LOCUS       sequence-221275-        9586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab4b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab4b
FEATURES             Location/Qualifiers
     source          1..9586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1410..1430)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1438..1454)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1470..1604
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1494..1513)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1548..1567
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2043..2230,2231..2242)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2276..2292)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2276..2292)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2329..2349)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2329..2347)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2368..2384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2381..2403)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2392..2408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2416..2422,2423..2440,2441..2446))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2461..2482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2599..2616)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2770..3358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2850..2869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3529..4320,4321..4389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4152..4171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4390..4494)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4657..4678)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5027..5038,5039..5201)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5574..5590
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5610..5630
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgtcagag acgtacgatt tcctgttcaa
      781 gttcctggtg attggcagcg ccgggactgg gaaatcatgc ctccttcacc agttcataga
      841 gaacaagttc aaacaggact ccaatcacac catcggcgtt gagttcggct ccagggttgt
      901 caatgttggt ggcaaaacag tcaaactgca gatctgggac acggctggac aggagcgctt
      961 taggtcagtg acccgcagtt actatcgtgg agcagcagga gctcttctcg tgtatgacat
     1021 cacaagtcgg gagacctaca atgctctgac taactggttg acagacgcaa ggacactggc
     1081 cagtcccaac atcgtcatca tcctgtgcgg aaataagaag gatctgggcg cagaccgtga
     1141 agtcactttc ctagaggcat ctcgcttcgc ccaggaaaat gagctgatgt ttttagagac
     1201 gagtgctttg actggggaga atgtcgagga agcctttctt aaatgcgctc gcagcatcct
     1261 caacaagatt gaatcaggtg agctggaccc agagcggatg ggttctggga ttcagtatgg
     1321 cgacgcgtcc ctgcggcaga tccgacagcc ccggggctct gctgcacaga ccaagcagca
     1381 gtgtaactgt tagggctctg aagacccttc aactttatta tacatagttg ataattcact
     1441 ggccgtcgtt ttacggtacc atcgatgatg atccagacat gataagatac attgatgagt
     1501 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1561 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1621 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     1681 tctacaaatg tggtatggct gattatgatc ctctagatca gatctgcgaa gatacggcca
     1741 cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc
     1801 acatacttcc tccattttct tccactgatt gactgttata atttccctaa tttccaggtc
     1861 aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa
     1921 catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt
     1981 taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa
     2041 tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa
     2101 agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg
     2161 tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt
     2221 actcaagtac tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc
     2281 actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg
     2341 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2401 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2461 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2521 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2581 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2641 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2701 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2761 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2821 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2881 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2941 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3001 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3061 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3421 cattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4501 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     4561 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     4621 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     4681 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4741 cgctggctaa gaactcatca gcctccccgg tccatctacc cacgtaccaa tgcaccaatt
     4801 ggccacaatg acggctacta catggtgcca ttccttcctc tttataggaa tggagactac
     4861 ctcctgtcca acaatgctct tggatacgag tacgcctacc tgttggaccc aggtcattgc
     4921 acaacaccag aaatgccctc tgatctgcaa aagacgtgaa tatctgttca gacacccata
     4981 tccactctgt tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa
     5041 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta
     5101 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa
     5161 attgtacttg agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg
     5221 tttaagcttt taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt
