LOCUS       sequence-221284-        9560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab6a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab6a
FEATURES             Location/Qualifiers
     source          1..9560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1384..1404)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1412..1428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1444..1578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1468..1487)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1522..1541
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2017..2204,2205..2216)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2250..2266)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2250..2266)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2303..2323)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2303..2321)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2342..2358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2355..2377)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2366..2382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2390..2396,2397..2414,2415..2420))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2435..2456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2573..2590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2744..3332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2824..2843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3503..4294,4295..4363))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4126..4145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4364..4468)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4631..4652)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5001..5012,5013..5175)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5548..5564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5584..5604
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgtctgca gcaggagatt tcggcaaccc
      781 gctccgcaaa ttcaagctag tttttctggg agagcagagc gtgggaaaga cgtcgttgat
      841 taccagattt atgtacgaca gtttcgataa cacctaccag gccacgatag gaattgattt
      901 cttgtcgaaa accatgtgtc ttgaggacag aacaatcagg ctgcagcttt gggacacagc
      961 aggacaggag cgtttccgta gcctcattcc cagttacatc cgagattcag ccgctgctgt
     1021 tgtagtttac gacatcacaa atgtcaactc attccagcag accaccaaat ggatcgatga
     1081 cgtccgaacg gaaagaggaa gtgatgtcat catcatgcta gtgggaaaca aaacagatct
     1141 tgcagacaaa aggcaagtgt ctatcgagga aggagaaagg aaagccaaag aactgaatgt
     1201 aatgtttatt gaaactagtg ctaaagccgg atacaacgtc aagcagcttt tccgccgtgt
     1261 agcagcagcc cttcctggta tggagagcac acaagacaag agcagagagg acatgatcga
     1321 cataaaattg gaaaagccac cagagcagcc ggtcagcgaa ggcggctgtt catgctgagg
     1381 cttcaacttt attatacata gttgataatt cactggccgt cgttttacgg taccatcgat
     1441 gatgatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     1501 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     1561 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     1621 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     1681 gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc tgtggctgat
     1741 tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact
     1801 gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga
     1861 tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt
     1921 gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat
     1981 tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac
     2041 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
     2101 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
     2161 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc
     2221 ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc
     2281 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     2341 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     2401 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     2461 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     2521 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2581 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2641 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2701 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2761 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2821 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2881 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2941 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3001 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3061 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3121 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3181 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3241 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3301 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3361 ctgacgctca gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga ttatcaaaaa
     3421 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3481 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3541 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3601 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3661 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3721 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3781 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3841 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3901 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3961 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4021 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4081 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4141 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4201 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4261 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4321 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4381 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4441 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     4501 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     4561 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     4621 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     4681 agggcgaaaa accgtctatc agggcgatgg cccacgctgg ctaagaactc atcagcctcc
