LOCUS       sequence-221312-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-X2 where both of the consensus motif's lysines are mutated to 
            arginine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X2DM
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     99..117
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     135..151
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     136..152
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(240..260)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     855..883
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgttcttata ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta
       61 actccttcct tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata
      121 actaattaca tgactcgagg tcgacggtat cgataagctt ttaaggcctc ctagatgatc
      181 caggtcttct tgaagaacct ttgtatagtt catccatgcc atgtgtaatc ccagcagctg
      241 ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagggcag
      301 attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat
      361 tttgttgata atggtctgct agttgaacgc ttccatcttc aatgttgtgt ctaattttga
      421 agttaacttt gattccattc ttttgtttgt ctgccatgat gtatacattg tgtgagttat
      481 agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca atacctttta
      541 actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacgt gtcttgtagt
      601 tcccgtcatc tttgaaaaat atagttcttt cctgtacata accttcgggc atggcactct
      661 tgaaaaagtc atgccgtttc atatgatctg ggtatcttga aaagcattga acaccataag
      721 tgaaagtagt gacaagtgtt ggccatggaa caggtagttt tccagtagtg caaataaatt
      781 tnnnnnnaag ttttccgtat gttgcatcac cttcaccctc tccactgaca gaaaatttgt
      841 gcccattaac atcaccatct aattcaacaa gaattgggac aact
//
