LOCUS       sequence-221325-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses yeast Spt2-GFP fusion protein mutated from lysine to 
            arginine at site 166.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: Spt2-K166R-GFP
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(387..415)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aatcaagata ccaggatgac tatgatgaag aagataacga tatggatgat tttatagaag
       61 acgatgaaga tgaaggttac cacagcaaat cgaaacacag caatggtccc ggatatgatc
      121 gtgacgaaat ttgggctatg ttcaatagag gcaagaagcg gtcagaatac gattacgatg
      181 agcttgagga tgatgatatg gaagcaaatg agatggaaat cttggaagag gaggaaatgg
      241 caagaaaaat ggcaaggtta gaggataaac gtgaggaagc ttggttaaaa aagcatgaag
      301 aggagaagag acgccgtaag aagggcatac gcggtcgacg gatccccggg ttaattaaca
      361 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      421 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      481 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      541 ctactttcac ttatggtgtt caatgctttt caagataccc agatcatatg aaacggcatg
      601 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
      661 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
      721 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
      781 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag nntggaannc
      841 aagttaactt caaaattaga cacaacattg a
//
