LOCUS       sequence-221320-        4757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: LOC5567066 Gastrin-releasing peptide receptor
FEATURES             Location/Qualifiers
     source          1..4757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2645..2779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2682..2701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2736..2755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2818..2835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2989..3577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3069..3088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3748..4539,4540..4608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4371..4390
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4609..4713)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4753..4757,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgccacagat cccggagtat atacgggcga catcgatggt
      841 gttctgcatc atcatcatgt gcctgggtgt catcggaaat attatggtcc cgatcgtcat
      901 cctcaagacg aaggatatgc gcaactcgac caacatcttc ctaaccaacc taagcatcgc
      961 tgacctcctt gttttgctcg tctgcacacc aaccgtactt gttgaggtga actccccacc
     1021 ggaggtgtgg gtactgggag aagaaatgtg taaagcggta cccttcgtgg agctgacggt
     1081 ggcccatgcg agtgtcctga cgatcctggc gatatccttc gagcggtact atgccatctg
     1141 tgaaccgctc aaggccggtt acgtctgcac caaggctcgg gctctgatga tttgtctggc
     1201 cgcgtggacc gtggcagcca tcctgacgag cccgattctg atgatctcat cgtatgaata
     1261 cgtggagtac gtcaacggaa aaatggtcgc ctcgtgtctc acgccagtgg aaaacttttg
     1321 gcacgccacc ttctttgtgg ccagcatcgt ggtgttcttt atcgtaccac tcctgattct
     1381 ggtggttctg tattccgtga ttgcgaaaaa tctgatggac aatcccggta tcataatgtc
     1441 caatgccagc ggcaatcgga acaatgtcat caaatatcgc aaacaggtca tcttcatgct
     1501 cggagcagtc gtggtcagct ttttcttctg cctacttccg ttccgggcac ttaccttgtg
     1561 gatcataatt gtgcccagcg agaacatcat ttcgattggg attgaacggt tctacattct
     1621 gctgtacttt tgccgaataa tgctgtatat gaactcggcc atcaatccca tactgtacaa
     1681 tttgatgtcg tccaagttcc ggaatgggtt cctgcagctg ttgggatgtg gcaaaataat
     1741 ccgatcggat agcatttctt ctggtgccag gaagggaact ttccacacgg catcgacaaa
     1801 tctcagcagt tctcacaata gttcctacca gagacggttt accagagaag gaagttgcat
     1861 aagcggaagc attcgagcgg tttcagacaa tagccggaga cgattacttc accagcggca
     1921 cagtagtacg atatcgatca gatcatccca tgacgaacat agcggaacag gaggtggtgg
     1981 aagtggagtc ccaattcaaa ccggaaacgg cacaatcgaa accactccac tgaaggcgga
     2041 caaaattgaa gaactagacg aaacgacaaa ctcactgcca aatggttacg gtttcaatag
     2101 gtttaagcat gtcgccagcg cagccggtgg caatagttgt aatgttagct ttaagactac
     2161 aaatagctgt aacggcaaga ctagtcatcg gatgaatgga acagtttcgg tagaaccgaa
     2221 agccgttcac caagcggaca ataaatatct tatagtaagg gcagcagtag tagtgatgcg
     2281 ggaggtatcc gaggaaaacg gaaagccgct tccgctgtcg tcttctccaa agtcgccgcc
     2341 agcagaaaac ggttcgacgg aagcggaagt tacgctaatt cagagtgcac gcggaagtga
     2401 gcaaatagtg tctatcatca ctgccaaaga gcgagcgaat ggaaactgtg tcaataggac
     2461 acagagagct gatacgaaga atacgaacga ggatgcggta gcttttgatg agattaataa
     2521 aatacggcag ttctacggct cgatggaatg tgatatctag gcggccgcta gactagtcta
     2581 gagaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     2641 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2701 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2761 atcttatcat gtctggatcc ccgggtaccg agctcgaatt aattcctctt ccgcttcctc
     2821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2941 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3001 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3061 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3121 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3181 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3241 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3301 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3361 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3421 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3481 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3541 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3601 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3661 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3721 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3781 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3841 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3901 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3961 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4021 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4081 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4141 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4201 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4261 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4321 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4381 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4441 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4501 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4561 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4621 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4681 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc
     4741 aagcttggct gtggaat
//
