LOCUS       sequence-221270-        2845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC19 backbone with S. pyogenes CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Wright AV, Doudna JA
  TITLE     Protecting genome integrity during CRISPR immune adaptation.
  JOURNAL   Nat Struct Mol Biol. 2016 Oct;23(10):876-883. doi: 
            10.1038/nsmb.3289. Epub 2016 Sep 5.
  PUBMED    27595346
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3289";
            journalName: "Nat Struct Mol Biol"; date: "2016-10"; volume: "23"; 
            issue: "10"; pages: "876-883"
COMMENT     Sequence Label: pAW091
FEATURES             Location/Qualifiers
     source          1..2845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..103
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   104..139
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   170..205
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   236..271
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   302..337
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   368..403
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   434..469
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     rep_origin      complement(626..1214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(706..725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1385..2176,2177..2245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2008..2027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2246..2350)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2418..2436
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2474..2496)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2596..2615
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2809..2831
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2823..2840
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2824..2840
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctactcttaa taaatgcagt aatacagggg cttttcaaga ctgaagtcta gctgagacaa
       61 atagtgcgat tacaaaattt tttagacaaa aatagtctac gaggttttag agctatgctg
      121 ttttgaatgg tcccaaaact gcgctggttg atttcttctt gcgctttttg ttttagagct
      181 atgctgtttt gaatggtccc aaaacttata tgaacataac tcaatttgta aaaaagtttt
      241 agagctatgc tgttttgaat ggtcccaaaa caggaatatc cgcaataatt aattgcgctc
      301 tgttttagag ctatgctgtt ttgaatggtc ccaaaacagt gccgaggaaa aattaggtgc
      361 gcttggcgtt ttagagctat gctgttttga atggtcccaa aactaaattt gtttagcagg
      421 taaaccgtgc tttgttttag agctatgctg ttttgaatgg tcccaaaact tcagcacact
      481 gagacttgtt gagttccatg ttttagagct atgctgtttt gaatggtctc cattcaacat
      541 tgccgatgat aacttgagaa agagacatgt gagcaaaagg ccagcaaaag gccaggaacc
      601 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      661 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      721 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      781 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      841 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      901 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      961 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1021 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1081 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1141 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1201 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1261 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1321 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1381 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1441 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1501 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1561 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1621 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1681 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1741 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1801 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1861 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1921 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1981 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2041 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2101 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2161 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2221 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2281 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2341 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2401 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     2461 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2521 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2581 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2641 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2701 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2761 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2821 gttgtaaaac gacggccagt gaatt
//
