LOCUS       sequence-22128-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Wang L, Harris TE, Roth RA, Lawrence JC
  TITLE     PRAS40 regulates mTORC1 kinase activity by functioning as a direct 
            inhibitor of substrate binding.
  JOURNAL   J Biol Chem. 2007 Jul 6. 282(27):20036-44.
  PUBMED    17510057
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 6. 282(27):20036-44."
COMMENT     Sequence Label: PRAS40 (mouse) shRNA
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    143..260
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        309..819
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     738..756
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(843..862)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tattcgattc ttggctttta tatcttgtgg aaaggacgaa acaccggtga agctgaagcg
       61 gaaatattac tcgagtaata tttccgcttc agcttctttt tcaattctcg acctcgagac
      121 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
      181 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
      241 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
      301 ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
      361 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
      421 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
      481 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
      541 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
      601 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
      661 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
      721 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
      781 ggctccctcg ttgaccgaat caccgacctc tctccccagg ggggatccac cgggagctta
      841 ccatgaccga gtacaagccc acggtgcgct cgcaccgcga cactcccagg gcgtaag
//