     4741 ccggtccatc tacccacgta ccaatgcacc aattggccac aatgacggct actacatggt
     4801 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaatg ctcttggata
     4861 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
     4921 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
     4981 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
     5041 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
     5101 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
     5161 aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttttaatct caaaaaacat
     5221 taaatgaaat gcatacaagg ttttatcctg ctttagaact gtttgtattt aattatcaaa
     5281 ctataagaca gacaatctaa tgccagtaca cgctactcaa agttgtaaaa cctcagattt
     5341 aacttcagta gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct tttggggctg
     5401 aaaagcaatc ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta
     5461 ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc cctggtgtct
     5521 gaaacacagg ccagatgggc cctcgagcag gaaacagcta tgaccatgat tacgccaagc
     5581 tatcaacttt gtatagaaaa gttggcacgc gcagattccg tgtgggcctg gtaatatctt
     5641 accattttaa gctattttaa gattagggaa aaaaattaaa taaaactact ttaaaattac
     5701 tttccttaaa aagcaacata tttatttagt ttttgtttaa ctggctcaag attgtaatat
     5761 tttactttta catgtttttt taagtaccta aatgtgttca aaagtaaagt atgttattgt
     5821 tttttttttt taatgttagc taagctttaa attgtaatat attactttta aaagtgactt
     5881 tttaaaaatg taaataaaaa agtaactata aatgtgtaat gcatgttatt tctgtgggtt
     5941 tttaattttc atctcaactg ggcttacaat tttaacataa taaaaatcaa atcaaatcaa
     6001 atcaggccct tatttgtcac atacactctt gtatactgaa ataaactaaa tgttgagaaa
     6061 aatttaaata caccaaaata acgcatacct tttttgtaat tacctaagta aaacatttag
     6121 ttcctttttt aagtgactca agattgtaat ctattaattg taaaagataa aactagtgat
     6181 caatctgacg tgcttctatg cagcctgtga gattaattgt gatgtttaat tattaaacaa
     6241 tttaaatgga tcaaacattt cacatgtgct tattgtgtcc aacaaaacca gcagaagcac
     6301 ttaaaaatat tgcatgacaa catgagtttt taatgtaaat tatatatact tcctaaacga
     6361 agttaatcat atttctaaaa taataatgtc attgaaatat ttaaataaat gataagcttt
     6421 agttgattca acataaaatt gaaagcaata aggacttttt ttttttacag cggtgaagca
     6481 aaagcatgta ataatcatat aatataataa ataaataaat aaataatggc tcttggtgca
     6541 aatatgaaca ctttaaataa atatagaata aatatgatca ataagtcata acgatgtgtt
     6601 ttatgttgat tataatttaa acatgtttct aacgcaagtt ttaaaaactg ttttaaagta
     6661 ttattttaag ttataagatc aatttgactc aacattttgt agtgaaaata tgttgtgtaa
     6721 aattaatata atatacacta taaaaatgca gggttccaca caatacatta atgttgtccc
     6781 aacacaaatc gattaagtta acaaatatat gtggattgaa cataaaaaat taagttgtcc
     6841 caatgaaatc tcaagaattg tgttgtttca gctcatttta aataagtagt tttaacaagt
     6901 aaaaaataac atatttttga gtgtgtatat atatatatta caaaatataa taatataaaa
     6961 tgtaaaagat ataaaccatg ctacatatat ttaagagcta ttttgcacat tacttttttg
     7021 caagccattt tagtgttttt tttacaaata tatattagct aaatatacaa tatcttaaat
     7081 atacaattaa tcagacatgc aattaatgca tttaacctac ataaaaatac atgatcaaca
     7141 tatgttttat gctgattaca actaaatcaa acgtaatgaa attaatttat aaaagcgatt
     7201 taaatatgtc aatattttaa gttaaaatca cttgaaacct actataaatg aacttaaatt
     7261 aaaaaaatct cctttaattc caatgcaaca gtgttgtttt ttcagctgag gacccccggt
     7321 gcagatggtt ggcccccggc ccgctattct aaggaagcct ttcttaattt tagggcggcg
     7381 ctgcatgcgc tcagatcaga ttcacgggtg gagagggtta ttttctcttt tttatttttt
     7441 cttgcctgct catatttggg cctgtgtgaa ccccaggctc cgcctcggcc cccaggagcc
     7501 gctcttattt aaaggggatc ctcgccttca gtcttcgtcc cagtggaagc atcagtcttg
     7561 tgataacaac ttggtgagtc ctacagacca gcgctcgact ctaggcttta aatgcattga
     7621 tttctctgga ttagctaccg ggagagtttt aactttgctt attagtcttg tgctcgaact
     7681 gctaggttta aagagtgttt tctctaatgc atgcatgaag cgacagctga aaaccagaaa
     7741 tagagcgttt gtcctcctgt taaactctag cctttctttg gcatttgtca gtccgagtct
     7801 tcagctgccg gcaggagatt gggtttcagt aacacttgct gagcacaaac tacacgtgct
     7861 ttcttcagat ttaattcatt attaatcatt ctagatttaa atgatgttct tactcattgc
     7921 tgattatttt agccttgcgg agcagattaa atgagcacgt gtttcattaa gattgattta
     7981 aatataatta atatttcacc actgtagttt taaaattgcc ttctaagtca tggttgattt
     8041 ttaataggta tcggctctaa ttagattact attttggttt ttaaatgtta tttctatttc
     8101 atttgtaggc tcgtttttaa tgtgatttta ttagattcta gttttaaaat catctcttag
     8161 ccttaatgag cagactgaat gaacatgttt cattaagatt aacttcatta taatgaatta
     8221 tttaatcatt ctagtgggtt ataaatcagc tctatttaaa ttaattgtcg tgtttatttc
     8281 tatttaattt ttgattattt tttcagggtc gtttttatgt gattttttcc cgtttctaat
     8341 tttaaactaa ttgtttgatt tttatatgat tcgactcttt ttaaatgaat tgttttgctt
     8401 tttttattga gcatatatat attatacaat ttcaatcaac aattgttgtg ctttttaaat
     8461 gttgtttcta tttcatttct agggtcgatt ttattcgatt ctagttttaa aatcgtgatt
     8521 tttaacttct aaccttactg agcagactga atgaacatgt ttcatcaaga ttaacttcat
     8581 tatagttaat tattgaatcg tgctagtggt ttataaatca gctctattta aattaatcat
     8641 catcattatt tctatttaat ttttattatt tctttaggat tatttttatg tgattttttt
     8701 cacgttctag ttttaaactc aatgctcatt actgctcacc cttgctgagc agtctttcgt
     8761 taagattaaa ttaattaata accattctag ttttaaaaca gtgtcttaag gcatagctga
     8821 tattttacaa gtttactatt taatatatct cctttctttt tttagggtcg atttcatctg
     8881 atttgattaa tttttattat cttcactaag ctttttgttc taggttttat tctcagtatt
     8941 attactgtta tatttgaact gaatttctcc tcatagttta ttcattttat ttatttattt
     9001 tttgtttatt cgctattttg tattttagtt tttaattaga aattttatta gaatcgtttt
     9061 gaactagttt ttatgcactc taacattaat attaaatcat aactattatt tttattataa
     9121 tttatttgtg tttatctttt tgtgtagctt tcatgtgttt ttattttagt tttattatgt
     9181 ttgcattgaa ttttttggac tagtttatat aacgatatac agaaaagcca tgacaacaaa
     9241 tgtttttata atacctatta agtataagct aatcagattt ccactattct gttcaaagtt
     9301 aacattatta gccattctga tggatcattt gacatttgtc atatgtcttt atgaacactg
     9361 ctctttcaga tgccactttt aaagctgttt tttacagtaa atgaacgttt attattatga
     9421 cattatttcc taaggcctct agagtacagc aggatgtggc gtgtgcatta ccgtgtaatt
     9481 atgctgactg taaatgtgca tttatagact gtcagcatgt ctgactgtgc gctgttgtga
     9541 ttggtcctgc acagaccaag
//