     5281 agaactgttt gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct
     5341 actcaaagtt gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag
     5401 tgtcaagcct agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac
     5461 aggcagccga tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa
     5521 acgtgagcag agactccctg gtgtctgaaa cacaggccag atgggccctc gagcaggaaa
     5581 cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg gcacgcgcag
     5641 attccgtgtg ggcctggtaa tatcttacca ttttaagcta ttttaagatt agggaaaaaa
     5701 attaaataaa actactttaa aattactttc cttaaaaagc aacatattta tttagttttt
     5761 gtttaactgg ctcaagattg taatatttta cttttacatg tttttttaag tacctaaatg
     5821 tgttcaaaag taaagtatgt tattgttttt tttttttaat gttagctaag ctttaaattg
     5881 taatatatta cttttaaaag tgacttttta aaaatgtaaa taaaaaagta actataaatg
     5941 tgtaatgcat gttatttctg tgggttttta attttcatct caactgggct tacaatttta
     6001 acataataaa aatcaaatca aatcaaatca ggcccttatt tgtcacatac actcttgtat
     6061 actgaaataa actaaatgtt gagaaaaatt taaatacacc aaaataacgc ataccttttt
     6121 tgtaattacc taagtaaaac atttagttcc ttttttaagt gactcaagat tgtaatctat
     6181 taattgtaaa agataaaact agtgatcaat ctgacgtgct tctatgcagc ctgtgagatt
     6241 aattgtgatg tttaattatt aaacaattta aatggatcaa acatttcaca tgtgcttatt
     6301 gtgtccaaca aaaccagcag aagcacttaa aaatattgca tgacaacatg agtttttaat
     6361 gtaaattata tatacttcct aaacgaagtt aatcatattt ctaaaataat aatgtcattg
     6421 aaatatttaa ataaatgata agctttagtt gattcaacat aaaattgaaa gcaataagga
     6481 cttttttttt ttacagcggt gaagcaaaag catgtaataa tcatataata taataaataa
     6541 ataaataaat aatggctctt ggtgcaaata tgaacacttt aaataaatat agaataaata
     6601 tgatcaataa gtcataacga tgtgttttat gttgattata atttaaacat gtttctaacg
     6661 caagttttaa aaactgtttt aaagtattat tttaagttat aagatcaatt tgactcaaca
     6721 ttttgtagtg aaaatatgtt gtgtaaaatt aatataatat acactataaa aatgcagggt
     6781 tccacacaat acattaatgt tgtcccaaca caaatcgatt aagttaacaa atatatgtgg
     6841 attgaacata aaaaattaag ttgtcccaat gaaatctcaa gaattgtgtt gtttcagctc
     6901 attttaaata agtagtttta acaagtaaaa aataacatat ttttgagtgt gtatatatat
     6961 atattacaaa atataataat ataaaatgta aaagatataa accatgctac atatatttaa
     7021 gagctatttt gcacattact tttttgcaag ccattttagt gtttttttta caaatatata
     7081 ttagctaaat atacaatatc ttaaatatac aattaatcag acatgcaatt aatgcattta
     7141 acctacataa aaatacatga tcaacatatg ttttatgctg attacaacta aatcaaacgt
     7201 aatgaaatta atttataaaa gcgatttaaa tatgtcaata ttttaagtta aaatcacttg
     7261 aaacctacta taaatgaact taaattaaaa aaatctcctt taattccaat gcaacagtgt
     7321 tgttttttca gctgaggacc cccggtgcag atggttggcc cccggcccgc tattctaagg
     7381 aagcctttct taattttagg gcggcgctgc atgcgctcag atcagattca cgggtggaga
     7441 gggttatttt ctctttttta ttttttcttg cctgctcata tttgggcctg tgtgaacccc
     7501 aggctccgcc tcggccccca ggagccgctc ttatttaaag gggatcctcg ccttcagtct
     7561 tcgtcccagt ggaagcatca gtcttgtgat aacaacttgg tgagtcctac agaccagcgc
     7621 tcgactctag gctttaaatg cattgatttc tctggattag ctaccgggag agttttaact
     7681 ttgcttatta gtcttgtgct cgaactgcta ggtttaaaga gtgttttctc taatgcatgc
     7741 atgaagcgac agctgaaaac cagaaataga gcgtttgtcc tcctgttaaa ctctagcctt
     7801 tctttggcat ttgtcagtcc gagtcttcag ctgccggcag gagattgggt ttcagtaaca
     7861 cttgctgagc acaaactaca cgtgctttct tcagatttaa ttcattatta atcattctag
     7921 atttaaatga tgttcttact cattgctgat tattttagcc ttgcggagca gattaaatga
     7981 gcacgtgttt cattaagatt gatttaaata taattaatat ttcaccactg tagttttaaa
     8041 attgccttct aagtcatggt tgatttttaa taggtatcgg ctctaattag attactattt
     8101 tggtttttaa atgttatttc tatttcattt gtaggctcgt ttttaatgtg attttattag
     8161 attctagttt taaaatcatc tcttagcctt aatgagcaga ctgaatgaac atgtttcatt
     8221 aagattaact tcattataat gaattattta atcattctag tgggttataa atcagctcta
     8281 tttaaattaa ttgtcgtgtt tatttctatt taatttttga ttattttttc agggtcgttt
     8341 ttatgtgatt ttttcccgtt tctaatttta aactaattgt ttgattttta tatgattcga
     8401 ctctttttaa atgaattgtt ttgctttttt tattgagcat atatatatta tacaatttca
     8461 atcaacaatt gttgtgcttt ttaaatgttg tttctatttc atttctaggg tcgattttat
     8521 tcgattctag ttttaaaatc gtgattttta acttctaacc ttactgagca gactgaatga
     8581 acatgtttca tcaagattaa cttcattata gttaattatt gaatcgtgct agtggtttat
     8641 aaatcagctc tatttaaatt aatcatcatc attatttcta tttaattttt attatttctt
     8701 taggattatt tttatgtgat ttttttcacg ttctagtttt aaactcaatg ctcattactg
     8761 ctcacccttg ctgagcagtc tttcgttaag attaaattaa ttaataacca ttctagtttt
     8821 aaaacagtgt cttaaggcat agctgatatt ttacaagttt actatttaat atatctcctt
     8881 tcttttttta gggtcgattt catctgattt gattaatttt tattatcttc actaagcttt
     8941 ttgttctagg ttttattctc agtattatta ctgttatatt tgaactgaat ttctcctcat
     9001 agtttattca ttttatttat ttattttttg tttattcgct attttgtatt ttagttttta
     9061 attagaaatt ttattagaat cgttttgaac tagtttttat gcactctaac attaatatta
     9121 aatcataact attattttta ttataattta tttgtgttta tctttttgtg tagctttcat
     9181 gtgtttttat tttagtttta ttatgtttgc attgaatttt ttggactagt ttatataacg
     9241 atatacagaa aagccatgac aacaaatgtt tttataatac ctattaagta taagctaatc
     9301 agatttccac tattctgttc aaagttaaca ttattagcca ttctgatgga tcatttgaca
     9361 tttgtcatat gtctttatga acactgctct ttcagatgcc acttttaaag ctgtttttta
     9421 cagtaaatga acgtttatta ttatgacatt atttcctaag gcctctagag tacagcagga
     9481 tgtggcgtgt gcattaccgt gtaattatgc tgactgtaaa tgtgcattta tagactgtca
     9541 gcatgtctga ctgtgcgctg ttgtgattgg tcctgcacag accaag
//
