LOCUS       sequence-221276-        9599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab5aa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab5aa
FEATURES             Location/Qualifiers
     source          1..9599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1423..1443)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1451..1467)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1483..1617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1507..1526)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1561..1580
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2056..2243,2244..2255)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2289..2305)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2289..2305)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2342..2362)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2342..2360)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2381..2397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2394..2416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2405..2421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2429..2435,2436..2453,2454..2459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2474..2495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2612..2629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2783..3371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2863..2882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3542..4333,4334..4402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4165..4184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4403..4507)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4670..4691)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5040..5051,5052..5214)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5587..5603
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5623..5643
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggccaat aggggaggag caacacgccc
      781 caacgggtcc aacgcgggca ataagatctg ccagtttaaa ctggtcttgc taggagagtc
      841 agcggtgggg aagtccagcc tcgttctgcg cttcgtcaaa gggcagtttc acgagtttca
      901 ggaaagcaca ataggagcgg cctttcttac acagacactg tgcttggatg acacgacggt
      961 aaagtttgag atctgggaca cagctggaca ggagcgctac cacagtctgg cccccatgta
     1021 ctacagaggt gcccaggccg ccattgtagt ttatgacatc accaatgagg agtcatttgc
     1081 aagagcaaag aactgggtta aagagcttca gaggcaggct agcccaaaca ttgtcatcgc
     1141 tctgtctggg aacaaggctg accttgccaa caagagagct gtggacttcc aggatgctca
     1201 gtcttatgca gatgacaaca gtttgctgtt catggagacg tcagcaaaaa cctccatgaa
     1261 cgtgaacgag atcttcatgg ccattgcgaa aaagttgcct aagagtgagc cccaagctgc
     1321 cggagccaac agcgggcgga gtaggggtgt ggacctcaca gagacagccc aacccactaa
     1381 agccccctgc tgcagcaact aacgcaaaac aaaaatcccc gtcaacttta ttatacatag
     1441 ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga catgataaga
     1501 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1561 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1621 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1681 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctgc
     1741 gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag
     1801 ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt ataatttccc
     1861 taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca
     1921 aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag
     1981 aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca
     2041 agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt
     2101 gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat
     2161 tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta
     2221 atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa
     2281 gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
     2341 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2401 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2461 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2521 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2581 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3181 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3421 actcacgtta aggcattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3661 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3841 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3901 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3961 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4021 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4081 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4141 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4201 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4261 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4321 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4381 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4441 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4501 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     4561 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4621 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4681 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4741 gggcgatggc ccacgctggc taagaactca tcagcctccc cggtccatct acccacgtac
     4801 caatgcacca attggccaca atgacggcta ctacatggtg ccattccttc ctctttatag
     4861 gaatggagac tacctcctgt ccaacaatgc tcttggatac gagtacgcct acctgttgga
     4921 cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt gaatatctgt
     4981 tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg agttcttgac
     5041 agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa
     5101 ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa
