LOCUS       sequence-22127-e         594 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Wang L, Harris TE, Roth RA, Lawrence JC
  TITLE     PRAS40 regulates mTORC1 kinase activity by functioning as a direct 
            inhibitor of substrate binding.
  JOURNAL   J Biol Chem. 2007 Jul 6. 282(27):20036-44.
  PUBMED    17510057
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 6. 282(27):20036-44."
COMMENT     Sequence Label: HA PRAS40 F129A
FEATURES             Location/Qualifiers
     source          1..594
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttagcagagc tctctggcta ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgg taccgagctc
      121 ggatccgcca ccatggcgtc ggggcgcccc gaggagctgt gggaggccgt ggtgggggcc
      181 gctgagcgct tccgggcccg gactggcacg gagctggtgc tgctgaccgc ggccccgccg
      241 ccaccacccc gcccgggccc ctgtgcctat cctgcccatg gtcgaggagc cctggcggag
      301 gcagcgcgcc gttgcctcca cgacatcgca ctggcccaca gggctgccac tgctgctcgg
      361 cctcctgcgc ccccaccagc accacagcca cccagtccca cacccagccc accccggcct
      421 accctggcca gagaggacaa cgaggaggac gaggatgagc ccacagagac agagacctcc
      481 ggggagcagc tgggcattan tgataatgga gggctagctg tgatggatga ggacgccacc
      541 ctccaggacc ttcccccctt ctgtgagtca naccccgaga gtacanatga tggc
//
