861 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     4921 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     4981 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca
     5041 acccgtacca aagatggata gatccggaaa gcctgaactc accgcgacgt ctgttgagaa
     5101 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
     5161 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
     5221 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
     5281 gattccggaa gtgcttgaca ttggggaatt tagcgagagc ctgacctatt gcatctcccg
     5341 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
     5401 accggtcgcg gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
     5461 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     5521 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     5581 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     5641 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     5701 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     5761 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     5821 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     5881 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     5941 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     6001 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     6061 cagcactcgt ccgagggcaa aggaatagag tagatgccga ccgaacaaga gctgatttcg
     6121 agaacgcctc agccagcaac tcgcgcgagc ctagcaaggc aaatgcgaga gaacggcctt
     6181 acgcttggtg gcacagttct cgtccacagt tcgctaacca atgccctggc tcacaaatac
     6241 cactgagatc gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     6301 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     6361 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     6421 gtatttggtt tagagtttgg caacatatgc ccatatgtaa ctagcataac cccttggggc
     6481 ctctaaacgg gtcttgaggg gttttttgct gaaagcatgc ggaggaaatt ctccttgaag
     6541 tttccctggt gttcaaagta aaggagtttg caccagacgc acctctgttc actggtccgg
     6601 cgtattaaaa cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt
     6661 gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag
     6721 ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata
     6781 ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt
     6841 ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat
     6901 cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt
     6961 cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca
     7021 attgactcga cgtaaacacg ttaaatagag cttggacata tttaacatcg ggcgtgttag
     7081 ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag
     7141 acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca
     7201 aatttgtagt tgagcttttt ggaattgcga tcgcataact tcgtatagca tacattatac
     7261 gaagttataa gctcggaacg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7321 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7381 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7441 ataggctccg ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7861 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgttacc
     8041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8941 gtctcatgtc cgcgcgtttc ctgcatcttt taatcaaatc ccaagatgtg tataaacgcg
     9001 ccggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg
     9061 ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca
     9121 agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa
     9181 actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc
     9241 tcaatcctat ttgtaattat tgaataataa aacaattata aatgtcaaat ttgtttttta
     9301 ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc
     9361 gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg
     9421 cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc
     9481 cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt atccatatat
     9541 gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg
     9601 gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag
     9661 ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc
     9721 ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt
     9781 ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa
     9841 cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac
     9901 agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata
     9961 gttgctgatg gccggccgta atgagacgca caaactaata tcacaaactg gaaatgtcta
    10021 tcaatatata gttgctctag ttattaatag taatcaatta cggggtcatt agttcatagc
    10081 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    10141 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
    10201 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    10261 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
    10321 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
    10381 ttagtcatcg ctattaccat gcatggtcga ggtgagcccc acgttctgct tcactctccc
    10441 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
    10501 agcgatgggg gcgggggggg ggggggggcg
//
