LOCUS       sequence-221321-        4328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: LOC110681163 Neuropeptide FF receptor
FEATURES             Location/Qualifiers
     source          1..4328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2216..2350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2253..2272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2307..2326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2389..2406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2560..3148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2640..2659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3319..4110,4111..4179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3942..3961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4180..4284)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4324..4328,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tgaaaaccgg gttcagttta caaccggagg acgcatctct
      841 tttgagagtt cctgccccac ggtttttttg ggccatgcca acagcgatca acacatcgca
      901 acattcttac cctatggaag tgtgggatca aattccagac aaagacatca ttttaaagct
      961 gtccgttttg ataccgattg tactgtttgg catattgggt aacgtttccc tcttggaaat
     1021 tattttctcc aatcgatcat tgcgcacacc aacgcatatg ttaatagcaa atctcgccct
     1081 cattgatttg gtgacgttgt tgatttgtcc accaatgttc atattgcacg acattcatca
     1141 gagttacgta ctaggaccaa ttggatgtaa gctggaagga tttgttgaag gtggacttct
     1201 tatcacttct attttggctc tctgtgtcgt aagttacgat cgtttggcag caattgttct
     1261 atccagaaaa gcgagattca aaatgagtag cgttattgta gcatcgattg ggtgctggat
     1321 tttagggttt gttgctgctt tgcccttagc aatttatcga aacttcaaag aaagagactg
     1381 gagcaatttt cacgaaacat tttgctcgga agataaacat gtgatgccac tatactggga
     1441 ctatataatt atcttgttgg tttggcttcc gttgagtgtc atgatggtta cttacagtac
     1501 gatcttgtgt aaactagatc ggtatgaaag aaaagcaatg aacagggagc atccaatggt
     1561 tgtaaggtac aaaagtcgtg tggccaaaac attatttatt gtacttatta ccttcataat
     1621 tgtaaggatt cctttcaccg ttatgatcct gatctactac aaggatgtcg acgataataa
     1681 tagttttgcg ataagtgaaa gcttcattat gctttggtgg tttgccaaag ttgctttcat
     1741 attcttgtat tctgcaatga atccgatcat ttatggattg accaatagaa catttcggag
     1801 agcattcagg gattcgagaa tccttgggac acttttacgc ttcagcgacg atgagcaagt
     1861 tgatgaaaaa ccaaaaaagc caaggatcat atcattttcg aagaaaacgc atactgaagg
     1921 aatgatttca aaaagaggaa tcaaaagacg atcggctact aactggctaa attccacaat
     1981 aagatggaag gataggcgtt acaataatag aaaagtggaa aacaataacc cggaagctaa
     2041 tggaacacaa tcatcggaaa caggagcgag gaatgcgtct caggaacaga aagcctcagt
     2101 caatgagcca ataatcgcta agctgacgta agcggccgct agactagtct agagaaaaaa
     2161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     2221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2341 tgtctggatc cccgggtacc gagctcgaat taattcctct tccgcttcct cgctcactga
     2401 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2461 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2521 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2581 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2641 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2701 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2761 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2821 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2881 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3001 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3061 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg caagcttggc
     4321 tgtggaat
//
