LOCUS       sequence-221310-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-X2 where both of the consensus motif's lysines are mutated to 
            arginine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X2DM
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     113..129
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     113..129
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(179..207)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttattaatct tttgtttcct cgtcattgtt ctcgttccct ttcttccttg tttctttttc
       61 tgcacaatat ttcaagctat accaagcata caatcaactc caagctggcc gctctagaac
      121 tagtggatcc cccgggctgc aggaattcat gagtaaagga gaagaacttt tcactggagt
      181 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      241 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcantnctgg
      301 aaaactacct gttccatggc caacacttgt cactactttc acttatggtg ttcaatgctt
      361 ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg
      421 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
      481 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
      541 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
      601 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
      661 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
      721 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
//
