LOCUS       sequence-221319-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Spt2-GFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: Spt2-GFP
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..38
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     23..39
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(61..226)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     147..171
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(290..310)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagctggtac cgggcccccc ctcgaggtcg acggtatcga tgtagaggtg tggtcaataa
       61 gagcgacctc atactatacc tgagaaagca acctgaccta caggaaagag ttactcaaga
      121 ataagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt
      181 ttataactta tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgg
      241 cgcgccctat ttgtatagtt catccatgcc atgtgtaatc ccagcagctg ttacaaactc
      301 aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagggcag attgtgtgga
      361 caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata
      421 atggtctgct agttgaacgc ttccatcttc aatgttgtgt ctaattttga agttaacttt
      481 gattccattc ttttgtttgt ctgccatgat gtatacattg tgtgagttat agttgtattc
      541 caatttgtgt ccaagaatgt ttccatcttc tttaaaatca atacctttta actcgattct
      601 attaacaagg gtatcacctt caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc
      661 tttgaaaaat atagttcttt cctgtacata accttcgggc atggcactct tgaaaaagtc
      721 atgccgtttc atatgatctg ggtatcttga aaagcattga acacc
//