     5161 gtactccatt aaaattgtac ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa
     5221 ctagagattc ttgtttaagc ttttaatctc aaaaaacatt aaatgaaatg catacaaggt
     5281 tttatcctgc tttagaactg tttgtattta attatcaaac tataagacag acaatctaat
     5341 gccagtacac gctactcaaa gttgtaaaac ctcagattta acttcagtag aagctgattc
     5401 tcaaaattgt tagtgtcaag cctagctctt ttggggctga aaagcaatcc tgcagtgctg
     5461 aaaagcctct cacaggcagc cgatgcggga agaggtgtat tagtcttgat agagaggctg
     5521 caaatagcag gaaacgtgag cagagactcc ctggtgtctg aaacacaggc cagatgggcc
     5581 ctcgagcagg aaacagctat gaccatgatt acgccaagct atcaactttg tatagaaaag
     5641 ttggcacgcg cagattccgt gtgggcctgg taatatctta ccattttaag ctattttaag
     5701 attagggaaa aaaattaaat aaaactactt taaaattact ttccttaaaa agcaacatat
     5761 ttatttagtt tttgtttaac tggctcaaga ttgtaatatt ttacttttac atgttttttt
     5821 aagtacctaa atgtgttcaa aagtaaagta tgttattgtt tttttttttt aatgttagct
     5881 aagctttaaa ttgtaatata ttacttttaa aagtgacttt ttaaaaatgt aaataaaaaa
     5941 gtaactataa atgtgtaatg catgttattt ctgtgggttt ttaattttca tctcaactgg
     6001 gcttacaatt ttaacataat aaaaatcaaa tcaaatcaaa tcaggccctt atttgtcaca
     6061 tacactcttg tatactgaaa taaactaaat gttgagaaaa atttaaatac accaaaataa
     6121 cgcatacctt ttttgtaatt acctaagtaa aacatttagt tcctttttta agtgactcaa
     6181 gattgtaatc tattaattgt aaaagataaa actagtgatc aatctgacgt gcttctatgc
     6241 agcctgtgag attaattgtg atgtttaatt attaaacaat ttaaatggat caaacatttc
     6301 acatgtgctt attgtgtcca acaaaaccag cagaagcact taaaaatatt gcatgacaac
     6361 atgagttttt aatgtaaatt atatatactt cctaaacgaa gttaatcata tttctaaaat
     6421 aataatgtca ttgaaatatt taaataaatg ataagcttta gttgattcaa cataaaattg
     6481 aaagcaataa ggactttttt tttttacagc ggtgaagcaa aagcatgtaa taatcatata
     6541 atataataaa taaataaata aataatggct cttggtgcaa atatgaacac tttaaataaa
     6601 tatagaataa atatgatcaa taagtcataa cgatgtgttt tatgttgatt ataatttaaa
     6661 catgtttcta acgcaagttt taaaaactgt tttaaagtat tattttaagt tataagatca
     6721 atttgactca acattttgta gtgaaaatat gttgtgtaaa attaatataa tatacactat
     6781 aaaaatgcag ggttccacac aatacattaa tgttgtccca acacaaatcg attaagttaa
     6841 caaatatatg tggattgaac ataaaaaatt aagttgtccc aatgaaatct caagaattgt
     6901 gttgtttcag ctcattttaa ataagtagtt ttaacaagta aaaaataaca tatttttgag
     6961 tgtgtatata tatatattac aaaatataat aatataaaat gtaaaagata taaaccatgc
     7021 tacatatatt taagagctat tttgcacatt acttttttgc aagccatttt agtgtttttt
     7081 ttacaaatat atattagcta aatatacaat atcttaaata tacaattaat cagacatgca
     7141 attaatgcat ttaacctaca taaaaataca tgatcaacat atgttttatg ctgattacaa
     7201 ctaaatcaaa cgtaatgaaa ttaatttata aaagcgattt aaatatgtca atattttaag
     7261 ttaaaatcac ttgaaaccta ctataaatga acttaaatta aaaaaatctc ctttaattcc
     7321 aatgcaacag tgttgttttt tcagctgagg acccccggtg cagatggttg gcccccggcc
     7381 cgctattcta aggaagcctt tcttaatttt agggcggcgc tgcatgcgct cagatcagat
     7441 tcacgggtgg agagggttat tttctctttt ttattttttc ttgcctgctc atatttgggc
     7501 ctgtgtgaac cccaggctcc gcctcggccc ccaggagccg ctcttattta aaggggatcc
     7561 tcgccttcag tcttcgtccc agtggaagca tcagtcttgt gataacaact tggtgagtcc
     7621 tacagaccag cgctcgactc taggctttaa atgcattgat ttctctggat tagctaccgg
     7681 gagagtttta actttgctta ttagtcttgt gctcgaactg ctaggtttaa agagtgtttt
     7741 ctctaatgca tgcatgaagc gacagctgaa aaccagaaat agagcgtttg tcctcctgtt
     7801 aaactctagc ctttctttgg catttgtcag tccgagtctt cagctgccgg caggagattg
     7861 ggtttcagta acacttgctg agcacaaact acacgtgctt tcttcagatt taattcatta
     7921 ttaatcattc tagatttaaa tgatgttctt actcattgct gattatttta gccttgcgga
     7981 gcagattaaa tgagcacgtg tttcattaag attgatttaa atataattaa tatttcacca
     8041 ctgtagtttt aaaattgcct tctaagtcat ggttgatttt taataggtat cggctctaat
     8101 tagattacta ttttggtttt taaatgttat ttctatttca tttgtaggct cgtttttaat
     8161 gtgattttat tagattctag ttttaaaatc atctcttagc cttaatgagc agactgaatg
     8221 aacatgtttc attaagatta acttcattat aatgaattat ttaatcattc tagtgggtta
     8281 taaatcagct ctatttaaat taattgtcgt gtttatttct atttaatttt tgattatttt
     8341 ttcagggtcg tttttatgtg attttttccc gtttctaatt ttaaactaat tgtttgattt
     8401 ttatatgatt cgactctttt taaatgaatt gttttgcttt ttttattgag catatatata
     8461 ttatacaatt tcaatcaaca attgttgtgc tttttaaatg ttgtttctat ttcatttcta
     8521 gggtcgattt tattcgattc tagttttaaa atcgtgattt ttaacttcta accttactga
     8581 gcagactgaa tgaacatgtt tcatcaagat taacttcatt atagttaatt attgaatcgt
     8641 gctagtggtt tataaatcag ctctatttaa attaatcatc atcattattt ctatttaatt
     8701 tttattattt ctttaggatt atttttatgt gatttttttc acgttctagt tttaaactca
     8761 atgctcatta ctgctcaccc ttgctgagca gtctttcgtt aagattaaat taattaataa
     8821 ccattctagt tttaaaacag tgtcttaagg catagctgat attttacaag tttactattt
     8881 aatatatctc ctttcttttt ttagggtcga tttcatctga tttgattaat ttttattatc
     8941 ttcactaagc tttttgttct aggttttatt ctcagtatta ttactgttat atttgaactg
     9001 aatttctcct catagtttat tcattttatt tatttatttt ttgtttattc gctattttgt
     9061 attttagttt ttaattagaa attttattag aatcgttttg aactagtttt tatgcactct
     9121 aacattaata ttaaatcata actattattt ttattataat ttatttgtgt ttatcttttt
     9181 gtgtagcttt catgtgtttt tattttagtt ttattatgtt tgcattgaat tttttggact
     9241 agtttatata acgatataca gaaaagccat gacaacaaat gtttttataa tacctattaa
     9301 gtataagcta atcagatttc cactattctg ttcaaagtta acattattag ccattctgat
     9361 ggatcatttg acatttgtca tatgtcttta tgaacactgc tctttcagat gccactttta
     9421 aagctgtttt ttacagtaaa tgaacgttta ttattatgac attatttcct aaggcctcta
     9481 gagtacagca ggatgtggcg tgtgcattac cgtgtaatta tgctgactgt aaatgtgcat
     9541 ttatagactg tcagcatgtc tgactgtgcg ctgttgtgat tggtcctgca cagaccaag
//
